JoVE Logo
教师资源中心

登录

Preparation and Fractionation of Xenopus laevis Egg Extracts

DOI :

10.3791/891-v

August 27th, 2008

August 27th, 2008

12,095 Views

1Department of Cell Biology, Emory University

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Tags

Preparation

-- Views

Related Videos

article

Plastic Embedding and Sectioning of Xenopus laevis Embryos

article

Obtaining Eggs from Xenopus laevis Females

article

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

article

Microinjection of Xenopus Laevis Oocytes

article

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

article

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

article

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

article

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

article

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

article

Facial Transplants in Xenopus laevis Embryos

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。