The overall goal of this standardized procedure is to isolate distinct male germ cell types from different mammalian species using a single protocol. This method can help facilitate the molecular study of spermatogenesis by the high-throughput ana
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This work describes the standardization of a method to obtain purified germ cell populations from testicular tissue of different mammalian species. It is a straightforward protocol that combines mechanical testis dissociation, staining with Hoechst-33342 and propidium iodide, and FACS sorting, with wide applications in comparative studies of male reproductive biology.