microbiological rapid on-site evaluation using dq-staining for clinical decisions

使用 M-ROSE 加强肺部感染治疗

The technique of rapid on-site evaluation (ROSE) is a highly efficient method employed in the field of pulmonary disease procedures. It enables real-time sampling and diagnostic intervention, facilitating immediate cytological analysis1. This innovative approach involves imprinting a portion of the tissue specimen onto a slide while preserving its integrity. One of the key advantages of ROSE lies in its capacity to facilitate prompt interpretation of clinical information through specialized microscopy techniques. This encompasses the analysis of cell morphology, classification, quantification, determination of constituent ratios, assessment of arrangement, correlation analysis, evaluation of background, and identification of foreign objects. By integrating all this data with the patient's clinical information, ROSE plays a pivotal role in evaluating sampling adequacy and guiding real-time interventional procedures and techniques2.
As a subsidiary of ROSE technology, M-ROSE technology primarily focuses on acquiring the microbiological background of target lesions rather than discerning between benign and malignant cells3,4. On one hand, M-ROSE enables the microscopic identification of pathogens such as Aspergillus, Cryptococcus, Pneumocystis, and Candida5. On the other hand, it holds significant guiding implications in assessing respiratory specimen quality, distinguishing infectious from non-infectious diseases, discriminating infection from contamination, as well as evaluating infection severity and prognosis6,7. For instance, within a respiratory specimen, the coexistence of bacteria exhibiting identical morphology alongside infiltrating inflammatory cells indicates an infection; conversely, the presence of multiple morphologically diverse bacteria accompanied by epithelial cells suggests contamination. The capacity to comprehensively analyze clinical information and predict outcomes renders M-ROSE an invaluable tool in pulmonary disease procedures.
In conclusion, ROSE serves as a crucial cytological carrier that significantly enhances the efficiency and precision of diagnosing lung diseases. Its multifaceted capabilities contribute to improved patient outcomes by ensuring timely intervention and facilitating accurate diagnosis through real-time sampling and diagnostic intervention techniques. However, it is important to note that these advancements rely on obtaining qualified samples. Herein, we present a standardized M-ROSE protocol encompassing slide preparation, staining techniques, and interpretation guidelines. This protocol serves as an invaluable reference for clinicians to establish accurate evaluation and treatment plans while facilitating decision-making regarding subsequent handling of target specimens.

作者聚焦：使用 M-ROSE 实时推进病原体检测和疾病评估

肺部感染性疾病的微生物学快速现场评估

Our intent is to introduce a real time rapid assessment method, M-ROSE to assist the physicians for formulating accurate treatment strategies for patient with pulmonary infections. Rapid cell staining technology and a high definition microscopic imaging system are currently used to advance research in the field. The number of clinical studies on M-ROSE is relatively limited.The biggest challenge is interpretation of M-ROSE results, which requires personnel, and trained personnel as it is difficult to visually identify pathogens and differentiate between infection and contamination. With a better understanding of pathogens causing infections in real time, physicians can avoid unnecessary broad spectrum antibiotics contributing to antibiotic resistance and as a treatment of pulmonary infections. M-ROSE is real time and highly efficient.M-ROSE enables the microscopic identification of pathogens such as aspergillus, cryptococcus, numerous and candida. It holds significant guiding implications in sizing respiratory basic quality, distinguishing infections from non-infectious disease, discriminating, infection from contamination, as well as evaluating infection, severity, and prognose.

使用 DQ 染色进行微生物学快速现场评估，用于临床决策

首先，获取 M-ROSE 所需的所有材料，并将全套 Diff-Quik 或 DQ 染色溶液放入带密封盖的玻璃染色罐中。对于卷状载玻片制备，使用 2.5 至 5 毫升一次性注射器针头提取组织颗粒。然后从细胞学载玻片的内侧到外侧 1/3 部分铺展一个中等厚度的圆形区域。将玻片浸入 Diff-A 溶液中 10 至 30 秒。要去除多余的 Diff-A 溶液，请将载玻片放入 PBS 染浴中，并轻轻上下摇晃。然后轻轻甩掉任何残留的缓冲液。将玻片浸入 Diff-B 溶液中 20 至 40 秒。然后将载玻片放入水染槽中，稍微上下摇晃。最后，使用吸水纸擦去载玻片上的残留液体，然后进行显微镜检查。使用 DQ 染色对患者支气管镜活检样本进行 M-ROSE 鉴定出肺孢子菌，其特征是结构清晰、对比鲜明和 ASCUS。

microbiological-rapid-on-site-evaluation-using-dq-staining-for

Research

JoVE Journal

Medicine

Microbiological Rapid On-Site Evaluation Using DQ-Staining for Clinical Decisions

41 次观看.  Chongqing Hospital of Traditional Chinese Medicine. 首先，获取 M-ROSE 所需的所有材料，并将全套 Diff-Quik 或 DQ 染色溶液放入带密封盖的玻璃染色罐中。对于卷状载玻片制备，使用 2.5 至 5 毫升一次性注射器针头提取组织颗粒。然后从细胞学载玻片的内侧到外侧 1/3 部分铺展一个中等厚度的圆形区域。将玻片浸入 Diff-A 溶液中 10 至 30 秒。要去除多余的 Diff-A 溶液，请将载玻片放入 PBS 染浴中，并轻轻上下摇晃。然后轻轻甩?...

视频: 使用 DQ 染色进行微生物学快速现场评估，用于临床决策

Microbiological Rapid On-Site Evaluation for Pulmonary Infectious Diseases

The prompt initiation of empirical anti-infective therapy is crucial in patients presenting with unexplained pulmonary infection. Although imaging acquisition is relatively straightforward in clinical practice, its lack of specificity often necessitates additional time-consuming tests such as sputum culture, bronchoalveolar-lavage fluid culture, or genetic sequencing to identify the underlying etiology of the disease accurately. Moreover, the limited efficacy of empirical anti-infective treatment may contribute to antibiotic misuse. Recent advancements in interpreting microbial background on rapid on-site evaluation (ROSE) slides have enabled clinicians to promptly obtain samples through bronchoscopy (e.g., alveolar lavage, mucosal brushing, tissue clamp), facilitating bedside staining and interpretation that provides essential microbial background information. Consequently, this establishes a foundation for developing targeted anti-infection treatment and individualized drug therapy plans. With a better understanding of which pathogens are causing infections in real-time, physicians can avoid unnecessary broad-spectrum antibiotics contributing to antibiotic resistance. Establishing a rapid and standardized M-ROSE workflow within respiratory medicine departments or intensive care units will greatly assist physicians in formulating accurate treatment strategies for patients, which holds significant clinical implications.

The protocol here demonstrates a fast and standardized microbiological rapid on-site evaluation (M-ROSE) workflow, including three steps: slide making, staining, and interpretation. This protocol will help physicians make rapid clinical decisions.