June 6th, 2014
•This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.
Related Videos
Method for Culture of Early Chick Embryos ex vivo (New Culture)
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
A System for ex vivo Culturing of Embryonic Pancreas
A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
Establishment of an Extracellular Acidic pH Culture System