卡瓦略医生的工作是对妇女的健康和国立儿童健康和人类发展的美国国立卫生研究院（5K12 HD043452）研究办公室的研究经费支持。焦虑医生收到I-流量（加利福尼亚州Lake Forest）的电源（ON-Q PainBuster术后疼痛缓解系统）和资金的生化检测。

1。研究协议<ul><li>获得机构审查委员会的批准，并签署知情同意书。 </li><li>选择适当的手术人群研究的行为。这种方法只适用于剖宫产的伤口，但大多数外科伤口将是适当的。 </li><li>考虑您的参与研究的纳入和排除标准。病人和外科伤口感染率高的程序，可能不适合。 </li><li>规范麻醉及术后管理，对所有患者，以避免潜在的混杂因素。 </li></ul> 2。伤害性刺激和炎症的生化调解收藏<ul><li>到皮下组织层，将ON-Q SilverSoaker（I-流，森林湖，CA）的ON-Q PainBuster的前伤口愈合的其他组织层（可以考虑）。 ON-Q PainBuster疼痛救助制度不断提供生理盐水2毫升/小时的速度。载体可以是梳状独立非执行董事或作为局部麻醉剂和非甾体类消炎药等药物。 </li><li>进入这个系统包括一个标准的三通阀，允许愿望，伤口渗出液中，在指定的时间点。连续输注凝血防止导管，并提高了系统的可靠性，以产生渗出液样品。如果渗出物的愿望是困难的（约5％的情况下），轻轻推上面的伤口，改变对象的位置（ 例如 ，坐或卧的病人他们平），用0.5-1毫升生理盐水冲洗，或者撤销导管1-2厘米。 </li><li>在协议规定的时间点（ 例如 ，1，4，6，24，和48小时后，剖宫产），吸撤出伤口渗出液1毫升。渗出液转移到含有30μl的蛋白酶抑制剂的聚乙烯杯中。我们的目的，我们使用完整的蛋白酶抑制剂（罗氏生物科学，帕洛阿尔托，加利福尼亚州）。 </li><li在相同的时间间隔的研究协议规定，收集10毫升血液。我们已经使用了含有肝素锂的血液收集管，在我们的机构，这是一个绿色的平顶的玻璃管。添加一个额外的300微升蛋白酶抑制剂进入血液样本。 </li><li>收集在1小时内，把样品在冰和离心机以3,000 rpm离心10分钟。 </li><li>删除血清和伤口的上清液和地方在一个标准的离心管中，储存在-20°C </li></ul> 3。含量分析<ul><li>一旦所有的研究渗出液和血清样品的收集和存储，解冻和分析的样品在相同的时间。 </li><li>我们测量了使用多重免疫细胞因子和趋化因子。多重免疫检测技术，使我们能够分析物分析众多小体积样品（50微升）。复用产生的结果与那些获得与ELISA。我们已经使用了17复杂生物PlexTM的系统（BioRad公司，Herc并且ULES，CA）为我们分析。此测定是能够测量的肿瘤坏死因子α（TNFα），干扰素α（INFα），白细胞介素1β（IL-1β），白细胞介素2（IL-2），白细胞介素4（IL-4），白细胞介素5（IL-5。 ），白细胞介素6（IL-6），白细胞介素7（IL-7），白细胞介素8（IL-8），白细胞介素（IL-10），白细胞介素12（IL-12），白细胞介素13（IL-13），白细胞介素17（IL-17），粒细胞集落刺激因子（G-CSF），粒细胞 - 巨噬细胞集落刺激因子（GM-CSF），单核细胞趋化蛋白1（MCP-1）和巨噬细胞炎性蛋白-1（MIP-1β）。神经生长因子与NGF DY256抗体测定用的Bio-Plex的胺偶联试剂盒，通过将其添加到17复杂的板。 </li><li>为了保证良好的质量分析，每次测量应一式两份。每个分析物的标准曲线所产生的使用由制造商提供的参考分析物（浓度为0.20，0.78，3.13，12.5，50，200，800，3200皮克/毫升加零标准生理盐水）。标准曲线包括在每次运行中，样品浓度的计算生物-Plex的Manager软件。 </li><li> P物质和前列腺素E2的测定采用高灵敏度ELISA试剂盒。我们已经使用含量设计公司（密歇根州Ann Arbor）这一分析。下进行分析，一式两份，根据制造商的说明书。 </li></ul>

<ol>
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没有利益冲突的声明。

上文所述的方法，被认为是敏感，可以探测生化伤口渗出液中的炎症和疼痛介质。在不同时间点的采样也让我们能够观察到这些介质的变化。1,2随着时间的推移，我们不知道任何其他人的手术伤口分析，可以连续测量手术伤口渗出液中的炎症和疼痛介质的释放曲线。从我们的方法从手术切口部位的伤口渗出液收集的结果是不同研究利用水渠从髋关节，7,8这表明，皮下伤口关节内或...

手术切口后，居住和迁移炎症细胞释放的亲和抗炎性细胞因子，前列腺素，神经肽和趋化因子是重要的起始和维持疼痛。3,4收集站点特定的生化标志物的能力是至关重要的决定血清及手术伤口的水平之间的关系，了解介质的释放和手术的痛苦之间的复杂关系，并能够手术伤口生物化学的效果进行评估的全身和周边药品监督管理局。研究都集中在全身的生化介质的测量，并没有考虑特定地点的释放。5,6血清细胞因子和趋化因子的测量结果可能并不能反映局部手术伤口的生化事件，只能说明一个全球性的全身反应，手术损伤。本文提供了一个详细的和独特的视觉描述，这种方法是杉木ST在2008年引用的疼痛期刊。此朱庇特的文章的目的是提供一个独特的视觉效果的描述方法。

collecting and measuring wound exudate biochemical mediators in surgical wounds

我们描述了一种方法，使我们能够收集并测量生化在手术伤口部位的炎症和疼痛介质。收集站点特定的生化标志物，重要的是要理解之间的关系血清及手术伤口的水平，确定任何介质的释放，疼痛，止痛药使用和其他成果之间的关联，并评估全身和周边药品监督管理局对手术伤口的效果生物化学。这种方法已被应用于健康与脊髓麻醉的择期行剖宫产的妇女。我们测量在相同的时间间隔为病人的疼痛评分和止痛剂的消费量高达48小时后剖宫产的伤口渗出液和血清介质。使用这种方法，我们已经能够检测各种生化介质，包括神经生长因子（NGF），前列腺素E2（PG-E2），P物质，IL-1β，IL-2，IL-4，IL-6，IL-7， IL-8，IL-10，IL-12，IL-13，IL-17，TNFα，INFγ，G-CSF，GM-CSF，MCP-1和MIP-1β。应用此人的手术伤口生物测定的研究都没有发现之间的相关性伤口和血清细胞因子或释放（J疼痛，2008，9（7）:650-7）。我们也记录了实用的技术，识别药物介导，伤口细胞因子含量（2010年麻醉; 111:1452-9）。

使用这种方法，我们已经能够检测到所有的生化介质上面概述与伤口渗出液和血浆中的IL-5的异常。1,2一些额外的血浆中未检测到细胞因子（IL-4，IL-17，TNFα，INFγ的，GM-CSF）的时间过程中测量的细胞因子和趋化因子的研究报道是一致的，达到了高原的前几个小时内，持续24小时的观察期内1,2然而，NGF稳步增长24小时采集周期，而PGE2在1小时内达到高峰，然后逐步下降。特别值得一提?...

Watch this Scientific Journal Video about Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds at JoVE.com

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds

本文提供了一个详细和视觉的描述的生化在剖宫产手术伤口部位的炎症和疼痛介质收集和测量方法的。已被用于这种人类生物测定，以确定伤口和血清中的细胞因子的浓度和伤口的细胞因子，趋化因子和神经肽的药物介导的变化之间的相关性。

手术伤口伤口渗出液生化调解员在收集和测量

We describe a methodology by which we are able to collect and measure biochemical inflammatory and nociceptive mediators at the surgical wound site. ...

collecting-measuring-wound-exudate-biochemical-mediators-surgical

Research

JoVE Journal

Medicine

11.9K 次观看.  Stanford University School of Medicine. 本文提供了一个详细和视觉的描述的生化在剖宫产手术伤口部位的炎症和疼痛介质收集和测量方法的。已被用于这种人类生物测定，以确定伤口和血清中的细胞因子的浓度和伤口的细胞因子，趋化因子和神经肽的药物介导的变化之间的相关性。

视频: Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds

Biochemical Measurement of Neonatal Hypoxia

Neonatal hypoxia ischemia is characterized by inadequate blood perfusion of a tissue or a systemic lack of oxygen. This condition is thought to cause/exacerbate well documented neonatal disorders including neurological impairment 1-3. Decreased adenosine triphosphate production occurs due to a lack of oxidative phosphorylation. To compensate for this energy deprived state molecules containing high energy phosphate bonds are degraded 2. This leads to increased levels of adenosine which is subsequently degraded to inosine, hypoxanthine, xanthine, and finally to uric acid. The final two steps in this degradation process are performed by xanthine oxidoreductase. This enzyme exists in the form of xanthine dehydrogenase under normoxic conditions but is converted to xanthine oxidase (XO) under hypoxia-reperfusion circumstances 4, 5. Unlike xanthine dehydrogenase, XO generates hydrogen peroxide as a byproduct of purine degradation 4, 6. This hydrogen peroxide in combination with other reactive oxygen species (ROS) produced during hypoxia, oxidizes uric acid to form allantoin and reacts with lipid membranes to generate malondialdehyde (MDA) 7-9. Most mammals, humans exempted, possess the enzyme uricase, which converts uric acid to allantoin. In humans, however, allantoin can only be formed by ROS-mediated oxidation of uric acid. Because of this, allantoin is considered to be a marker of oxidative stress in humans, but not in the mammals that have uricase.
We describe methods employing high pressure liquid chromatography (HPLC) and gas chromatography mass spectrometry (GCMS) to measure biochemical markers of neonatal hypoxia ischemia. Human blood is used for most tests. Animal blood may also be used while recognizing the potential for uricase-generated allantoin. Purine metabolites were linked to hypoxia as early as 1963 and the reliability of hypoxanthine, xanthine, and uric acid as biochemical indicators of neonatal hypoxia was validated by several investigators 10-13. The HPLC method used for the quantification of purine compounds is fast, reliable, and reproducible. The GC/MS method used for the quantification of allantoin, a relatively new marker of oxidative stress, was adapted from Gruber et al 7. This method avoids certain artifacts and requires low volumes of sample. Methods used for synthesis of MMDA were described elsewhere 14, 15. GC/MS based quantification of MDA was adapted from Paroni et al. and Cighetti et al. 16, 17. Xanthine oxidase activity was measured by HPLC by quantifying the conversion of pterin to isoxanthopterin 18. This approach proved to be sufficiently sensitive and reproducible.

A method is described to measure biochemical markers of neonatal hypoxia-ischemia. The approach utilizes high pressure liquid chromatography (HPLC) and Gas Chromatography Mass Spectrometry (GC/MS).

新生儿缺氧的生化测量

Neonatal hypoxia ischemia is characterized by inadequate blood perfusion of a tissue or a systemic lack of oxygen. This condition is thought to ...

科研

医学

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Salivary measures have emerged in bio-behavioral research that are easy-to-collect, minimally invasive, and relatively inexpensive biologic markers of stress. This article we present the steps for collection and analysis of two salivary assays in research with frail, community residing older adults-salivary cortisol and salivary alpha amylase. The field of salivary bioscience is rapidly advancing and the purpose of this presentation is to provide an update on the developments for investigators interested in integrating these measures into research on aging. Strategies are presented for instructing family caregivers in collecting saliva in the home, and for conducting laboratory analyses of salivary analytes that have demonstrated feasibility, high compliance, and yield quality specimens. The protocol for sample collection includes: (1) consistent use of collection materials; (2) standardized methods that promote adherence and minimize subject burden; and (3) procedures for controlling certain confounding agents. We also provide strategies for laboratory analyses include: (1) saliva handling and processing; (2) salivary cortisol and salivary alpha amylase assay procedures; and (3) analytic considerations.

We demonstrate: (1) procedures for collection of salivary samples in cognitive impaired older adults by family caregivers in the home setting, (2) procedures for measuring stress activity via salivary cortisol and alpha amylase, and (3) representative profiles. Protocols that allow researchers to study stress-linked processes advance our understanding of biological sensitivity and susceptibility.

Salivary measures have emerged in bio-behavioral research  that are easy-to-collect, minimally invasive, and relatively inexpensive  biologic markers of ...

In utero Measurement of Heart Rate in Mouse by Noninvasive M-mode Echocardiography

Congenital heart disease (CHD) is the most frequent noninfectious cause of death at birth. The incidence of CHD ranges from 4 to 50/1,000 births (Disease and injury regional estimates, World Health Organization, 2004). Surgeries that often compromise the quality of life are required to correct heart defects, reminding us of the importance of finding the causes of CHD. Mutant mouse models and live imaging technology have become essential tools to study the etiology of this disease. Although advanced methods allow live imaging of abnormal hearts in embryos, the physiological and hemodynamic states of the latter are often compromised due to surgical and/or lengthy procedures. Noninvasive ultrasound imaging, however, can be used without surgically exposing the embryos, thereby maintaining their physiology. Herein, we use simple M-mode ultrasound to assess heart rates of embryos at E18.5 in utero. The detection of abnormal heart rates is indeed a good indicator of dysfunction of the heart and thus constitutes a first step in the identification of developmental defects that may lead to heart failure.

In utero Measurement of Heart Rate in Mouse by Noninvasive M-mode Echocardiography

Ultra-high frequency ultrasound is a powerful live imaging tool to examine cardiac abnormalities in small animals. Its noninvasiveness allows maintaining the physiologic state of embryos. Herein, we show the use of M-mode ultrasound to measure the heart rates of embryos at E18.5 in utero.

心率在小鼠中通过无创M型超声心动图测量子宫内

Congenital heart disease (CHD) is the most frequent noninfectious cause of death at birth. The incidence of CHD ranges from 4 to 50/1,000 births (Disease ...

Demonstration of the Rat Ischemic Skin Wound Model

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and unrelieved pressure due to immobility. Animal models have been developed that attempt to mimic these conditions for the purpose of furthering our understanding of the complexity of chronic wounds. The model described herein is a rat ischemic skin flap model that permits a prolonged reduction of blood flow resulting in wounds that become ischemic and resemble a chronic wound phenotype (reduced vascularization, increased inflammation and delayed wound closure). It consists of a bipedicled dorsal flap with 2 ischemic wounds placed centrally and 2 non-ischemic wounds lateral to the flap as controls. A novel addition to this ischemic skin flap model is the placement of a silicone sheet beneath the flap that functions as a barrier and a splint to prevent revascularization and reduce contraction as the wounds heal. Despite the debate of using rats for wound healing studies due to their quite distinct anatomic and physiologic differences compared to humans (i.e., the presence of a panniculus carnosus muscle, short life-span, increased number of hair follicles, and their ability to heal infected wounds) the modifications employed in this model make it a valuable alternative to previously developed ischemic skin flap models.

The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.

在大鼠缺血性皮肤创伤模型的演示

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and ...

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

Conventional imaging techniques can provide detailed information about cellular processes. However, this information is based on static images in an otherwise dynamic system, and successive phases are easily overlooked or misinterpreted. Live-cell imaging and time-lapse microscopy, in which living cells can be followed for hours or even days in a more or less continuous fashion, are therefore very informative. The protocol described here allows for the investigation of the fate of chemotherapeutic nanoparticles after the delivery of doxorubicin (dox) in living cells. Dox is an intercalating agent that must be released from its nanocarrier to become biologically active. In spite of its clinical registration for more than two decades, its uptake, breakdown, and drug release are still not fully understood. This article explores the hypothesis that lipid-based nanoparticles are taken up by the tumor cells and are slowly degraded. Released dox is then translocated to the nucleus. To prevent fixation artifacts, live-cell imaging and time-lapse microscopy, described in this experimental procedure, can be applied.

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

Live-cell imaging is a powerful tool to visualize dynamic processes. The examination of fixed cells provides only static pictures, which can lead to misinterpretation and confusion about the process. This work presents a method to study uptake, drug release, and intracellular localization of liposomal nanoparticles in living cells.

使用体外活体细胞成像技术研究脂纳米粒子化疗

Conventional imaging techniques can provide detailed information about cellular processes. However, this information is based on static images in an ...

Collecting Hair Samples for Hair Cortisol Analysis in African Americans

The hormone cortisol is typically assessed in saliva, serum, or urine samples. More recently, cortisol has been successfully extracted from hair, including humans. The advantage of hair cortisol concentration is that it reflects a retrospective representation of hypothalamic-pituitary-adrenal (HPA) axis function over time, much like hemoglobin A1C represents glycemic control. However, obtaining hair samples can be challenging, due to the cultural beliefs and hair care practices of minority participants. For example, African Americans may be reluctant to provide samples. Additionally, few researchers are trained to collect hair samples from African Americans. The purpose of this paper is to present a culturally informed protocol to help researchers obtain hair samples from African Americans. To illustrate the representative results of this protocol implementation, de-identified data from African Americans that participated in a community-based study on chronic stress are provided. Hair practice preferences are assessed. The participants are made comfortable by showing pictures of hair samples prior to cutting their hair. The single strain twist and gently pull method is used to collect approximately 30 - 50 strands of hair from the posterior vertex region of the scalp. This protocol will significantly improve collection of hair samples from African Americans.

Hair cortisol concentration analysis provides an alternative to traditional measures of cortisol; however, to collect hair samples from African Americans, scientists need to be culturally informed and competent. The purpose of this protocol is to demonstrate a culturally informed technique to collect hair samples for cortisol analysis from African Americans.

在非洲裔美国人中收集头发皮质醇分析样本

The hormone cortisol is typically assessed in saliva, serum, or urine samples. More recently, cortisol has been successfully extracted from hair, ...

Protocol to Create Chronic Wounds in Diabetic Mice

Chronic wounds develop as a result of defective regulation in one or more complex cellular and molecular processes involved in proper healing. They impact ~6.5M people and cost ~$40B/year in the US alone. Although a significant effort has been invested in understanding how chronic wounds develop in humans, fundamental questions remain unanswered. Recently, we developed a novel mouse model for diabetic chronic wounds that have many characteristics of human chronic wounds. Using db/db-/- mice, we can generate chronic wounds by inducing high levels of oxidative stress (OS) in the wound tissue immediately after wounding, using a one-time treatment with inhibitors specific to the antioxidant enzymes catalase and glutathione peroxidase. These wounds have high levels of OS, develop biofilm naturally, become fully chronic within 20 days after treatment and can remain open more for more than 60 days. This novel model has many features of diabetic chronic wounds in humans and therefore can contribute significantly to advancing fundamental understanding of how wounds become chronic. This is a major breakthrough because chronic wounds in humans cause significant pain and distress to patients and result in amputation if unresolved. Moreover, these wounds are very expensive and time-consuming to treat, and lead to significant loss of personal income to patients. Advancements in this field of study through the use of our chronic wound model can significantly improve health care for millions who suffer under this debilitating condition. In this protocol, we describe in great detail the procedure to cause acute wounds to become chronic, which has not been done before.

Chronic wounds are developed from acute wounds on a diabetic mouse model by inducing high levels of oxidative stress after a full-thickness cutaneous wound. The wound is treated with inhibitors for catalase and glutathione peroxidase, resulting in impaired healing and biofilm development by bacteria present in the skin microbiome.

在糖尿病小鼠中创建慢性伤口的协议

Chronic wounds develop as a result of defective regulation in one or more complex cellular and molecular processes involved in proper healing. They impact ...

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

The murine cornea provides an excellent model to study wound healing. The cornea is the outermost layer of the eye, and thus is the first defense to injury. In fact, the most common type of eye injury found in clinic is a corneal abrasion. Here, we utilize an ocular burr to induce an abrasion resulting in removal of the corneal epithelium in vivo on anesthetized mice. This method allows for targeted and reproducible epithelial disruption, leaving other areas intact. In addition, we describe the visualization of the abraded epithelium with fluorescein staining and provide concrete advice on how to visualize the abraded cornea. Then, we follow the timeline of wound healing 0, 18, and 72 h after abrasion, until the wound is re-epithelialized. The epithelial abrasion model of corneal injury is ideal for studies on epithelial cell proliferation, migration and re-epithelialization of the corneal layers. However, this method is not optimal to study stromal activation during wound healing, because the ocular burr does not penetrate to the stromal cell layers. This method is also suitable for clinical applications, for example, pre-clinical test of drug effectiveness.

This protocol describes a method to inflict an abrasion to the ocular surface of the mouse, and to follow the wound healing process thereafter. The protocol takes advantage of an ocular burr to partially remove the surface epithelium of the eye in anaesthetized mice.

眼毛刺角膜上皮磨损与角膜创面愈合的模型研究

The murine cornea provides an excellent model to study wound healing. The cornea is the outermost layer of the eye, and thus is the first defense to ...

Targeting Gray Rami Communicantes in Selective Chemical Lumbar Sympathectomy

Chemical lumbar sympathectomy (CLS) is a commonly used, minimally invasive procedure for the treatment of conditions including ischemic diseases of the lower extremities, hyperhidrosis, etc. It is commonly practiced to position the puncture needle tip in front of the anterior fascia of the psoas major muscle and inject the inactivating agent around the sympathetic trunk, which is defined as conventional CLS. Although relatively rare, ureteropelvic damage is the most frequently reported complication of conventional CLS and can cause serious harm to patients. We found that injecting the inactivating agent behind the anterior fascia, which only targets gray rami communicantes, helped achieve therapeutic efficacy in&#160;vasodilation, sweat reduction, and pain relief comparable to conventional CLS, and serious complications were largely reduced. We define this procedure as selective CLS. Here, we present a protocol of selective CLS. The precise needle tract and accurate evaluation of the spreading of the contrast agent are critical to ensure that the drug is injected behind the anterior fascia of the psoas major muscle. The needle tip is at approximately one-third the dividing line of the vertebral body in the lateral view of a lumbar X-ray. The contrast is mainly confined around the needle tip and spreads outward and downward along the psoas muscle fibers. In this way, the anterior fascia provides a natural barrier for the ureteropelvic area, and the psoas major muscle provides a natural barrier for the lumbar nerve root. There are several highlights of this article, including 1) a detailed description of the selective CLS procedures, 2) an explanation of the anatomical basis for the implementation of selective CLS, and 3) an explanation of the differences between selective and conventional CLS.

We present a protocol for selective chemical lumbar sympathectomy (CLS), which inactivates only gray rami communicantes and not the sympathetic trunk. Selective CLS can help achieve therapeutic efficacy in vasodilation, sweat reduction, and pain relief that are comparable to conventional CLS, and serious complications, particularly ureteropelvic damages, can be reduced.

选择性化学腰椎交感神经切除术中灰色拉米关节的靶向性

Chemical lumbar sympathectomy (CLS) is a commonly used, minimally invasive procedure for the treatment of conditions including ischemic diseases of the ...

Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings

Biological therapy using Lucilia sericata larvae has numerous advocates worldwide, yet it is still fairly unknown and not commonly applied in daily practice because of the limited awareness and insufficient experience of medical and nursing personnel. There are case reports suggesting that maggot therapy can be applied and supported by lay caregivers, provided they are supervised and informed by physicians/nurses. The foregoing observation suggests that the method should be considered for implementation by a wider group of caregivers if accepted and meticulously supervised by trained and experienced medical staff. The concerns related to the therapeutic use of maggots in certain regions seem understandable, but are not supported by scientific facts. It should be noted that many therapeutic agents (including brood) used in medicine are of natural origin, and are associated with low production costs and high possibilities of implementation in the course of therapy. By analyzing the literature and using our own clinical and research experience, we have come to conclusions related to using larvae therapy, as a quick and safe method providing cleaning and revitalization in the process of treating wounds of various etiologies, especially pressure ulcers. In the current study, medical-grade Lucilia sericata maggots were applied to remove necrotic tissue from deep pressure sores. The treatment is mostly accepted by both caregivers and patients. In most cases, it is conducted by trained and experienced medical personnel in home and outpatient settings. Over the course of the conducted analyzes involving the collected specimens, there were no statistically significant relationships (p&gt; 0.05) confirmed between the wound surface successfully cleared by brood and variables, such as time from wound formation, location, surface size, and the depth of damage to the tissue structure. The lack of statistical dependence may result from the small size of the studied group. Based on the current findings, we have formulated the following conclusions: Maggot Debridement Therapy (MDT) is a fast and effective method enabling the preparation of the wound bed. The use of MDT in outpatient settings is safe and acceptable for patients and their caregivers.

Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings

Maggots of Lucilia sericata were used in the debridement of deep pressure sores in outpatient settings for the treatment of pressure sores involving the complete skin thickness. The technique presented in the article does not pose risks for the patient.

桫椤幼虫在门诊压力伤口清创中的应用

Biological therapy using Lucilia sericata larvae has numerous advocates worldwide, yet it is still fairly unknown and not commonly applied in daily ...