Use Artificial Substrates for Optimizing Enzyme Activities of (Phospho)lipases
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In Vivo Identification of the Physiological Substrate of a Lipase
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In Vitro Identification of the Physiological Substrate of Diacylglycerol (DAG) Lipase
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Results: Predicted Phospholipase SMc01003 Acts on Artificial p-Nitrophenyl Acyl Ester and DAG
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Conclusion
副本
The overall goal of this procedure is to accelerate the detection of physiological substrates for potential lipases and phosopholipases. This method can help answer key questions in the Entomology field, such as how phospholipases contribute to li
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Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.
