Synthesis via Citrate Reduction of Gold Nanoparticles (AuNP) and Characterization
2:35
DNA-aptamer, Buffer, Solution, and Assay Preparation
3:35
Salt Titration and Assay Setup
5:21
Freezing Aptamer–AuNP Assay for Long-term Storage
6:38
Results: Assay Response with Changing DNA Coverage Density and Performance of the Assay for Long-term Storage
7:37
Conclusion
副本
The overall goal is to provide a general approach for the development of an aptamer-gold nanoparticle based colorimetric assay for the detection of target molecules, and to provide approaches to improve long-term storage and reduce false positive
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The design and development of an aptamer–gold nanoparticle colorimetric assay for the detection of small molecules for in-the-field applications was examined. Additionally, a smart-device colorimetric application (app) was validated and long-term storage of the assay was established for use in the field.