May 1st, 2017
•We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.
Related Videos
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
Miniaturized Sample Preparation for Transmission Electron Microscopy
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition