The overall goal of this protocol is to demonstrate the fabrication and operation of a microfluidic chip, which allows for high-efficiency single-cell isolation and culture. This method provides a useful tool for any area that would benefit from s
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Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.