March 23rd, 2015
•This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.
Related Videos
Proper Care and Cleaning of the Microscope
Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Optical Mapping of Langendorff-perfused Rat Hearts
Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart