SBDS-FlAsH Tag Protein Expression and Purification
4:10
Elongation Factor Like-1 (EFL1) Protein Expression and Purification
7:07
Labeling of SBDS-FlAsH with the FlAsH Fluorescent Dye 4',5'-Bis(1,3,2 dithioarsolan-2-yl) Fluorescein and Fluorescence Anisotropy Experiments
8:50
Results: Evaluation of Protein-protein Interactions Using Fluorescence Anisotropy
9:55
Conclusion
Transcript
The overall goal of this experiment is to evaluate and quantify the interaction between two proteins of interest using Fluorescence Anisotropy. These measures can help answer key questions. The protein biochemistry and protein biophysics field, su
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Protein interactions are at the heart of a cell's function. Calorimetric and spectroscopic techniques are commonly used to characterize them. Here we describe fluorescence anisotropy as a tool to study the interaction between the protein mutated in the Shwachman-Diamond Syndrome (SBDS) and the Elongation factor-like 1 GTPase (EFL1).
