JOVE JOURNAL

Bioengineering

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Hybridizing Bacterial Cells with an Oligonucleotide Probe Labeled with a Fluorophore Using Fluorescence In Situ Hybridization (FISH)

Journal (Bioengineering)

Hybridizing Bacterial Cells with an Oligonucleotide Probe Labeled with a Fluorophore Using Fluorescence In Situ Hybridization (FISH)

Zhongyue Guo*¹, Yeran Bai*²^,³, Fátima C. Pereira⁴, Ji-Xin Cheng¹^,⁵

¹Department of Biomedical Engineering, Boston University, ²Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, ³Photothermal Spectroscopy Corp., ⁴School of Biological Sciences, University of Southampton, ⁵Department of Electrical & Computer Engineering, Photonics Center, Boston University

Sample Preparation for OPTIR-FISH to Probe Glucose Metabolism in Bacterial Communities

Journal (Bioengineering)

Sample Preparation for OPTIR-FISH to Probe Glucose Metabolism in Bacterial Communities

Zhongyue Guo*¹, Yeran Bai*²^,³, Fátima C. Pereira⁴, Ji-Xin Cheng¹^,⁵

¹Department of Biomedical Engineering, Boston University, ²Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, ³Photothermal Spectroscopy Corp., ⁴School of Biological Sciences, University of Southampton, ⁵Department of Electrical & Computer Engineering, Photonics Center, Boston University

Consistent Generation of Human Vascular Organoids Using Microwell Technology

Journal (Bioengineering)

Consistent Generation of Human Vascular Organoids Using Microwell Technology

Cong Xu*¹, Siyu He*¹, Yuefei Zhu¹, Gazelle Crasto², Caroline Chen¹, Morgan L. Clay³, Yeh-Hsing Lao³, Kam W. Leong¹

¹Department of Biomedical Engineering, Columbia University, ²College of Dental Medicine, Division of Periodontics, Columbia University, ³Department of Pharmaceutical Sciences, University at Buffalo, The State University of New York

Designing sgRNA and Construction of Vector for Genome Editing to Breed Rice with Resistant Starch

Journal (Bioengineering)

Designing sgRNA and Construction of Vector for Genome Editing to Breed Rice with Resistant Starch

Chunxue Yan¹^,², Huicong Meng¹^,², Yanxin Pei¹^,², Wei Sun², Jinshan Zhang¹^,²

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences, Shandong Normal University

Resistant Starch Content Measurement in Rice Varieties Developed Through Genome Editing

Journal (Bioengineering)

Resistant Starch Content Measurement in Rice Varieties Developed Through Genome Editing

Chunxue Yan¹^,², Huicong Meng¹^,², Yanxin Pei¹^,², Wei Sun², Jinshan Zhang¹^,²

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences, Shandong Normal University

Genotyping Transgenic Plants and Harvesting Seeds for Selecting Rice Varieties Enriched with Resistant Starch

Journal (Bioengineering)

Genotyping Transgenic Plants and Harvesting Seeds for Selecting Rice Varieties Enriched with Resistant Starch

Chunxue Yan¹^,², Huicong Meng¹^,², Yanxin Pei¹^,², Wei Sun², Jinshan Zhang¹^,²

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences, Shandong Normal University

Analysis of Osteochondral Explants Using Micro-CT and Deep Learning Segmentation Techniques

Journal (Bioengineering)

Analysis of Osteochondral Explants Using Micro-CT and Deep Learning Segmentation Techniques

Constance Lesage¹, Pierre Guihard¹, Claire De Fourmestraux¹, Olivier Gauthier¹, Pierre Weiss¹, Jérome Guicheux¹, Aleksandra Mieczkowska¹, Joelle Véziers¹, Baptiste Charbonnier¹, Marie-Astrid Boutet¹, Guillaume Mabilleau¹^,²

¹Nantes Université, Univ Angers, Oniris, CHU Nantes, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, ²CHU Angers, Departement pathologie cellulaire et tissulaire

Osteochondral Bone Explant Preparation for Comprehensive Assessment of the Quality of Regenerated Bone Tissues

Journal (Bioengineering)

Osteochondral Bone Explant Preparation for Comprehensive Assessment of the Quality of Regenerated Bone Tissues

Constance Lesage¹, Pierre Guihard¹, Claire De Fourmestraux¹, Olivier Gauthier¹, Pierre Weiss¹, Jérome Guicheux¹, Aleksandra Mieczkowska¹, Joelle Véziers¹, Baptiste Charbonnier¹, Marie-Astrid Boutet¹, Guillaume Mabilleau¹^,²

¹Nantes Université, Univ Angers, Oniris, CHU Nantes, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, ²CHU Angers, Departement pathologie cellulaire et tissulaire

Preparation of Lyophilized Reverse Transcriptase-Recombinase Polymerase Amplification (RT-RPA) Reactions

Journal (Bioengineering)

Preparation of Lyophilized Reverse Transcriptase-Recombinase Polymerase Amplification (RT-RPA) Reactions

Rodjarin Kongkaew¹, Chayasith Uttamapinant¹, Maturada Patchsung¹

¹School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC)

RT-RPA Nucleic Acid Amplification and CRISPR-Cas13 Nucleic Acid Detection

Journal (Bioengineering)

RT-RPA Nucleic Acid Amplification and CRISPR-Cas13 Nucleic Acid Detection

Rodjarin Kongkaew¹, Chayasith Uttamapinant¹, Maturada Patchsung¹

¹School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC)

Preparation of Lyophilized CRISPR-Cas13a Premixed Detection Reagents for Nucleic Acid Biomarkers Detection

Journal (Bioengineering)

Preparation of Lyophilized CRISPR-Cas13a Premixed Detection Reagents for Nucleic Acid Biomarkers Detection

Rodjarin Kongkaew¹, Chayasith Uttamapinant¹, Maturada Patchsung¹

¹School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC)

Preparation of Viral Sample and Master Mix for Digital Droplet PCR to Accurately Measure the Viral Genome Copy Number

Journal (Bioengineering)

Preparation of Viral Sample and Master Mix for Digital Droplet PCR to Accurately Measure the Viral Genome Copy Number

Nathalie Van den Berghe¹, Elien Costermans¹, Samir Nuseibeh¹, Tine Brouns¹, Inge Van Hove¹, Benjamien Moeyaert¹, Els Henckaerts¹

¹Trellis Research Group, Department of Cellular and Molecular Medicine, Department of Microbiology, Immunology and Transplantation, KU Leuven

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