Health Sciences Centre
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Low-Density Primary Hippocampal Neuron Culture
Reiko T. Roppongi *1,2, Kevin P. Champagne-Jorgensen *1,2, Tabrez J. Siddiqui 1,2
1Department of Physiology and Pathophysiology, University of Manitoba, 2Kleysen Institute for Advanced Medicine, Health Sciences Centre
This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.
Neuroscience
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
Noriko Koganezawa 1, Reiko T. Roppongi 2, Yuko Sekino 3,4, Izuo Tsutsui 3, Ayaka Higa 5, Tomoaki Shirao 1,5
1Department of Pharmacology, Graduate School of Medicine, Gunma University, 2Gunma University Initiative for Advanced Research, Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc.
A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate.
