In Vitro Generation of Caseum Surrogate from THP-1 Cells
5:23
Rapid Equilibration Dialysis (RED) Assay
8:02
LC-MS Quantification and Data Analysis
9:36
Representative RED Assay Results
11:27
Conclusion
副本
The overall goal of this protocol is to predict drug binding to caseum from tuberculous lesions. This method can help determine how effectively antimicrobials penetrate the caseus core of necrotic lesions without the use of animal models and expen
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Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.