Synthesis of Manganese Prussian Blue (MnPB) Nanoparticles
4:09
Biofunctionalization and Characterization of MnPB Nanoparticles
5:29
Fluorescent Labeling of Cells with Nanoparticles
6:59
Generating MRI Contrast on Cells with Nanoparticles
9:01
Results: Imaging of Cells Labeled with Prussian Blue Nanoparticles
10:34
Conclusion
This protocol describes the synthesis of biofunctionalized Prussian blue nanoparticles and their use as multimodal, molecular imaging agents. The nanoparticles have a core-shell design where gadolinium or manganese ions within the nanoparticle core generate MRI contrast. The biofunctional shell contains fluorophores for fluorescence imaging and targeting ligands for molecular targeting.
