electrode implantation in mouse hippocampus and prefrontal cortex for recording local field potentials

小鼠大脑中的 LFP 记录用于认知研究和药物分析

Local field potentials (LFPs) refer to the electrical activity recorded from the extracellular space, reflecting the collective activity of a localized group of neurons. They exhibit a diverse range of frequencies, spanning from slow waves at 1 Hz to fast oscillations at 100 Hz or 200 Hz. Specific frequency bands have been associated with cognitive functions such as learning, memory, and decision making1,2. Changes in LFP properties have been used as biomarkers for various neurological disorders, including dementia and schizophrenia3,4. Analyzing LFP recordings can offer valuable insights into the underlying pathological mechanisms associated with these conditions and potential therapeutic strategies.
Dual LFP recording is a technique used to measure localized electrical activity within and between two specific brain regions. This technique provides a valuable opportunity to investigate the intricate neural dynamics and signal communication occurring within and between distinct brain regions. Prior studies have revealed that detecting alterations in the neuronal properties of individual brain regions can be complex, but changes in interregional cortical communication can be observed5,6. Therefore, the utilization of dual LFP recording offers a potent means to address this issue.
Hippocampal-prefrontal connectivity plays a crucial role in modulating cognitive functions, and dysfunction has been linked to various neurological disorders7,8. Dual electrode recordings of these regions can provide information about these interactions. Unfortunately, there is limited information available on methods for performing dual electrode LFP recordings between these areas. Moreover, commercially available recording devices are generally expensive and lack adaptability to specific experimental designs. The conventional method for recording LFPs involves using a shielded cable to connect the recording device to electrodes implanted in an animal's brain. However, this approach is susceptible to motion artifacts and environmental noise, impacting the quality and reliability of the recorded signals.
This protocol describes a comprehensive procedure for performing dual-electrode LFP recordings in the mouse hippocampus and prefrontal cortex, using a low-cost custom-designed headstage that can be placed on the animal's head. These methods enable researchers to investigate region-specific oscillatory patterns within two discrete cerebral regions and explore interregional information exchange and connectivity between these areas.

作者聚焦：使用双 LFP 记录方案研究药物对小鼠大脑信号的影响

小鼠海马体和前额叶皮层中的双重细胞外记录

This study presents a comprehensive protocol for performing dual-electrode LFP recordings in the mouse hippocampus and the prefrontal cortex to investigate the effects of drugs on LFP properties in these areas. This protocol provides a low-cost and ultra-light brain signal recording device that is fully optimized for cognitive research in mice. This study provides a means to record signals with high signal-noise ratios in different brain regions, facilitating the investigation of interregional information communication within the brain.

电极植入小鼠海马体和前额叶皮层以记录局部场电位

首先，将麻醉的小鼠放在加热垫上，并将其固定在立体定位框架中。沿着头皮的中线做一个 8 毫米的切口，然后从该区域去除结缔组织。将 bregma 和 lambda 标志对齐在同一水平，以实现准确的电极放置。然后为参比电极或接地电极、锚螺钉和有源电极钻孔作为特定坐标。将定制的电极连接到立体定位框架臂上，并确保其垂直于大脑。将电极植入海马体 CA-1 区域。然后使用强力粘合剂和牙科粘结剂固定电极。植入两个 1.2 毫米的地脚螺钉以防止移动。接下来，将参比电极或接地电极直接与硬脑膜接触，位于 Lambda 地标后方 2 毫米和单侧 2 毫米处。将电极的黄铜管侧连接到多通道插座连接器。将接地电极放在中间。在中间引脚的外部使用0.8毫米的热缩管进行隔离。用粘合剂和牙科粘结剂固定电极、锚固件螺钉和连接器，让动物恢复。

electrode-implantation-mouse-hippocampus-prefrontal-cortex-for

Research

JoVE Journal

Neuroscience

Electrode Implantation in Mouse Hippocampus and Prefrontal Cortex for Recording Local Field Potentials

194 次观看.  The University of Melbourne. 首先，将麻醉的小鼠放在加热垫上，并将其固定在立体定位框架中。沿着头皮的中线做一个 8 毫米的切口，然后从该区域去除结缔组织。将 bregma 和 lambda 标志对齐在同一水平，以实现准确的电极放置。然后为参比电极或接地电极、锚螺钉和有源电极钻孔作为特定坐标。将定制的电极连接到立体定位框架臂上，并确保其垂直于大脑。将电极植入海马体 CA-1 区域。然?...

视频: 电极植入小鼠海马体和前额叶皮层以记录局部场电位

Dual Extracellular Recordings in the Mouse Hippocampus and Prefrontal Cortex

The technique of recording local field potentials (LFPs) is an electrophysiological method used to measure the electrical activity of localized neuronal populations. It serves as a crucial tool in cognitive research, particularly in brain regions like the hippocampus and prefrontal cortex. Dual LFP recordings between these areas are of particular interest as they allow the exploration of interregional signal communication. However, methods for performing these recordings are rarely described, and most commercial recording devices are either expensive or lack adaptability to accommodate specific experimental designs. This study presents a comprehensive protocol for performing dual-electrode LFP recordings in the mouse hippocampus and the prefrontal cortex to investigate the effects of antipsychotic drugs and potassium channel modulators on LFP properties in these areas. The technique enables the measurement of LFP properties, including power spectra within each brain region and coherence between the two. Additionally, a low-cost, custom-designed recording device has been developed for these experiments. In summary, this protocol provides a means to record signals with high signal-to-noise ratios in different brain regions, facilitating the investigation of interregional information communication within the brain.

This protocol outlines the use of a custom-designed recording device and electrodes to record local field potentials and investigate information flow in the mouse hippocampus and prefrontal cortex.

The technique of recording local field potentials (LFPs) is an electrophysiological method used to measure the electrical activity of localized neuronal ...