This protocol was originally published by Dr.Gary Bunker and his colleagues, and they allow for the long density and the high purity culture of embryonic loading hippocampal neurons, by suspending the neurons over a glial fitter layer. This result
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This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.
