Synthesis of a Halogenated Indoxylic Acid Allyl Ester
4:32
Synthesis of (5-bromo-4-chloro-indol-3-yl)-β-ᴅ-galactopyranoside: X-Gal
7:01
Illustrating Model Experiment: Activity Proof
7:44
Results: Cleavage of Synthetic X-Gal by β-galactosidase
8:29
Conclusion
Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good yields.
