September 27th, 2015
•Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.
Related Videos
Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay
Plastic Embedding and Sectioning of Xenopus laevis Embryos
Obtaining Eggs from Xenopus laevis Females
Preparation and Fractionation of Xenopus laevis Egg Extracts
Microinjection of Xenopus Laevis Oocytes
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
Facial Transplants in Xenopus laevis Embryos