Name: murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with ovarian carcinoma cells
Uploaded: 2016-12-09T12:30:00.000+00:00
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Description: Watch this Scientific Journal Video about Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells at JoVE.com

作者感谢妇产科系的成员和癌症生物学为他们有益的讨论和技术援助。 （;到H梶山15K15604）这项研究是由日本学术振兴会（JSPS）赠款在资助科学研究的支持。

该治疗方案遵循由名古屋大学通过动物实验的指导方针。 1.悬浮细胞的制备<ol><li>人卵巢透明细胞癌的细胞系。 <ol><li>维持ES-2细胞12中，用10％胎牛血清（FBS）和青霉素/链霉素的RPMI-1640培养基（青霉素:100单位/ ml，链霉素:0.1毫克/毫升）。在一个5％CO 2的加湿培养箱中培养细胞在37℃。 </li><li>收集在对数生长期的细胞在0.05％胰蛋白酶/ 0.02％EDTA溶液（胰蛋白酶/ EDTA）。 <ol><li>首先，从细胞培养容器中删除旧的介质，然后用PBS洗涤细胞一次（PBS不含Ca 2+ / Mg 2+离子 ，3 -第4毫升，每为25cm 2）。 </li><li>接下来，添加每25厘米胰蛋白酶/ EDTA的2 1毫升旋转细胞培养容器以覆盖与胰蛋白酶/ EDTA的细胞单层。拆除和丢弃胰蛋白酶/ EDTA后，返回小区培养容器的孵化器3 - 5分钟或直至细胞被分离。 </li><li>在显微镜下检查细胞以确保将细胞从细胞培养容器中，浮动的表面脱离。 </li><li>添加含有生长培养基以灭活胰蛋白酶（5毫升，每为25cm 2），并重新悬浮细胞新鲜血清。传送再悬浮细胞至15ml锥形管中。 </li><li>离心在300×g离心5分钟。在4℃或室温下进行离心任。小心取出上清，重新暂停与生长培养基细胞（每为25cm 2 5ml）中。 </li></ol></li><li>计数细胞（ 例如 ，通过台盼蓝排除），然后在生长培养基中以2×10 8个细胞/ ml的密度重新悬浮。把悬浮细胞冰上。 </li></ol></li><li>解冻冷冻的细胞外基质（ECM）为基础的水凝胶的等分试样在冰浴中。 </li><li>添加细胞悬浮液，以基于ECM的水凝胶的等分试样在1:1的比例（终小区concentration:1×10 5细胞/基于中等的ECM的水凝胶的微升）并充分混合。放置制备的细胞悬浮液在冰浴中直至使用。 </li></ol> 2.原位接种悬浮细胞注意:手术不需要专用的套件。手术可以在实验室台式在是单独的，并具有最小的活动房的区域来执行。层流罩也可被使用。无菌手术器械必须使用剪刀不应该被用来使皮肤切口。 <ol><li>使用8周龄的雌性裸鼠（BALB / c小鼠NU / NU）此原位接种模型。 </li><li>首先，麻醉用吸入异氟烷鼠标（2 - 4％）。通过验证后，缺乏坚定的脚趾捏撤回检查麻醉深度。鼠标麻醉后，将一个平淡无菌眼药膏的眼睛，以防止干燥是必要的。 </li><li>放置鼠标麻醉上OPERATING阶段腹侧下来。消毒手术区数次酒精和一个基于洗必泰碘基或擦洗。 </li><li>使靠近垂直右肋弓与股骨之间的脊椎1〜2厘米的切口。使用切口周围部位的无菌手术盖布。用夹子固定修复皮肤切表面，以保持开放。 注意:拉钩可被用来代替夹具。 </li><li>接着，使一第二切口（大约1厘米）在壁层腹膜第一切口下，打开腹腔。夹紧阴刻腹部表面。夹与夹子围绕患侧卵巢和输卵管从腹腔取出卵巢的脂肪组织。小心地放在纱布卵巢和输卵管。然后，将鼠标腹侧下解剖显微镜下。 </li><li>将细胞悬液中胰岛素注射器（1/2毫升，29克）刚刚注射前。小心注入所制备的细胞悬浮液（1- 2微升）进卵巢。几秒，保留卵巢内的针，以确保基于ECM的水凝胶的凝胶化。凝胶化后，注入肿瘤细胞保持卵巢内。 </li><li>仔细返回卵巢到体内。缝合无菌医疗丝绸第二切口，然后用密封夹伤皮肤。 </li><li>手术后，将小鼠施用初始24小时的时间内的镇痛（ 例如 ，美洛昔康，肌内（IM）或皮下（sc），0.2毫克/千克）。 </li></ol> 3.手术后的治疗<ol><li>将在干净的纸单独的笼子每只小鼠用热敷，以保持温暖鼠标，直到它已经恢复了足够的意识，以保持胸骨斜卧。然后，返回的动物笼子里饲养和监控。每天监控小鼠3 - 7日之后进行手术治疗。 </li><li>术后小鼠监控<ol><li>在疼痛的迹象的情况下，管理analgeSICS每天（ 如美洛昔康，IM或SC，0.2毫克/千克）。 </li><li>如果小鼠有感染（ 如发红，肿胀，放电）的迹象，每天给药注射抗感染剂（ 如青霉素，即时通讯，10 IU /公斤）。 </li></ol></li><li>手术后的治疗后14天 - 取下皮肤缝合材料10。 </li></ol> 4.肿瘤和转移的分析注意:肿瘤会在注射子房2形成 - 3周接种后。 <ol><li>牺牲由二氧化碳的小鼠，然后收集样品（ 例如 ，肿瘤，转移瘤，器官）13-15作进一步的分析14,16-20。 注:如果在体内成像系统是可用的，轴承产生生物发光（ 如萤光素酶）或荧光蛋白（ 例如 ，GFP）的酶的细胞可以在这个协议中使用。这个修改后的协议将提供有用的信息癌细胞从原发肿瘤扩散的动力学。 </li></ol>

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作者什么都没有透露。

动物模型是分析肿瘤的发展，进展，转移，和药物疗效的机理对癌细胞是必不可少的。荷人卵巢癌细胞也已用作动物模型。这里，我们描述在原位的网站卵巢肿瘤细胞的给药的微创过程。通过从背侧面腹膜后方法使用这一步骤，接种到卵巢优于其它常用的技术提供显著优点。首先，原位接种可以提供反映在人类13,21肿瘤环境更有效和准确的结果。其次，从背侧此腹膜后方法只需要一个小的...

上皮性卵巢癌（EOC）占癌症相关死亡率的妇科恶性肿瘤1中的最高速度。 EOC与预后较差，主要是由于其后期症状，并经常与多个浸染腹腔及远处转移有关2-4。腹膜传播是一个多步骤的过程。首先，肿瘤细胞从原发病灶脱落，并迁移进入腹腔。当肿瘤细胞附着到腹膜间皮后，便开始通过间皮5,6-侵入组织。为了更好地理解肿瘤生物学（ 如癌症进展和治疗反应），小鼠模型提供了丰富的信息。与人癌细胞的异种移植物被广泛用于其中将人癌细胞皮下接种，腹膜内，静脉内，以及原位小鼠模型。与原位克动物模型筏肿瘤可以更有效地和精确地生成反映在人类肿瘤环境中与一个异位接枝肿瘤的动物模型中比较结果。因此，对许多人类肿瘤，原位移植模型已经建立7-11。 这里，我们描述通过从背侧面，这限制了侵袭性相比腹侧接种腹膜后途径的人类EOC细胞的原位接种。这种技术可提供各种对EOC有用的信息，特别是腹膜内传播的机制。

<table><tbody><tr><td>Matrigel matrix&amp;nbsp;</td><td>BD</td><td>354234</td><td>ECM-based hydrogel</td></tr><tr><td>Insulin syringe</td><td>TERUMO</td><td>SS-05M2913</td><td>1/2 cc, 29 G x 1/2&quot;</td></tr><tr><td>Suturing needle with suture</td><td></td><td></td><td>11 mm, 3/8, Nylon6-0</td></tr><tr><td>Reflex 7 mm Wound Clip Applier</td><td>CellPoint Scientific</td><td>204-1000</td><td></td></tr><tr><td>&amp;nbsp;Reflex 7 mm wound clips</td><td>CellPoint Scientific</td><td>203-1000</td><td></td></tr></tbody></table>

murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with ovarian carcinoma cells

上皮性卵巢癌（EOC）与预后不良，因为它表明腹膜转移有关。改善预后，以控制腹膜是重要的。然而，目前还不清楚的肿瘤细胞是如何从原发病灶分离并附着到间皮。适当的动物模型的建立需要获得体内腹膜的机制的理解。在目前的研究中，我们介绍从局部注射EOC细胞进入鼠卵巢表面向转移的发展，包括腹膜及远处器官的过程。在8周龄雌性裸鼠（BALB / c小鼠NU / NU）被使用。下的视显微镜视野，EOC细胞（1×10 5细胞/中细胞外基质（ECM）的微升为基础的水凝胶/单侧卵巢/小鼠）通过从背侧面腹膜后途径注射到鼠卵巢。这种方法是一种乐为鼠标SS侵入性的程序，最大限度地减少对卵巢的伤害。在这里，我们描述了在原始和转移性肿瘤的形成的EOC的发展的方法步骤。

<html>六裸鼠接种了与人类透明细胞癌细胞系ES-2自己的卵巢，如本协议所述。如在图1A中所示，2周后，将6只小鼠的5例中具有ES-2细胞接种卵巢肿瘤，但有在对侧卵巢肿瘤无无接种（用作对照）。此外，5只小鼠的2显示腹水多腹膜浸染。细胞转移到肝脏（ 图1B）。卵巢，都与在接种部位和在控制侧上的肿瘤块，进行解剖，切片，用苏木精-曙红（HE）染...

Watch this Scientific Journal Video about Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells at JoVE.com

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

澄清卵巢癌的腹膜传播的多步骤的过程，目前的研究提出了原发肿瘤的发展和腹膜转移通过原位接种这些肿瘤细胞的鼠实验模型。

原发肿瘤发展小鼠实验模型和腹膜转移<em&gt;通过</em&gt;原位接种卵巢癌细胞

Epithelial ovarian carcinoma (EOC) is associated with a poor prognosis because it shows peritoneal dissemination. To improve the prognosis, it is ...

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Cancer Research

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

10.4K 次观看.  Bell Research Center for Reproductive Health and Cancer. 澄清卵巢癌的腹膜传播的多步骤的过程，目前的研究提出了原发肿瘤的发展和腹膜转移通过原位接种这些肿瘤细胞的鼠实验模型。 

视频: Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

Background: Ovarian cancer is generally diagnosed at an advanced stage where the case/fatality ratio is high and thus remains the most lethal of all gynecologic malignancies among US women 1,2,3. Serous tumors are the most widespread forms of ovarian cancer and 4,5 the Tg-MISIIR-TAg transgenic represents the only mouse model that spontaneously develops this type of tumors. Tg-MISIIR-TAg mice express SV40 transforming region under control of the Mullerian Inhibitory Substance type II Receptor (MISIIR) gene promoter 6. Additional transgenic lines have been identified that express the SV40 TAg transgene, but do not develop ovarian tumors. Non-tumor prone mice exhibit typical lifespan for C57Bl/6 mice and are fertile. These mice can be used as syngeneic allograft recipients for tumor cells isolated from Tg-MISIIR-TAg-DR26 mice.
Objective: Although tumor imaging is possible 7, early detection of deep tumors is challenging in small living animals. To enable preclinical studies in an immunologically intact animal model for serous ovarian cancer, we describe a syngeneic mouse model for this type of ovarian cancer that permits in vivo imaging, studies of the tumor microenvironment and tumor immune responses.
Methods: We first derived a TAg+ mouse cancer cell line (MOV1) from a spontaneous ovarian tumor harvested in a 26 week-old DR26 Tg-MISIIR-TAg female. Then, we stably transduced MOV1 cells with TurboFP635 Lentivirus mammalian vector that encodes Katushka, a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor with excitation/emission maxima at 588/635 nm 8,9,10. We orthotopically implanted MOV1Kat in the ovary 11,12,13,14 of non-tumor prone Tg-MISIIR-TAg female mice. Tumor progression was followed by in vivo optical imaging and tumor microenvironment was analyzed by immunohistochemistry.
Results: Orthotopically implanted MOV1Kat cells developed serous ovarian tumors. MOV1Kat tumors could be visualized by in vivo imaging up to three weeks after implantation (fig. 1) and were infiltrated with leukocytes, as observed in human ovarian cancers 15 (fig. 2).
Conclusions: We describe an orthotopic model of ovarian cancer suitable for in vivo imaging of early tumors due to the high pH-stability and photostability of Katushka in deep tissues. We propose the use of this novel syngeneic model of serous ovarian cancer for in vivo imaging studies and monitoring of tumor immune responses and immunotherapies.

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.

浆液性卵巢癌原位模型在免疫小鼠，为在体内肿瘤显像和肿瘤免疫反应的监测

Background: Ovarian cancer is generally diagnosed at an advanced stage where the case/fatality ratio is high and thus remains the most lethal of all ...

科研

免疫与感染

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

Ovarian cancer is characterized by early, diffuse metastasis with 70% of women having metastatic disease at the time of diagnosis. While elegant transgenic mouse models of ovarian cancer exist, these mice are expensive and take a long time to develop tumors. Intraperitoneal injection xenograft models lack human stroma and do not accurately model ovarian cancer metastasis. Even patient derived xenografts (PDX) do not fully recapitulate the human stromal microenvironment as serial PDX passages demonstrate significant loss of human stroma. The ability to easily model human ovarian cancer within a physiologically relevant stromal microenvironment is an unmet need. Here, the protocol presents an orthotopic ovarian cancer mouse model using human ovarian cancer cells combined with patient-derived carcinoma-associated mesenchymal stem cells (CA-MSCs). CA-MSCs are stromal progenitor cells, which drive the formation of the stromal microenvironment and support ovarian cancer growth and metastasis. This model develops early and diffuses metastasis mimicking clinical presentation. In this model, luciferase expressing ovarian cancer cells are mixed in a 1:1 ratio with CA-MSCs and injected into the ovarian bursa of NSG mice. Tumor growth and metastasis are followed serially over time using bioluminescence imaging. The resulting tumors grow aggressively and form abdominal metastases by 14 days post injection. Mice experienced significant decreases in body weight as a marker of systemic illness and increased disease burden. By day 30 post injection, mice met endpoint criteria of &gt;10% body weight loss and necropsy confirmed intra-abdominal metastasis in 100% of mice and 60%-80% lung and parenchymal liver metastasis. Collectively, orthotopic engraftment of ovarian cancer cells and stroma cells generates tumors that closely mimic the early and diffuse metastatic behavior of human ovarian cancer. Furthermore, this model provides a tool to study the role of ovarian cancer cell: stroma cell interactions in metastatic progression.

Orthotopic engraftment of ovarian cancer cells mixed with human stromal cells provides a mouse model that exhibits rapid, diffuse metastatic behavior that is characteristic of human ovarian cancer. This model also allows for the study of tumor cell and stromal cell interactions, as well as their role in tumor progression and metastasis.

Ovarian cancer is characterized by early, diffuse metastasis with 70% of women having metastatic disease at the time of diagnosis. While elegant ...

癌症研究

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Osteosarcoma is the most common primary bone cancer in children and adolescents, with lungs as the most common metastatic site. The five-year survival rate of osteosarcoma patients with pulmonary metastasis is less than 30%.Therefore, the utilization of mouse models mimicking the osteosarcoma development in humans is of great significance for understanding the fundamental mechanism of osteosarcoma carcinogenesis and pulmonary metastasis to develop novel therapeutics. Here, detailed procedures are reported to generate the primary osteosarcoma and pulmonary metastasis mouse models via&#160;intratibia injection of osteosarcoma cells. Combined with the bioluminescence or X-ray live imaging system, these living mouse models are utilized to monitor and quantify osteosarcoma growth and metastasis. To establish this model, a basement membrane matrix containing osteosarcoma cells was loaded in a micro-volume syringe and injected into one tibia of each athymic mouse after being anesthetized. The mice were sacrificed when the primary osteosarcoma reached the size limitation in the IACUC-approved protocol. The legs bearing osteosarcoma and the lungs with metastasis lesions were separated. These models are characterized by a short incubation period, rapid growth, severe lesions, and sensitivity in monitoring the development of primary and pulmonary metastatic lesions. Therefore, these are ideal models for exploring the functions and mechanisms of specific factors in osteosarcoma carcinogenesis and pulmonary metastasis, the tumor microenvironment, and evaluating the therapeutic efficacy in vivo.

The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions.

胫骨肉瘤细胞注射生成原位骨肉瘤和肺转移小鼠模型

Osteosarcoma is the most common primary bone cancer in children and adolescents, with lungs as the most common metastatic site. The five-year survival ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of care consisting of surgical debulking and combination chemotherapy consisting of platinum and taxane compounds, almost 90% of patients recur within a few years. In these patients the development of chemoresistant disease limits the efficacy of currently available chemotherapy agents and therefore contributes to the high mortality. To discover novel therapy options that can target recurrent disease, appropriate animal models that closely mimic the clinical profile of patients with recurrent ovarian cancer are required. The challenge in monitoring intra-peritoneal (i.p.) disease limits the use of i.p. models and thus most xenografts are established subcutaneously. We have developed a sensitive optical imaging platform that allows the detection and anatomical location of i.p. tumor mass. The platform includes the use of optical reporters that extend from the visible light range to near infrared, which in combination with 2-dimensional X-ray co-registration can provide anatomical location of molecular signals. Detection is significantly improved by the use of a rotation system that drives the animal to multiple angular positions for 360 degree imaging, allowing the identification of tumors that are not visible in single orientation. This platform provides a unique model to non-invasively monitor tumor growth and evaluate the efficacy of new therapies for the prevention or treatment of recurrent ovarian cancer.

We describe a non-invasive animal imaging platform that allows the detection, quantification, and monitoring of ovarian cancer growth and recurrence. This intra-peritoneal xenograft model mimics the clinical profile of patients with ovarian cancer.

小鼠模型的非侵入性成像检测和监测卵巢癌复发

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

医学

建立卵巢癌-网膜相互作用的 3D 离体模型

An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum

Ovarian cancer is the deadliest gynecologic malignancy. The omentum plays a key role in providing a supportive microenvironment to metastatic ovarian cancer cells as well as immune modulatory signals that allow tumor tolerance. However, we have limited models that closely mimic the interaction between ovarian cancer cells and adipose-rich tissues. To further understand the cellular and molecular mechanisms by which the omentum provides a pro-tumoral microenvironment, we developed a unique 3D ex vivo model of cancer cell-omentum interaction. Using human omentum, we are able to grow ovarian cancer cells within this adipose-rich microenvironment and monitor the factors responsible for tumor growth and immune regulation. In addition to providing a platform for the study of this adipose-rich tumor microenvironment, the model provides an excellent platform for the development and evaluation of novel therapeutic approaches to target metastatic cancer cells in this niche. The proposed model is easy to generate, inexpensive, and applicable to translational investigations.

作者聚焦:用于研究癌症-脂肪微环境相互作用的高级离体模型 

This protocol describes the establishment of a three-dimensional (3D) ex vivo model of cancer cell-omentum interaction. The model provides a platform for elucidating pro-tumor mechanisms within the adipose niche and for testing novel therapies.

基于人网膜的卵巢癌腹膜转移离 体 模型

Ovarian cancer is the deadliest gynecologic malignancy. The omentum plays a key role in providing a supportive microenvironment to metastatic ovarian ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Human cancer and response to therapy is better represented in orthotopic animal models. This paper describes the development of an orthotopic mouse model of ovarian cancer, treatment of cancer via oral delivery of drugs, and monitoring of tumor cell behavior in response to drug treatment in real time using in vivo imaging system. In this orthotopic model, ovarian tumor cells expressing luciferase are applied topically by injecting them directly into the mouse bursa where each ovary is enclosed. Upon injection of D-luciferin, a substrate of firefly luciferase, luciferase-expressing cells generate bioluminescence signals. This signal is detected by the in vivo imaging system and allows for a non-invasive means of monitoring tumor growth, distribution, and regression in individual animals. Drug administration via oral gavage allows for a maximum dosing volume of 10 mL/kg body weight to be delivered directly to the stomach and closely resembles delivery of drugs in clinical treatments. Therefore, techniques described here, development of an orthotopic mouse model of ovarian cancer, oral delivery of drugs, and in vivo imaging, are useful for better understanding of human ovarian cancer and treatment and will improve targeting this disease.

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.

活体成像和治疗卵巢癌的原位小鼠模型

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

生物学

Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy in the United States. Mortality is due to diagnosis of 75% of women with late stage disease, when metastasis is already present. EOC is characterized by diffuse and widely disseminated intra-peritoneal metastasis. Cells shed from the primary tumor anchor in the mesothelium that lines the peritoneal cavity as well as in the omentum, resulting in multi-focal metastasis, often in the presence of peritoneal ascites. Efforts in our laboratory are directed at a more detailed understanding of factors that regulate EOC metastatic success. However, quantifying metastatic tumor burden represents a significant technical challenge due to the large number, small size and broad distribution of lesions throughout the peritoneum. Herein we describe a method for analysis of EOC metastasis using cells labeled with red fluorescent protein (RFP) coupled with in vivo multispectral imaging. Following intra-peritoneal injection of RFP-labelled tumor cells, mice are imaged weekly until time of sacrifice. At this time, the peritoneal cavity is surgically exposed and organs are imaged in situ. Dissected organs are then placed on a labeled transparent template and imaged ex vivo. Removal of tissue auto-fluorescence during image processing using multispectral unmixing enables accurate quantitation of relative tumor burden. This method has utility in a variety of applications including therapeutic studies to evaluate compounds that may inhibit metastasis and thereby improve overall survival.

Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.

腹膜内卵巢癌转移的定量

Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy in the United States.  Mortality is due to diagnosis of 75% of ...

Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model

Source: Krishnan, V. et al. <a href="https://www.jove.com/t/52721">Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose.</a> J. Vis. Exp. (2015)
In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent metastasis.&#160;

腹腔注射细胞:一种将癌细胞输送到小鼠模型腹膜脂肪组织中的方法

Source: Krishnan, V. et al. Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose. J. Vis. Exp. 
...

原发肿瘤发展小鼠实验模型和腹膜转移

摘要

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此视频中的章节

浆液性卵巢癌原位模型在免疫小鼠，为在体内肿瘤显像和肿瘤免疫反应的监测

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

胫骨肉瘤细胞注射生成原位骨肉瘤和肺转移小鼠模型

小鼠模型的非侵入性成像检测和监测卵巢癌复发

基于人网膜的卵巢癌腹膜转移离体模型

基于人网膜的卵巢癌腹膜转移离体模型

基于人网膜的卵巢癌腹膜转移离体模型

活体成像和治疗卵巢癌的原位小鼠模型

腹膜内卵巢癌转移的定量

腹腔注射细胞:一种将癌细胞输送到小鼠模型腹膜脂肪组织中的方法

原发肿瘤发展小鼠实验模型和腹膜转移

摘要

探索更多视频

此视频中的章节

浆液性卵巢癌原位模型在免疫小鼠，为在体内肿瘤显像和肿瘤免疫反应的监测

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

胫骨肉瘤细胞注射生成原位骨肉瘤和肺转移小鼠模型

小鼠模型的非侵入性成像检测和监测卵巢癌复发

基于人网膜的卵巢癌腹膜转移离 体 模型

基于人网膜的卵巢癌腹膜转移离 体 模型

基于人网膜的卵巢癌腹膜转移离 体 模型

活体成像和治疗卵巢癌的原位小鼠模型

腹膜内卵巢癌转移的定量

腹腔注射细胞:一种将癌细胞输送到小鼠模型腹膜脂肪组织中的方法

基于人网膜的卵巢癌腹膜转移离体模型

基于人网膜的卵巢癌腹膜转移离体模型

基于人网膜的卵巢癌腹膜转移离体模型