Preparation of Cationic Liposome-siRNA-Peptide Complexes (LSPCs)
3:25
Preparation of Peptide Addressed Liposome Encapsulated Therapeutic siRNA (PALETS)
6:03
Tail-vein Injection of LSPCs or PALETS
7:09
Analysis of Protein Expression by Flow Cytometry
9:14
Results: Representative Flow Cytometric Analysis of Brian Cell Suspensions 24 hr After Treatment with LSPCs
9:56
Conclusion
副本
The overall goal of this procedure is to design a delivery system that can deliver small molecule drugs like siRNA to the brain. This method can answer key questions in the neurodegeneration field such as Is siRNA a viable therapeutic option for p
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The goal of this protocol is to use cationic/anionic liposomes with a neuro-targeting peptide as a CNS delivery system to enable siRNA to cross the BBB. The optimization of a delivery system for treatments, like siRNA, would allow for more treatment options for prion and other neurodegenerative diseases.
