Rutgers
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
Jansy P. Sarathy 1, Hsin-pin Ho Liang 1, Danielle Weiner 2, Jacqueline Gonzales 2, Laura E. Via 2, Véronique Dartois 1
1Public Health Research Institute Centre, New Jersey Medical School, Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH
Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.
JoVE Core
Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)
Matthew Zimmerman 1, Landry Blanc 1, Pei-Yu Chen 1, Véronique Dartois 1, Brendan Prideaux 1
1Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey
Here, we describe a protocol using laser capture microdissection coupled with LC/MS analysis to spatially-quantify drug distributions within pulmonary tuberculosis granulomas. The approach has broad applicability to quantifying drug concentrations within tissues at high spatial detail.
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