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hospital of chengdu university of traditional chinese medicine

23 ARTICLES PUBLISHED IN JoVE

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Behavior

Moxibustion Strategies for Mice
Xiao-qin Dai *1,2, Peng Lv *3, Rui-zhen Huang *4, Han-xiao Zhang 3, Si-rui Lin 5, Gang Shi 4, Cheng-shun Zhang 3, Chuan-yi Zuo 6
1Department of Traditional Chinese Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 2Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, 3Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 4Department of Pharmacy Service, Hospital of Chengdu University of Traditional Chinese Medicine, 5Department of Acupuncture, Southwest Medical University Affiliated Traditional Chinese Medicine Hospital, 6Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital

The present protocol describes a moxibustion application method for mice, with the benefits of reducing mouse fearfulness, guaranteeing their welfare, and improving efficiency for researchers.

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Biochemistry

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates
Siyu Guo 1, Zhao Liu 1, Yongshuai Yang 1, Jun Chen 1, Chun Loong Ho 1
1Department of Biomedical Engineering, Southern University of Science and Technology

Here, we describe the use of inducer gradient plates to evaluate bacterial swarming motility while simultaneously obtaining multiple concentration responses.

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Medicine

Acupuncture as a Therapeutic Intervention for Acute Gastrointestinal Injury (AGI): A Preliminary Study
Peng Ding *1, Yuan Zhou *2, Xiujuan Zhou *1, Weiting Sun 1, Peiyang Gao 1
1Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of Geriatrics, The General Hospital of Western Theater Command

Acute gastrointestinal injury (AGI) is a prevalent occurrence in intensive care units and has been linked to unfavorable patient outcomes. The current approaches focus on addressing the primary disease and providing symptomatic relief. The protocol here demonstrates the implementation of acupuncture as a therapeutic intervention for AGI.

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Medicine

Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis
Shi Wang 1,2, Tong Zhou 1,2, Kunlan Long 1, Jun Chen 1, Xiujuan Zhou 1, Peiyang Gao 1
1Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Clinical Medicine Department, Chengdu University of Traditional Chinese Medicine

An enema is a type of traditional Chinese medicine (TCM) therapy used to treat severe acute pancreatitis (SAP). The therapy involves instilling a TCM liquid into the rectum and colon through the anus to keep the liquid in the intestine, absorbing through the intestinal mucosa. This study aims to standardize the enema procedure for SAP patients.

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Medicine

Acupoint Needle-Embedding Combined with Ironing Therapy for Postoperative Pain After Anal Surgery
Xiao Tang 1,2, Jian Kang 1, Ya Liu 1
1Hospital of Chengdu University of Traditional Chinese Medicine, 2Chengdu University of Traditional Chinese Medicine

The high incidence of pain after anal surgery is due to the physiological structure and pathological status of the anus. At present, the use of painkillers cannot provide satisfactory pain relief, which makes complementary therapy important. This paper proposes acupoint needle-embedding combined with ironing therapy to relieve postoperative pain.

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Medicine

Acupoint Application Combined with Acupoint Massage for Treating Constipation in a Patient with Chronic Obstructive Pulmonary Disease
Fanyi Yuan *1, Feifei Lu *1, Yaoyao Guo *1, Chuantao Zhang 1
1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine

Here we demonstrate acupoint application combined with acupoint massage for treating constipation in a patient with chronic obstructive pulmonary disease (COPD).

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JoVE Core

A Traditional Chinese Medicine Characteristic Therapy for Bronchial Asthma: Moxibustion
Weiting Sun *1, Ke Liu *2, Ren Yang *3, Liuxue Guo 1, Peiyang Gao 3
1Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 3Hospital of Chengdu University of Traditional Chinese Medicine

This protocol presents a curative procedure of moxibustion in treating patients with bronchial asthma.

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Medicine

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
Haozhou Huang *1,2, Mengqi Li *3, Chuanhong Luo 4, Sanhu Fan 5, Taigang Mo 5, Li Han 4, Dingkun Zhang 4, Junzhi Lin 6
1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine, 3Sichuan Nursing Vocational College, 4State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 5Sanajon Pharmaceutical Group, 6TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine

Here, we introduce a stability evaluation method based on multiple light scattering technology to evaluate the stability of traditional Chinese medicine extracts.

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Chemistry

An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
Hui Ye 1, Xuemei Liu 1, JiaBao Liao 2, Haozhou Huang 3, Lin Huang 1, Yang Bao 1, Hongyan Ma 1, Junzhi Lin 4, Xiaoming Bao 5, Dingkun Zhang 1, Runchun Xu 1
1State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy School, Chengdu University of Traditional Chinese Medicine, 2China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co., Ltd, 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, 5Shimadzu (China) Co., Ltd

Here, a headspace gas chromatography-tandem quadrupole mass spectrometry (HS-GC-MS/MS) method suitable for the determination of trimethylamine (TMA) in animal-derived medicines is described. The protocol includes sample pretreatment, headspace treatment, analysis conditions, methodological validation, and the determination of TMA in animal-derived medicines.

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Medicine

Auricular Acupuncture as a Traditional Chinese Medicine Therapy for Chronic Obstructive Pulmonary Disease Combined with Sleep Disorders
Yijia Xia *1, Tianbao Wang *2, Zhipeng Ma 1, Rongtao Ying 1, Shiliang Shao 1, Jurong Zeng 1, Chuantao Zhang 1
1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2General Department, Sichuan Cancer Hospital

This protocol introduces an effective method to improve the sleep condition of patients with chronic obstructive pulmonary disease and alleviate cough symptoms by using auricular acupuncture techniques to percutaneously stimulate specific acupoints in the patients' ears.

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Medicine

A Preliminary Study on Warm Acupuncture and Moxibustion for Treating Chronic Obstructive Pulmonary Disease with Abdominal Distension
Shishuang Yu *1, Tianbao Wang *2, Rongtao Ying 1, Yi Zhu 1, Ke Liu 1, Fanyi Yuan 1, Hongjing Yang 1, Keling Chen 1, Jing Shi 3, Xialing Luo 1
1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of General Diseases, Sichuan Cancer Hospital, 3Department of Nursing, Hospital of Chengdu University of Traditional Chinese Medicine

Here, we present a protocol to properly manipulate warm acupuncture and moxibustion for treating chronic obstructive pulmonary disease (COPD) with abdominal distension.

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Medicine

Effects of Mindfulness Training Combined with Tai Chi in Patients with Diabetic Peripheral Neuropathy
Yan Yue *1, Min Deng *1, Shunqi Liao 1, Guojin Xiao 1, Yan Huang 1
1Hospital of Chengdu University of Traditional Chinese Medicine

When studying exercise for treating diabetic peripheral neuropathy (DPN), achieving mind-body exercise is essential. This study proposes a protocol for mindfulness training combined with Tai Chi to achieve mind-body exercise.

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Medicine

Ear Plaster Therapy as a Safe and Effective Treatment for Gestational Vomiting
Shiliang Shao 1, Wenjuan Zheng 1, Yi Wen 1
1Hospital of Chengdu University of Traditional Chinese Medicine

The presented protocol describes a traditional Chinese medicine treatment procedure known for its safety and effectiveness in managing gestational vomiting.

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Medicine

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model
Junjie Li 1, Yuchen Hu 1, Haoming You 1, Ruxu Li 1, Qiang Ran 1, Tianxin Ouyang 1, Yong Huang 2
1Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese Medicine

This protocol presents an economical and efficient method for quantitative evaluation of bone microarchitecture in a mouse model of osteoporosis by combining Hematoxylin-Eosin (HE) staining and micro-computed tomography (Micro-CT) techniques.

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Medicine

Immunofluorescent Labeling in Nasal Mucosa Tissue Sections of Allergic Rhinitis Rats via Multicolor Immunoassay
Yucheng Hu *1, Hongping Luo *1, Junjie Li 1, Rong Zhang 1, Linyou Fu 1, Qiuyi Ren 1, Yue Chen 1, Xueting Huang 1, Zhihang Zhou 1, Hao Yuan 1, Li Tian 1, Xiaobo Wang 2
1Hospital of Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine

This protocol describes a multicolor immunofluorescence technique for evaluating the rat model of allergic rhinitis.

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Medicine

Electroacupuncture Combined with Chinese Medicine Ironing Therapy for Functional Constipation
Liuying Li 1, Hui Yan 2, Jianzhi Cao 2, Fengya Zhu 2, Peimin Feng 1, Xia Zhou 2
1Hospital of Chengdu University of Traditional Chinese Medicine, 2Traditional Chinese Medicine Department, Zigong First People's Hospital

Electroacupuncture combined with Chinese medicine ironing therapy can effectively improve patients' constipation symptoms and their quality of life. Here, we present a protocol to describe the treatment process of electroacupuncture combined with Chinese medicine ironing therapy for functional constipation.

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Medicine

Microbiological Rapid On-Site Evaluation for Pulmonary Infectious Diseases
Xiangwen Weng *1, Weiting Sun *2, Zhenchun Luo *1, Yuling Zhou 1, Xing An 3
1Department of Emergency and the Intensive Care Unit, Chongqing Hospital of Traditional Chinese Medicine, 2Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 3Respiratory and critical care, Chongqing Hospital of Traditional Chinese Medicine

The protocol here demonstrates a fast and standardized microbiological rapid on-site evaluation (M-ROSE) workflow, including three steps: slide making, staining, and interpretation. This protocol will help physicians make rapid clinical decisions.

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Medicine

Acupoint Catgut Embedding for Treatment of Chronic Pelvic Pain Due to the Sequelae of Pelvic Inflammatory Disease
Wei Yang 1, Hui-lin Liu 1, Shan-shan Lu 1, Pei-xuan Fan 1, Wen Yi 1, Xiao-li Ji 1
1Hospital of Chengdu University of Traditional Chinese Medicine

This article outlines acupoint catgut embedding therapy for chronic pelvic pain caused by the sequelae of pelvic inflammatory diseases.

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Medicine

Press Needle: A Traditional Chinese Medicine Therapy for Myopia Patients with Dry Eye
Tianyao Gong *1,2, Qun Huang *3, Haiyan Zhang *1, Chunmeng Liu 1, Xiaojuan Su 1, Yiqi Yang 2, Xinglin Liu 2, Yanlin Zheng 3, Hejiang Ye 3
1Eye School of Chengdu University of Traditional Chinese Medicine, 2Chengdu University of Traditional Chinese Medicine, 3Hospital of Chengdu University of Traditional Chinese Medicine

Here, we present a protocol to standardize the operation of acupuncture in the treatment of myopia with dry eye.

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Medicine

Acupoint Application Combined with Ear Plaster Therapy for Treating Sleep Disorders with Acute Exacerbation of Chronic Obstructive Pulmonary Disease
Hualing Wang *1, Li Liu *2, Chunyi Wang 3, Fanyi Yuan 1, Yufei Liu 1, Feifei Yang 1, Cuifang He 1, Yuanyue Tang 4, Tianbao Wang 5
1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of Pharmacy, Hospital of Chengdu University of Traditional Chinese Medicine, 3Clinical Medical College, Chengdu University of Traditional Chinese Medicine, 4Nursing Department, Hospital of Chengdu University of Traditional Chinese Medicine, 5Outpatient Department, Sichuan Cancer Hospital

The present protocol describes the specific process and efficacy of the acupoint application combined with ear plaster therapy for sleep disorders in a patient with acute exacerbation of chronic obstructive pulmonary disease, including medical devices, selection of acupoints, operating methods, and posttreatment care.

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Medicine

Spinal Hernia Repair and Cauda Equina Repositioning after Lumbar Decompression under Three-Dimensional Microscopy: A Case Report and Literature Review
Zhenhao Zhang 1, YiBo Dong 2, YiZhou Xie 2, HanXiang Yang 2, Xiaohong Fan 2, Yang Yu 2
1Traditional Chinese Medicine Orthopedics and Traumatology, Guangxi University of Chinese Medicine, 2Traditional Chinese Medicine Orthopedics and Traumatology, Hospital of Chengdu University of Traditional Chinese Medicine

Dural hernia following spinal endoscopic surgery is a rare complication. Here, we report a case of nerve root hernia associated with the absence of dural repair, prolonged operation time, and increased abdominal pressure due to postoperative constipation. Early dural tear repair and monitoring postoperative constipation can help prevent cauda equina herniation.

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Medicine

Sampling of N-methyl-N-nitrosourea-induced Rat's Retina Damage and Multi-index Evaluation of Pathological Changes
Yafei Zhu *1, Xinlin Zhao *1, Tibenda Jonnea Japhet 1, Jun Chen 2, Qipeng Zhao 1
1School of Nursing, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, 2Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine

This protocol describes a procedure for rat retinal sampling and utilizing hematoxylin-eosin staining, TUNEL assay, and Western blot to detect pathological changes and apoptosis in the retina after intraperitoneal injection of N-Methyl-N-Nitrosourea.

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Medicine

Alternative Therapy for Acute Exacerbation of Chronic Obstructive Pulmonary Disease: Moving Cupping Along Meridians
Ke Chen *1,2, Yijia Xia *1, Ke Liu 1, Tianbao Wang 3, Chuantao Zhang 1
1Hospital of Chengdu University of Traditional Chinese Medicine, 2People's Hospital of Xinjin District, 3Outpatient Department, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, Affiliated Cancer Hospital of University of Electronic Science and Technology of China

This protocol presents a novel approach to alleviating the symptoms and improving the quality of life of patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD) by employing moving cupping along meridians therapy to stimulate the first sideline of the bladder meridian.

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