A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
March 5th, 2016
•Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
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Low-Density Primary Hippocampal Neuron Culture
