July 13th, 2015
•Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.
Tags
Related Videos
Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis
Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques
Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons