June 2nd, 2014
•Olfactory receptor activation patterns encode odor identity, but the lack of published data identifying odorant ligands for mammalian olfactory receptors hinders the development of a comprehensive model of odor coding. This protocol describes a method to facilitate high-throughput identification of olfactory receptor ligands and quantification of receptor activation.
Related Videos
Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
In vivo Electroporation of Developing Mouse Retina
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition
Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay