作者承认工作人员在NCDA沙丘研究站与蒸渗仪的安装和折返协助。资金用于代表性的成果描述的实验是由中心的草坪环境研究和教育提供。视频和手稿的生产是由土壤科学和作物科学的北卡罗莱纳州立大学各部门的支持。

野外采样在这个实验中进行，并根据农业和消费者服务的北卡罗来纳部门的授权。 1，现场蒸渗仪的安装<ol><li>选择一个实验性的网站上，其中应用化学横向移动的机会不大（ 即 ，与很少或没有坡度的网站）。选择基础上的利益土壤和植被性质的网站。 </li><li>如果地块植被，植被拔插头式蒸渗仪的安装（ 图1A）之前。 </li><li>向下推动渗计成使用倒置后的驱动程序，留下约1-2厘米的土壤表面以上的蒸散为包含施加化学和减少化学横向移动的需要的图（有或没有植被）。对于这一点，利用焊接而成18轨钢板（91厘米深x 15厘米直径）（ 图1B）。使用不同的材料和尺寸的渗仪，以适应水库目录操作搜索目标。 </li><li>下面蒸渗仪的安装更换植被插头。 </li><li>管理任何植被以适合进行实验。如果图是保持裸露，使用草甘膦点应用，以保持区域提供免费植被。 </li><li>确保灌溉，施肥，以及任何其他管理措施是相同的bareground和植被图。预先灌水，以满足研究目标。 </li></ol> 2，孔隙水采样器安装<ol><li>安装孔隙水采样器，如PTFE /石英（50/50％），中渗仪的中间，收集渗透孔隙水。 </li><li>放置2.5cm的不锈钢棒的蒸散的中心，并将其插入地面用木槌到所需的采样深度。 注意:一个螺旋推运器，也可以用于此步骤。 </li><li>准备一个石英粉和水泥浆用700ml灌溉水来〜900克化学惰性二氧化硅粉。混合浆料thoroug溶血素前各采样被​​放置在该混合物中。从手持或电池供电的真空泵适用-50至-70千帕的压力之间的取样器。 </li><li>从石英粉浆液取出取样器10​​分钟后，彻底再混合二氧化硅浆料。倾60毫升浆料通过连接到2.5厘米直径的管插入孔的底部的漏斗。 </li><li>将取样器中的孔所需的采样深度用塑料或金属管。确保从采样器管道延伸出的洞。使用未处理，原生土壤和水的泥浆回填剩余的孔。 </li><li>回填的土定居在允许的时间;使用管道根据需要添加夯实土壤。 </li><li>土回填到原来的水平。如果合适的话，更换植被在孔的顶部。 </li><li>通过氟化乙烯丙烯（FEP）管节连接采样管连接到一个真空瓶。用塑料管夹，连接第二个管道线出真空瓶与真空泵。 </li><li>涵盖管道和收集瓶的黑色塑料或胶带，如果感兴趣的化学（s）是容易发生光降解（ 图1C）。 </li><li>通过真空瓶重复应用约-50至-70千帕的真空压力的过采样的实验前数天的过程中，以确保适当的采样器的安装。 </li></ol> 3，化学应用渗仪<ol><li>化学应用是由前留出至少两个星期驯化。 </li><li>收集背景孔隙水样品蒸渗仪治疗前量化目标化学（S）的背景浓度。 </li><li>申请所关注的化学品到土壤或植物通过典型的方法，例如用一个手持式CO 2加压喷杆喷雾机（ 图1D），或通过直接的含有蒸散积在表面上分布的颗粒制剂。如果有多个化学应用所必需的功效，适用于他们每个典型使用模式或标签方向。留下一些渗仪未经处理作为对照。 </li></ol> 4，孔隙水收集和分析<ol><li>动用约-50至-70千帕的真空来的前一天或取样当天的孔隙水采样器真空瓶。围绕水采样器将制订通过采样进入管道，流向那里收集到采样的真空瓶。从该孔隙水被收集和水收集时间可以取决于因素如土壤类型，土壤质地，土壤水分含量和采样深度的土壤体积。 </li><li>收集样品在指定的时间间隔以下化学应用中，如预定的研究员。 </li><li>测量所收集的水的体积成量筒每个孔隙水取样。如果过滤是必要的，将水在鲁尔乐学年RINGE（大小将取决于水量），并通过25毫米0.2微米的尼龙过滤器通过样品。 </li><li>如果不同的样品保存方法是必要的，足够的样本采集，将样本分为独特的容器。 </li><li>使用一个手持的pH计，以确定非酸化样品的pH值。 </li><li>通过如果需要的样品保存添加适当的酸的适当量调节pH值。 注:浓酸能腐蚀或氧化剂和护理时，应使用它们服用。 </li><li>冰在一个凉爽的地方样品或放入冰箱直至分析。使用的分析方法为化学计量比如电感耦合等离子体 - 质谱（ICP-MS），电感耦合等离子体 - 光发射光谱（ICP-OES），原子吸收光谱（AAS）或高效液相色谱法（HPLC），以分析样品。 </li></ol> 5，蒸渗仪折返，土壤/植被集合ND分析<ol><li>挖出的渗仪，含土壤和植被，在下面的化学应用指定的时间间隔。发掘未处理渗仪在每个采样时间到土壤和植被中确定的背景化学品浓度。 </li><li>发掘利用连接到一台拖拉机实施桶箍渗仪。放下水桶到一个位置它允许被放置在蒸渗仪的暴露边缘夹。 </li><li>提起实施造成夹钳把握露锋芒，拉动蒸渗仪柱的土壤（ 图1E）。 </li><li>盖挖出蒸渗仪与两端切到渗仪的直径绝缘片。持帽，用插在蒸渗仪两端加仑大小的聚乙烯袋，并用胶带安全袋。 </li><li>运输渗仪到现场实验室进行土壤和植被分样。首先处理未处理渗仪，以防止污染时翁渗仪。 </li><li>使用一个往复锯配备金属切削刀片纵向切开蒸散一侧。从底部（预期低浓度区）顶（预期为高浓度区），以确保土壤在更深的深度不是由土壤在较浅的深度污染削减的列。 </li><li>裂开的蒸渗仪。使用金​​属分隔板分隔离散土壤和植被的部分。选择基础上的蒸渗仪和研究目标的长度土壤深度增量。 </li><li>用勺子或抹刀挖掘的剖面土壤和植被。将每个样品中的适当标记的聚乙烯保鲜袋。不要直接与蒸渗仪接触收集的土壤。 </li><li>按照协议，挖掘每个所需的采样深度。将样品袋在装满冰块冷却器，并将它们运送到实验室。店内样品在冷冻直至分析。 </li></ol>

<ol>
	<li>Wuana, R. A., Okieimen, F. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heavy+Metals+in+Contaminated+Soils:+A+Review+of+Sources,+Chemistry,+Risks+and+Best+Available+Strategies+for+Remediation.">Heavy Metals in Contaminated Soils: A Review of Sources, Chemistry, Risks and Best Available Strategies for Remediation.</a> ISRN Ecology. , 1-20 (2011).</li><li>Donaldson, D., Kiely, T., Wu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> 1-38 U.S. Environmental Protection Agency. , (2011).</li><li>Bergstr&#246;m, L., Bergstr&#246;m, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Environmental+fate+of+chemicals+in+soil.">Environmental fate of chemicals in soil.</a> Ambio. 27, 16-23 (1998).</li><li>Sutton, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Our Nutrient World. The challenge to produce more food &amp; energy with less pollution. Key Messages for Rio +20. , (2012).</li><li>Du, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fate+and+Ecological+Effects+of+Decabromodiphenyl+Ether+in+a+Field+Lysimeter.">Fate and Ecological Effects of Decabromodiphenyl Ether in a Field Lysimeter.</a> Environmental Science and Technology. 47, 9167-9174 (2013).</li><li>Fuhr, F., Burauel, P., Mittelstaedt, W., Putz, T., Wanner, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Environmental fate and effects of pesticides. , 1-29 (2003).</li><li>Hire, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remarques+sur+l'eau+de+la+pluie,+et+sur+l'origine+des+fontaines;+avec+quelues+particularites+sur+la+construction+des+cisternes.+Memoires+de+l'+Academie+Royale.">Remarques sur l'eau de la pluie, et sur l'origine des fontaines; avec quelues particularites sur la construction des cisternes. Memoires de l' Academie Royale.</a> , 56-69 (1703).</li><li>Wagner, G. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+porous+ceramic+cups+to+sample+soil+water+within+the+profile.">Use of porous ceramic cups to sample soil water within the profile.</a> Soil Science. 94, 379-386 (1962).</li><li>Matteson, A. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Arsenic+Retention+in+Foliage+and+Soil+Following+Monosodium+Methyl+Arsenate+(MSMA)+Application+to+Turfgrass.">Arsenic Retention in Foliage and Soil Following Monosodium Methyl Arsenate (MSMA) Application to Turfgrass.</a> Journal of Environmental Quality. 43, 379-388 (2014).</li><li>Sakaliene, O., Papiernik, S. K., Koskinen, W. C., Kavoliunaite, I., Brazenaitei, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+Lysimeters+to+Evaluate+the+Relative+Mobility+and+Plant+Uptake+of+Four+Herbicides+in+a+Rye+Production+System.">Using Lysimeters to Evaluate the Relative Mobility and Plant Uptake of Four Herbicides in a Rye Production System.</a> Journal of Agricultural and Food Chemistry. 57, 1975-1981 (2009).</li><li>Cai, Y., Cabrera, J. C., Georgiadis, M., Jayachandran, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+arsenic+mobility+in+the+soils+of+some+golf+courses+in+South+Florida.">Assessment of arsenic mobility in the soils of some golf courses in South Florida.</a> Science of the Total Environment. 291, 123-134 (2002).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Water+quality,+pesticide+occurrence,+and+effects+of+irrigation+with+reclaimed+water+at+golf+courses+in+Florida.">Water quality, pesticide occurrence, and effects of irrigation with reclaimed water at golf courses in Florida.</a> Swancar, A. (ed USGS) Tallahassee. , (1996).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organic+arsenical+herbicides+(MSMA,+DSMA,+CAMA,+and+Cacodylic+Acid),+reregistration+eligibility+decision;+notice+of+availability.">Organic arsenical herbicides (MSMA, DSMA, CAMA, and Cacodylic Acid), reregistration eligibility decision; notice of availability.</a> Environmental Protection Agency, Federal Register Environmental Documents. , 1-70 (2006).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=EPA+(not+Araujo+as+stated+before)+Organic+Arsenicals;+Amendments+to+Terminate+Uses:+Amendment+to+Existing+Stocks+Provision.">EPA (not Araujo as stated before) Organic Arsenicals; Amendments to Terminate Uses: Amendment to Existing Stocks Provision.</a> Environmental Protection Agency) 18590-18591 Federal Registrar. 78, (2013).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drinking+Water+Regulations;+Arsenic+and+Clarifications+to+Compliance+and+New+Source+Contaminants+Monitoring+Final+Rule.">Drinking Water Regulations; Arsenic and Clarifications to Compliance and New Source Contaminants Monitoring Final Rule.</a> Environmental Protection Agency. 66, (2001).</li><li>Winton, K., Weber, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+field+lysimeter+studies+to+describe+the+environmental+fate+of+pesticides.">A review of field lysimeter studies to describe the environmental fate of pesticides.</a> Weed Technology. 10, 202-209 (1996).</li><li>Bergstr&#246;m, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+lysimeters+to+estimate+leaching+of+pesticides+in+agricultural+soils.">Use of lysimeters to estimate leaching of pesticides in agricultural soils.</a> Environmental Pollution. 67, 325-347 (1990).</li><li>Byron, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lysimeters+promoted+for+pesticide+research.">Lysimeters promoted for pesticide research.</a> Environmental Science and Technology. 31, (1997).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Infographic:+Pesticide+Planet.">Infographic: Pesticide Planet.</a> Science. 341, 730-731 (2013).</li><li>Severson, R., Grigal, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soil+solution+concentrations:+effects+of+extraction+time+using+porous+ceramic+cups+under+constant+tension.">Soil solution concentrations: effects of extraction time using porous ceramic cups under constant tension.</a> Water Resources Bulletin. 12, 1161-1170 (1976).</li><li>Allaire, S. E., Roulier, S., Cessna, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantifying+preferential+flow+in+soils:+A+review+of+different+techniques.">Quantifying preferential flow in soils: A review of different techniques.</a> Journal of Hydrology. 378, 179-204 (2009).</li><li>Weiherm&#252;ller, L., Kasteel, R., Vanderborght, J., P&#252;z, T., Vereecken, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soil+Water+Extraction+with+a+Suction+Cup.">Soil Water Extraction with a Suction Cup.</a> Valdose Zone Journal. 4, 899-907 (2005).</li><li>Jury, W. A., Fluhler, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Advances in Agronomy. 47, 141-201 (1992).</li></ol>

作者什么都没有透露。

利用一个集成领域lysimetry和孔隙水采样方法使研究人员能够评估各种土地应用化学时空分布。化学物质在土壤和植被系统的命运可以通过许多环境过程和属性，例如向下淋溶，挥发，水解，光解，微生物转化/降解，植物吸收，土壤类型，土壤PH值16,17来控制。不像温室或实验室基础实验，从这里所描述的领域为基础的方法的结果与获得对研究的系统影响最小，因此可以外推到其他系统或设...

潜在的有毒化学品的常规运用的来源，如农药，化肥，污水/污泥，工业废弃物和城市垃圾1,2降落。这些化学物质的命运-其可以包括营养物，微量元素，有机物，以及它们相关的代谢物-通常不能很好地理解3。如果化学品管理不当，他们通过他们的转移和积聚于植物，地表水和地下水，威胁人类和环境健康的潜力。与可能达到10十亿人，到2050年全球人口中，有关于废物管理和粮食生产2日益增长的需求，以及土地利用的许多化学物质一直在增加3,4。因此，研究是必要的，量化的变换，流动性，负载限制，以及整体环境风险从需要土地处置或者说我们依靠增强作物健康的化学物质和产量。 许多策略已经被用来评估来自环境中的应用化学品的威胁。实验室为基础，模型系统的研究已进行提供有关控制化学品在土壤中的移动性的基本机制的信息。当分析在实验室化学命运，对"环境"，并输入完整的操作可以实现，但这些很少匹配真实世界的环境条件5,6。因此，实验室结果外推到现场设置可能会导致对化学威胁不准确的预测。与此相反，广泛的现场测量已被用于定义在环境中的化学行为。然而，大约从这些测量环境归趋的结论往往是由于经常使用低速率（ 例如几克-1）应用于化学品，以及在电子水文和生物地球化学过程之间复杂的相互作用复杂nvironment调节化学分布。 Lysimetry，包括现场lysimetry，历来被用于土壤和作物科学系统地评估应用到土壤和植被建立化学品的向下流动。甲渗漏​​测定计是由金属或塑料的一种装置，被放置到感兴趣的土壤和用于确定在已知量施加到一个狭窄区域的化学品的命运。从渗仪收集的土壤和植被的样品可用于评估化学分布随时间的演化。因为字段lysimetry是天然环境的条件下进行的，结果，可用于预测来自于化学应用到土壤系统实例场景。早期的蒸渗仪测量研究蒸腾，水分流动，和/或营养运动。现代蒸渗仪测量研究农药和养分消耗，农药运动，波动性和质量平衡，随着aforementioned测量3。 传统字段lysimetry的一个限制是，在土壤剖面的化学迁移率主要取决于固相的测量所定义，而较少关注到在水中溶解的化学浓度通过土壤渗滤 - 可能影响对地下水污染的可能性的重要组成部分从土地应用的化学品。虽然从渗仪底部渗滤液有时被收集用于分析，孔隙水浓度的这种做法限制深度分辨率，通常需要前实验显著挖土。相反，以获取有关土壤水分的化学品浓度数据，孔隙水采样器可以用在字段设置。孔隙水采样器安装在土壤中，从离散的，所需深度收集水，只有最低限度扰乱土壤系统。孔隙水采样器已经提到了很多名字，包括渗仪，吸痰立方米p渗仪，或土壤溶液取样器，卷积它们之间的区别与上述领域传统的蒸渗仪。在本文中，我们将使用术语"孔隙水采样器"，以减轻混乱。 在这里，我们表明，结合现场lysimetry和孔隙水采样评估应用到植物的土壤或bareground系统化学物质的向下淋失风险的一种实验方法。 Lysimetry一直以来，1700年7采用了功能强大的工具，而陶瓷孔隙水采样已自20世纪60年代初8使用。这些强大的技术整合，可以进行现场测定，同时尽量减少对土壤的扰动两种固体和溶解相的化学浓度分布。本文介绍了设计一个实验，包括选址，设备安装，样品采集时要考虑的因素。该方法与说明，评估一个企业的命运实验有机砷农药施加到bareground和已建立的草坪系统。描述的技术可以根据需要进行调整，研究​​的各种化学品的命运，从而提供宝贵的工具，以研究人员和政策制定者谁寻求理解的土地用于化学品的环境行为和命运。

<table><tbody><tr><td>Prenart Super Quartz Samplers (PFTE/Quartz)</td><td>Prenart Equipment ApS</td><td>N/A</td><td>Any samplers for&amp;nbsp; trace metal analysis can be used (&lt;em&gt;e.g.&lt;/em&gt; SoilMoisture Equipment Corp.)</td></tr><tr><td>Prenart installation kit</td><td>Prenart Equipment ApS</td><td>N/A</td><td>Contains all items necessary to install porewater samplers</td></tr><tr><td>2 L collecting bottles</td><td>Prenart Equipment ApS</td><td></td><td>Bottles can also be purchased from Fisher Scientific (02-923-2) or other laboratory supply companies, but fittings will need to be adjusted. Bottles can be covered with dark material if light sensitive</td></tr><tr><td>Portable vacuum pump</td><td>Prenart Equipment ApS</td><td>N/A</td><td>Vacuporter from Decagon Devices or other field battery-operated or hand vacuum pump may be used</td></tr><tr><td>1 oz HDPE Nalgene bottles</td><td>Fisher Scientific</td><td>03-313-4A</td><td>Sample bottle type will depend on analyte of interest and may be glass</td></tr><tr><td>Concentrated nitric acid</td><td>Fisher Scientific</td><td>A509-P212</td><td>Oxidizing and corrosive-other acids may be needed for preservation and should be used with caution</td></tr><tr><td>25 mm 0.2 &amp;micro;m nylon syringe filters</td><td>VWR</td><td>28145-487</td><td>Other filter types and pore sizes may be used, dependent on the analyte of interest and analytical instrumentation</td></tr><tr><td>60 ml Luer-Lok syringes</td><td>Fisher Scientific</td><td>13-689-8</td><td>Other sizes may be used depending on sample volume collected</td></tr><tr><td>Portable pH meter</td><td>VWR</td><td>248481-A01</td><td>Other pH meters can be used following calibration</td></tr><tr><td>Graduated cylinder</td><td>any</td><td>N/A</td><td></td></tr><tr><td>Field lysimeters (metal, plastic, &lt;em&gt;etc.&lt;/em&gt;)</td><td>N/A</td><td>N/A</td><td>Often these are constructed based on the researchers specifications</td></tr><tr><td>Inverted post driver tractor</td><td>N/A</td><td>N/A</td><td>Any tractor can be used to install the lysimeters</td></tr><tr><td>Handheld boom sprayer</td><td>N/A</td><td>N/A</td><td>To apply the rate needed for application&amp;nbsp;</td></tr><tr><td>Polyethylene bags</td><td>Johnson &amp;amp; Johnson</td><td>N/A</td><td>Other brands may be used for soil storage</td></tr><tr><td>Reciprocating saw</td><td>Black &amp;amp; Decker&amp;nbsp;</td><td>N/A</td><td>Any reciprocating saw can be used with a metal cutting attachment</td></tr></tbody></table>

integrated field lysimetry and porewater sampling for evaluation of chemical mobility in soils and established vegetation

潜在的有毒化学品的常规应用到土地，以满足废物管理和粮食生产日益增长的需求，但这些化学品的命运往往不能很好地理解。在这里，我们展示了一个集成领域lysimetry和孔隙水采样的方法，用于评估施用到土壤和植被建立化学品的流动性。渗仪，金属或塑料制成的空心柱，打入bareground或植物的土壤。孔隙水取样器，它是市售的，并使用真空收集渗透的土壤水分，被安装在所述测渗仪内的预定深度。在下列化学应用到试验田预先安排的时间，​​孔隙水被收集，并且渗仪，包含土壤和植被，被挖掘出来。通过分析蒸渗仪土壤，植被和孔隙水，向下的浸出率，土壤保持能力，以及植物吸收的利息化学化学浓度可以量化。由于现场lysimetry和孔隙水采样是根据自然环境条件和土壤干扰最小进行的，得出结果项目实际情况的方案，并提供有价值的信息，为化学品管理。由于化学品越来越多地应用到全世界的土地，所描述的技术可用于确定应用的化学物质是否对不良反应对人体健康或环境。

这种方法允许对化学品的命运数据的积累应用到bareground和植被土壤系统5,10。这种方法被用来评估砷（以As计）向下淋溶，吸收和易位转化为狗牙根植物（ 狗牙根 ）以下应用的有机砷除草剂味精甲基砷酸（MSMA）系统9。自1960年以来，MSMA已用于非农田，草坪草和棉花产量，但是可能有应用由于可以通过土壤向下渗漏，污染地下水11,12日益受到关注。美国环境保护署（E...

Watch this Scientific Journal Video about Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation at JoVE.com

Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation

现场lysimetry和孔隙水采样允许研究人员评估施用到土壤和植被建立化学品的命运。本协议的目的是演示如何安装所需的仪表和过程集成领域lysimetry和孔隙水采样实验收集样本，进行化学分析。

综合外地Lysimetry和孔隙水采样的化学活动性土壤的评价和植被成立

Potentially toxic chemicals are routinely applied to land to meet growing demands on waste management and food production, but the fate of these chemicals ...

integrated-field-lysimetry-porewater-sampling-for-evaluation-chemical

Research

JoVE Journal

Environment

14.2K 次观看.  North Carolina State University. 现场lysimetry和孔隙水采样允许研究人员评估施用到土壤和植被建立化学品的命运。本协议的目的是演示如何安装所需的仪表和过程集成领域lysimetry和孔隙水采样实验收集样本，进行化学分析。 

视频: Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation

A Protocol for Collecting and Constructing Soil Core Lysimeters

Leaching of nutrients from land applied fertilizers and manure used in agriculture can lead to accelerated eutrophication of surface water. Because the landscape has complex and varied soil morphology, an accompanying disparity in flow paths for leachate through the soil macropore and matrix structure is present. The rate of flow through these paths is further affected by antecedent soil moisture. Lysimeters are used to quantify flow rate, volume of water and concentration of nutrients leaching downward through soils. While many lysimeter designs exist, accurately determining the volume of water and mass balance of nutrients is best accomplished with bounded lysimeters that leave the natural soil structure intact. 
Here we present a detailed method for the extraction and construction of soil core lysimeters equipped with soil moisture sensors at 5 cm and 25 cm depths. Lysimeters from four different Coastal Plain soils (Bojac, Evesboro, Quindocqua and Sassafras) were collected on the Delmarva Peninsula and moved to an indoor climate controlled facility. Soils were irrigated once weekly with the equivalent of 2 cm of rainfall to draw down soil nitrate-N concentrations. At the end of the draw down period, poultry litter was applied (162 kg TN ha-1) and leaching was resumed for an additional five weeks. Total recovery of applied irrigation water varied from 71% to 85%. Nitrate-N concentration varied over the course of the study from an average of 27.1 mg L-1 before litter application to 40.3 mg L-1 following litter application. While greatest flux of nutrients was measured in soils dominated by coarse sand (Sassafras) the greatest immediate flux occurred from the finest textured soil with pronounced macropore development (Quindocqua).

A detailed method for extraction and assembly of intact soil core lysimeters and their use for study of leachate and associated loss of nutrients from surface applied poultry litter is demonstrated.

一种用于收集和构建土芯协议测渗计

Leaching of nutrients from land applied fertilizers and manure used in agriculture can lead to accelerated eutrophication of surface water. Because the ...

科研

环境科学

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations

Studying co-evolution of hydrological and biogeochemical processes in the subsurface of natural landscapes can enhance the understanding of coupled Earth-system processes. Such knowledge is imperative in improving predictions of hydro-biogeochemical cycles, especially under climate change scenarios. We present an experimental method, designed to capture sub-surface heterogeneity of an initially homogeneous soil system. This method is based on destructive sampling of a soil lysimeter designed to simulate a small-scale hillslope. A weighing lysimeter of one cubic meter capacity was divided into sections (voxels) and was excavated layer-by-layer, with sub samples being collected from each voxel. The excavation procedure was aimed at detecting the incipient heterogeneity of the system by focusing on the spatial assessment of hydrological, geochemical, and microbiological properties of the soil. Representative results of a few physicochemical variables tested show the development of heterogeneity. Additional work to test interactions between hydrological, geochemical, and microbiological signatures is planned to interpret the observed patterns. Our study also demonstrates the possibility of carrying out similar excavations in order to observe and quantify different aspects of soil-development under varying environmental conditions and scale.

This study presents an excavation method for investigating subsurface hydrological, geochemical, and microbiological heterogeneity of a soil lysimeter. The lysimeter simulates an artificial hillslope which was initially under homogeneous condition and had been subjected to approximately 5,000 mm of water over eight cycles of irrigation in an 18-month period.

土壤蒸渗仪开挖加之水文，地球化学和微生物调查

Studying co-evolution of hydrological and biogeochemical processes in the subsurface of natural landscapes can enhance the understanding of coupled ...

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Microbial communities are important drivers and regulators of ecosystem processes. To understand how management of ecosystems may affect microbial communities, a relatively precise but effort-intensive technique to assay microbial community composition is phospholipid fatty acid (PLFA) analysis. PLFA was developed to analyze phospholipid biomarkers, which can be used as indicators of microbial biomass and the composition of broad functional groups of fungi and bacteria. It has commonly been used to compare soils under alternative plant communities, ecology, and management regimes. The PLFA method has been shown to be sensitive to detecting shifts in microbial community composition.
An alternative method, fatty acid methyl ester extraction and analysis (MIDI-FA) was developed for rapid extraction of total lipids, without separation of the phospholipid fraction, from pure cultures as a microbial identification technique. This method is rapid but is less suited for soil samples because it lacks an initial step separating soil particles and begins instead with a saponification reaction that likely produces artifacts from the background organic matter in the soil. 
This article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples. The method combines the accuracy achieved through PLFA profiling by extracting and concentrating soil lipids as a first step, and a reduction in effort by saponifying the organic material extracted and processing with the MIDI-FA method as a second step.

The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.

用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

Microbial communities are important drivers and regulators of ecosystem processes. To understand how management of ecosystems may affect microbial ...

Measuring and Mapping Patterns of Soil Erosion and Deposition Related to Soil Carbonate Concentrations Under Agricultural Management

Spatial patterns of soil erosion and deposition can be inferred from differences in ground elevation mapped at appropriate time increments. Such changes in elevation are related to changes in near-surface soil carbonate (CaCO3) profiles. The objective is to describe a simple conceptual model and detailed protocol for repeatable field and laboratory measurements of these quantities. Here, accurate elevation is measured using a ground-based differential global positioning system (GPS); other data acquisition methods could be applied to the same basic method. Soil samples are collected from prescribed depth intervals and analyzed in the lab using an efficient and precise modified pressure-calcimeter method for quantitative analysis of inorganic carbon concentration. Standard statistical methods are applied to point data, and representative results show significant correlations between changes in soil surface layer CaCO3 and changes in elevation consistent with the conceptual model; CaCO3 generally decreased in depositional areas and increased in erosional areas. Maps are derived from point measurements of elevation and soil CaCO3 to aid analyses. A map of erosional and depositional patterns at the study site, a rain-fed winter wheat field cropped in alternating wheat-fallow strips, shows the interacting effects of water and wind erosion affected by management and topography. Alternative sampling methods and depth intervals are discussed and recommended for future work relating soil erosion and deposition to soil CaCO3.

Spatial patterns of soil erosion and deposition can be inferred from differences in ground elevation mapped at appropriate time increments. Such changes in elevation are related to changes in near-surface soil carbonates. Repeatable methods for field and laboratory measurements of these quantities and data analysis methods are described here.

农业经营条件下土壤侵蚀和沉积与土壤碳酸盐浓度关系的测量与制图

Spatial patterns of soil erosion and deposition can be inferred from differences in ground elevation mapped at appropriate time increments. Such changes ...

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Natural organic matter (NOM) is composed of a highly complex mixture of thousands of organic compounds which, historically, proved difficult to characterize. However, to understand the thermodynamic and kinetic controls on greenhouse gas (carbon dioxide [CO2] and methane [CH4]) production resulting from the decomposition of NOM, a molecular-level characterization coupled with microbial proteome analyses is necessary. Further, climate and environmental changes are expected to perturb natural ecosystems, potentially upsetting complex interactions that influence both the supply of organic matter substrates and the microorganisms performing the transformations. A detailed molecular characterization of the organic matter, microbial proteomics, and the pathways and transformations by which organic matter is decomposed will be necessary to predict the direction and magnitude of the effects of environmental changes. This article describes a methodological throughput for comprehensive metabolite characterization in a single sample by direct injection Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS), gas chromatography mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy, liquid chromatography mass spectrometry (LC-MS), and proteomics analysis. This approach results in a fully-paired dataset which improves statistical confidence for inferring pathways of organic matter decomposition, the resulting CO2 and CH4 production rates, and their responses to environmental perturbation. Herein we present results of applying this method to NOM samples collected from peatlands; however, the protocol is applicable to any NOM sample (e.g., peat, forested soils, marine sediments, etc.).

This protocol describes a single throughput for complementary analytical and omics techniques culminating in a fully-paired characterization of natural organic matter and microbial proteomics in different ecosystems. This approach permits robust comparisons for identifying metabolic pathways and transformations important for describing greenhouse gas production and predicting responses to environmental change.

用于表征复杂天然有机物混合物的单吞吐量互补高分辨率分析技术

Natural organic matter (NOM) is composed of a highly complex mixture of thousands of organic compounds which, historically, proved difficult to ...

Measuring Phosphorus Release in Laboratory Microcosms for Water Quality Assessment

Phosphorus (P) is a critical limiting nutrient in agroecosystems requiring careful management to reduce transport risk to aquatic environments. Routine laboratory measures of P bioavailability are based on chemical extractions performed on dried samples under oxidizing conditions. While useful, these tests are limited with respect to characterizing P release under prolonged water saturation. Labile orthophosphate bound to oxidized iron and other metals can rapidly desorb to solution in reducing environments, increasing P mobilization risk to surface runoff and groundwater. To better quantify P desorption potential and mobility during extended saturation, a laboratory microcosm method was developed based on repeated sampling of porewater and overlying floodwater over time. The method is useful for quantifying P release potential from soils and sediments varying in physicochemical properties and can improve site-specific P mitigation efforts by better characterizing P release risk in hydrologically active areas. Advantages of the method include its ability to simulate in situ dynamics, simplicity, low cost, and flexibility.

Accurate quantification of phosphorus (P) desorption potential in saturated soils and sediments is important for P modeling and transport mitigation efforts. To better account for in situ soil-water redox dynamics and P mobilization under prolonged saturation, a simple approach was developed based on repeated sampling of laboratory microcosms.

测量实验室微量微生物中的磷释放,用于水质评估

Phosphorus (P) is a critical limiting nutrient in agroecosystems requiring careful management to reduce transport risk to aquatic environments. Routine ...

Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

We describe a method for two-dimensional (2D) visualization and quantification of the distribution of labile (i.e., reversibly adsorbed) inorganic nutrient (e.g., P, Fe, Mn) and contaminant (e.g., As, Cd, Pb) solute species in the soil adjacent to plant roots (the &#8216;rhizosphere&#8217;) at sub-millimeter (~100 &#181;m) spatial resolution. The method combines sink-based solute sampling by the diffusive gradients in thin films (DGT) technique with spatially resolved chemical analysis by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The DGT technique is based on thin hydrogels with homogeneously distributed analyte-selective binding phases. The variety of available binding phases allows for the preparation of different DGT gel types following simple gel fabrication procedures. For DGT gel deployment in the rhizosphere, plants are grown in flat, transparent growth containers (rhizotrons), which enable minimal invasive access to a soil-grown root system. After a pre-growth period, DGT gels are applied to selected regions of interest for in situ solute sampling in the rhizosphere. Afterwards, DGT gels are retrieved and prepared for subsequent chemical analysis of the bound solutes using LA-ICP-MS line-scan imaging. Application of internal normalization using 13C and external calibration using matrix-matched gel standards further allows for the quantification of the 2D solute fluxes. This method is unique in its capability to generate quantitative, sub-mm scale 2D images of multi-element solute fluxes in soil-plant environments, exceeding the achievable spatial resolution of other methods for measuring solute gradients in the rhizosphere substantially. We present the application and evaluation of the method for imaging multiple cationic and anionic solute species in the rhizosphere of terrestrial plants and highlight the possibility of combining this method with complementary solute imaging techniques.

This protocol presents a workflow for sub-mm 2D visualization of multiple labile inorganic nutrient and contaminant solute species using diffusive gradients in thin films (DGT) combined with mass spectrometry imaging. Solute sampling and high-resolution chemical analysis are described in detail for quantitative mapping of solutes in the rhizosphere of terrestrial plants.

土壤中实验室、无机植物养分和污染物的二维可视化和量化

We describe a method for two-dimensional (2D) visualization and quantification of the distribution of labile (i.e., reversibly adsorbed) inorganic ...

In Situ Soil Moisture Sensors in Undisturbed Soils

Soil moisture directly affects operational hydrology, food security, ecosystem services, and the climate system. However, the adoption of soil moisture data has been slow due to inconsistent data collection, poor standardization, and typically short record duration. Soil moisture, or quantitatively volumetric soil water content (SWC), is measured using buried, in situ sensors that infer SWC from an electromagnetic response. This signal can vary considerably with local site conditions such as clay content and mineralogy, soil salinity or bulk electrical conductivity, and soil temperature; each of these can have varying impacts depending on the sensor technology.
Furthermore, poor soil contact and sensor degradation can affect the quality of these readings over time. Unlike more traditional environmental sensors, there are no accepted standards, maintenance practices, or quality controls for SWC data. As such, SWC is a challenging measurement for many environmental monitoring networks to implement. Here, we attempt to establish a community-based standard of practice for in situ SWC sensors so that future research and applications have consistent guidance on site selection, sensor installation, data interpretation, and long-term maintenance of monitoring stations.
The videography focuses on a multi-agency consensus of best-practices and recommendations for the installation of in situ SWC sensors. This paper presents an overview of this protocol along with the various steps essential for high-quality and long-term SWC data collection. This protocol will be of use to scientists and engineers hoping to deploy a single station or an entire network.

In Situ Soil Moisture Sensors in Undisturbed Soils

The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors.

原位 未受干扰土壤中的土壤湿度传感器

Soil moisture directly affects operational hydrology, food security, ecosystem services, and the climate system. However, the adoption of soil moisture ...

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Biogeochemical processes shift rapidly in both spatial (millimeter scale) and temporal (hour scale to day scale) dimensions at the oxic-anoxic interface in response to disturbances. Deciphering the rapid biogeochemical changes requires in situ, minimally invasive tools with high spatial and temporal sampling resolution. However, the available passive sampling devices are not very useful in many cases either due to their disposable nature or the complexity and extensive workload for sample preparation.
To address this problem, a microdialysis profiler with 33 individual polyethersulfone nanomembrane tubes (semipermeable, &#60;20 nm pore size) integrated into the one-dimensional skeleton (60 mm) was established to iteratively sample the dissolved compounds in porewater across the soil-water interface at a high resolution of 1.8 mm (outer diameter plus one spacing, i.e., 0.1 mm between probes). The sampling mechanism is based on the principle of concentration gradient diffusion. The automatic loading of degassed water allows minimal disturbance to the chemical species across the oxic-anoxic interface.
This paper describes the procedures of device setup and continuous porewater sampling across the soil-water interface on a daily basis. Concentration-depth profiles were selectively measured before (on Day 6) and after (on Day 7) disturbances induced by irrigation. The results showed that concentration-depth profiles were undergoing rapid changes, especially for redox-sensitive elements (i.e., iron and arsenic). These protocols can help investigate the biogeochemical responses across the soil-water interface under various disturbances caused by physical, chemical, and biological factors. The paper thoroughly discusses the advantages and disadvantages of this method for potential use in the environmental sciences.

A microdialysis profiler is described to sample dissolved porewater solutes across an oxic-anoxic soil-water interface in situ with minimal disturbance. This device is designed to capture rapid changes in concentration-depth profiles induced by disturbances at the soil-water interface and beyond.

使用微透析剖面仪在氧化-缺氧土壤-水界面上进行溶解溶质采样

Biogeochemical processes shift rapidly in both spatial (millimeter scale) and temporal (hour scale to day scale) dimensions at the oxic-anoxic interface ...

Determination of Moisture Content in Soil

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona 
Demonstrating Author: Bradley Schmitz
Soils normally contain a finite amount of water, which can be expressed as the &#8220;soil moisture content.&#8221; This moisture exists within the pore spaces in between soil aggregates (inter-aggregate pore space) and within soil aggregates (intra-aggregate pore space) (Figure 1). Normally this pore space is occupied by air and/or water. If all the pores are occupied by air, the soil is completely dry. If all the pores are filled with water, the soil is said to be saturated.
<img alt="Figure 1" src="/files/ftp_upload/10011/10011fig1.jpg" /> 
Figure 1. Pore space in soil.

在土壤中的水分含量的测定

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona
Demonstrating Author: Bradley Schmitz
Soils normally contain a ...

综合外地Lysimetry和孔隙水采样的化学活动性土壤的评价和植被成立

摘要

探索更多视频

此视频中的章节

一种用于收集和构建土芯协议测渗计

土壤蒸渗仪开挖加之水文，地球化学和微生物调查

用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

农业经营条件下土壤侵蚀和沉积与土壤碳酸盐浓度关系的测量与制图

用于表征复杂天然有机物混合物的单吞吐量互补高分辨率分析技术

测量实验室微量微生物中的磷释放,用于水质评估

土壤中实验室、无机植物养分和污染物的二维可视化和量化

原位未受干扰土壤中的土壤湿度传感器

使用微透析剖面仪在氧化-缺氧土壤-水界面上进行溶解溶质采样

在土壤中的水分含量的测定

综合外地Lysimetry和孔隙水采样的化学活动性土壤的评价和植被成立

摘要

探索更多视频

此视频中的章节

一种用于收集和构建土芯协议测渗计

土壤蒸渗仪开挖加之水文，地球化学和微生物调查

用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

农业经营条件下土壤侵蚀和沉积与土壤碳酸盐浓度关系的测量与制图

用于表征复杂天然有机物混合物的单吞吐量互补高分辨率分析技术

测量实验室微量微生物中的磷释放,用于水质评估

土壤中实验室、无机植物养分和污染物的二维可视化和量化

原位 未受干扰土壤中的土壤湿度传感器

使用微透析剖面仪在氧化-缺氧土壤-水界面上进行溶解溶质采样

在土壤中的水分含量的测定

原位未受干扰土壤中的土壤湿度传感器