Name: an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery
Uploaded: 2011-05-11T12:00:00
Description: Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

The authors would like to thank Dr., Robert Bloch for his generous donation of laboratory space and facilities and Dr. Rao Gullapalli and Da Shi in the Core for Translational Imaging at Maryland (C-TRIM) and the Magnetic Resonance Research Center (MRRC) for technical support. This work was supported by grants to RML from the National Institutes of Health (K01AR053235 and 1R01AR059179) and from the Muscular Dystrophy Association (#4278), and by a grant to JAR from the Jain Foundation.

1. in vivo injury Model and measurement of isometric torque.
<ol>
 <li> These procedures can be used for rats or mice7,17,18. To begin, place the animal supine under inhalation anesthesia (~ 4-5% isoflurane 
 for induction in an induction chamber,then ~ 2% isoflurane via a nosecone for maintenance) using a precision vaporizer (cat # 91103, Vet Equip, Inc, 
 Pleasanton, CA). Apply sterile ophthalmic cream (Paralube Vet Ointment, PharmaDerm, Floham Park, NJ) to each eye to protect the corneas ...

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"Muscle damage" has been defined and measured in many ways. Structural damage is evident in histological findings6,9, but one problem with many of the biological markers used to assess muscle injury, including those used in animal studies, is that they usually do not correlate with the loss of force. Muscle damage is often defined within the context of the assay used to examine it and no one finding can account for the changes in contractility after injury. Since full contractile function can persist...

(All equipment is the same for mice and rats except for the footplate)
<ul>
	<li>BUD Value Line Cabinet (Newark, 06M4718)</li>
	<li>Multifunction l/O USB-6221M (National Instruments, 779808-01)</li>
	<li>Stepper motor controller (Newark, 16M4189)</li>
	<li>Stepper Motor (Newark, 16M4198)</li>
	<li>Strain Gauge Amplifier (Honeywell, Sensotec, DV-05)</li>
	<li>Torque Sensor (Honeywell, QWLC-8M)</li>
	<li>Foot plate and stabilization device (custom made, patent pending)</li>
</ul>

an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery

Muscle strains are one of the most common complaints treated by physicians. A muscle injury is typically diagnosed from the patient history and physical exam alone, however the clinical presentation can vary greatly depending on the extent of injury, the patient's pain tolerance, etc. In patients with muscle injury or muscle disease, assessment of muscle damage is typically limited to clinical signs, such as tenderness, strength, range of motion, and more recently, imaging studies. Biological markers, such as serum creatine kinase levels, are typically elevated with muscle injury, but their levels do not always correlate with the loss of force production. This is even true of histological findings from animals, which provide a "direct measure" of damage, but do not account for all the loss of function. Some have argued that the most comprehensive measure of the overall health of the muscle in contractile force. Because muscle injury is a random event that occurs under a variety of biomechanical conditions, it is difficult to study. Here, we describe an in vivo animal model to measure torque and to produce a reliable muscle injury. We also describe our model for measurement of force from an isolated muscle in situ. Furthermore, we describe our small animal MRI procedure.

Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

An in vivo animal model of injury is described. The method takes advantage of the subcutaneous position of the fibular nerve. Velocity, timing of muscle activation, and arc of motion are all pre-determined and synchronized using commercial software. Post injury changes are monitored in vivo using MR imaging/spectroscopy.

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Muscle strains are one of the most  common complaints treated by physicians. A muscle injury is typically diagnosed from the patient history and  physical ...

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