Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

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Preparation of Biofilm Pellicles and Colonies

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Ethanol Resistance Assay

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Biofilm Colony Preparation for Scanning Electron Microscopy (SEM)

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Results: Bacillus subtilis Pellicle and Biofilm Formation Is Altered in the Presence of the Small Molecule Inhibitor D-leucine

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Conclusion