Results: Representative AT1aR-GFP Expression and Analyses
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Conclusion
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The overall goal of this experiment is to obtain quantitative spatial and temporal evidence of Angiotensin type one receptor lysosome colocalization after Angiotensin two treatment. This method was devised to detect differences in the subcellular
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Here we present a protocol to image cells expressing green fluorescent protein-tagged angiotensin type 1a receptors during endocytosis initiated by angiotensin II treatment. This technique includes labeling lysosomes with a second fluorescent marker, and then utilizing software to analyze the co-localization of receptor and lysosome in three dimensions over time.