Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
5:15
Plasmid Recovery Using DNA Ligation and Bacterial Transformation
6:43
Results: Example Sequence of Assembled Gene
7:18
Conclusion
副本
This video demonstrates a procedure called, Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion or CAPRRESI, in which two chimeric genes are generated by introducing specific restriction enzyme sites into synonym DNA and the
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Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI), a protocol based on the insertion of restriction enzyme sites into synonym DNA sequences and functional plasmid recovery. This protocol is a fast and low-cost method for fusing protein-coding genes.
