Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

DOI :

10.3791/53106-v

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9:11 min

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February 23rd, 2016

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20,514 Views

Christina Wayman^1,2*, Denise A. Duricki^1,2*, Lisa A. Roy³, Barbara Haenzi¹, Shi-Yen Tsai⁴, Gwendolyn Kartje^4,5,6, John S. Beech⁷, Diana Cash², Lawrence Moon¹

¹Wolfson Centre for Age-Related Diseases, King's College London, University of London, ²Department of Neuroimaging, James Black Centre, Institute of Psychiatry, King's College London, University of London, ³Institute of Neuroscience and Psychology, Wellcome Surgical Institute, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, ⁴Research Service, Edward Hines Jr. VA Hospital, ⁵Neurology Service, Edward Hines Jr. VA Hospital, ⁶Department of Molecular Pharmacology and Therapeutics, Neuroscience Research Institute, Loyola University Chicago, ⁷Department of Oncology, The Gray Institute for Radiation, Oncology and Biology, University of Oxford

Chapters

Here we present a protocol to produce permanent distal middle cerebral artery occlusion in elderly female rats with simultaneous occlusion of the carotid arteries to generate large cortical infarcts and sustained deficits. We show confirmation of the lesion size using structural MRI at 24 hr and 8 weeks after stroke.

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