March 27th, 2017
•Here we present a protocol to image cells expressing green fluorescent protein-tagged angiotensin type 1a receptors during endocytosis initiated by angiotensin II treatment. This technique includes labeling lysosomes with a second fluorescent marker, and then utilizing software to analyze the co-localization of receptor and lysosome in three dimensions over time.
Tags
Related Videos
Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations
Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
Analysis of SCAP N-glycosylation and Trafficking in Human Cells