Induction of Hypoxia in Embryos and Collection of Tissue to Monitor HIF-1α Levels
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Incubation of Embryos in Hypoxia for EdU Incorporation
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Results: The Effects of Hypoxia on HIF-1α and on Cell Proliferation
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Conclusion
副本
The overall goal of this invention is to study the effects of hypoxia or hyperoxia on the embryonic tissues of any aquatic organism of interest. This method can help answer key questions in the field of developmental and cell biology, such as embr
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We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.