该研究得到了翻译健康科学研究所试点项目授予机会（UL1TR000423），华盛顿人类发展与残疾中心大学和华盛顿大学动物行为核心与脑成像核心的支持。我们要感谢Warren Ladiges博士在制定和传播这里提出的原始径向水面踏步迷宫设计和协议方面的作用。我们也感谢Toby Cole对这个项目的协助。

所有程序和动物处理均按照国家卫生研究院和华盛顿大学动物护理与使用委员会颁布的动物护理指南进行。 手术<ol><li>在诱导盒中麻醉5％异氟烷的老鼠，直到无意识。通过降低呼吸频率和在趾部夹紧后不存在撤回反射来确认麻醉。 </li><li>在手术过程中，通过鼻锥保持2-2.5％的麻醉。在整个手术过程中监测呼吸频率，以确保鼠标无意识。 </li><li>将鼠标放在加热垫上，并使用耳杆将鼠标放置在立体定向装置中，确保头部牢固且平整。 </li><li>使用脱毛霜从头皮上去除头发。用盐水彻底冲洗头皮。 </li><li>用交替碘和70％乙醇洗涤清洁手术部位。 </li><li> Administe在头皮皮下注射利多卡因和布比卡因（1mg / kg）。 </li><li>用手术剪刀做纵向中线切口，并收回皮肤以露出头骨。 </li><li>使用5毫米环钻预制件在左前额叶皮质上进行开颅手术，中心点在正前方2.5 mm处，中线左侧2.5 mm。小心地取出骨头露出大脑。 </li><li>将冲击器设备设置为6 m / s和200 ms驻留时间的速度。 </li><li>定位冲击器装置，直到3毫米凸出的撞击尖端轻轻地接触大脑后方2.5英寸后2.55毫米的中线。将冲击头缩回，并降低1 mm（冲击深度）。准备好后，启动设备，产生所需的影响。 </li><li>用无菌聚丙烯盘覆盖开颅手术，用组织粘合剂胶合到皮肤，并缝合切口。 </li><li>从麻醉中取出鼠标，并给予Bupr的IP注射（0.5mg / kg）。 </li><li>让鼠标在一个干净的笼子里恢复，加热垫加热。在接下来的24小时内，应该监测老鼠的疼痛或痛苦的迹象。 注意:假手术控制应该得到与上述相同的治疗，省略步骤1.8-1.9。 </li></ol>径向水踏面迷宫建筑<ol><li> Bore 9个出口孔，足够大以容纳1-1 / 2英寸ABS DWV SPG x SJ陷阱适配器，围绕32英寸直径镀锌钢桶的圆周等间隔。将这些出口孔围在浴缸地板的大约2-1 / 4英寸处。 </li><li>将1-1 / 2英寸ABS DWV SPG x SJ陷阱适配器安装到每个出口孔中，并使用附带的环形螺母固定。 </li><li>使用橡胶塞子，九口八口从设备外部出口。最后的未封顶出口将作为逃生路线。将退出指定为逃生路线无关紧要。 </li><li>安装90°ABS肘至剩余出口的外端。 90°弯曲用于防止测试对象从迷宫内部目视确定正确的逃生路线。 </li><li>从任何能够消毒的不透明盒子构成逃生箱，大小约为30厘米x 15厘米x 15厘米。在地板的正上方，在盒子的侧面切开一个孔，足够大以容纳90°的ABS弯头。 </li><li>将逃生箱安装在90°ABS弯头的末端。 </li><li>略微提升地板表面上方的逃生箱（小于一英寸）。这允许将电加热垫或其他加热源放置在逃逸箱下面的充足空间。 </li><li>打印和层压至少5个独特的视觉线索。使用简单，高对比度的图像，可以很容易地从设备中辨别出来。建议使用黑白剪贴画形状（三角形，正方形，圆形）。 </li><li>使用磁铁，将视觉线索粘贴到内壁仪器。围绕设备周围的距离应大致相等。 </li></ol>径向水踏面迷宫方案注意:水迷宫测试应仅在手术部位愈合后（术后约1周）开始。 <ol><li> 准备测试 <ol><li>允许小鼠在开始测试前至少30分钟适应测试室。 </li><li>使用70％乙醇喷雾消毒设备。 </li><li>用大约1英寸的冷（12-14℃）水填充设备。 </li><li>将电加热垫或其他加热源直接放在逃生箱下面。在整个测试期间保持逃生箱的黑暗和温暖。 </li><li>在设备上放置一个明亮的光源。 注意:如果使用从设备本身研究动物可能可见的灯，请注意确保灯泡处于同一波段每天都有一个意见灯本身可能代表了老鼠用于定位逃生箱的另一种视觉提示，并且每天都会大幅度地移动它可能会使结果复杂化。 </li></ol></li><li> 测试协议 <ol><li>将尾巴轻轻地从鼠笼中取出鼠标，放在设备的中央。 </li><li>一旦动物进入装置，开始计时。 </li><li>一旦动物找到正确的出口，并且已经找到/进入逃生箱，停止计时并记录找到正确路径所需的秒数。 </li><li>如果动物试图爬上一个终止孔，10秒后不会自动重入迷宫，用手将动物引回到迷宫的中心。 </li><li>如果动物未能在3分钟（180秒）内找到逃生箱的正确路径，则将该试验评定为失败记录为180秒。用手小心地将动物引导到正确的路径。 </li><li>允许小鼠留在逃生箱中1分钟，试用休息。 </li><li>一分钟的休息已经过去了，从逃生箱中取出动物并返回家中笼子。 </li><li>彻底消毒逃生箱，并用70％乙醇喷雾出口，以防止鼠标使用嗅觉线索找到正确的逃生路线。此步骤不应超过几秒钟。 </li><li>将鼠标放回迷宫进行下一次试验。 </li><li>重复步骤3.2.1-3.2.9，直到鼠标完成了三次试验，并已经定位或被引导到逃生箱三次。 </li><li>最后1分钟休息后，将鼠标移回家中的笼子。 </li><li>在动物之间排放并更换设备中的水，以确保整个测试过程中温度一致。 </li><li>对要测试的每只鼠标重复步骤3.2.1-3.2.12。 </li><li>第二天重复准备步骤3.2.1-3.2.5。特别注意确保视觉线索保留在测试日之间保持一致的位置。 </li><li>使用上述日常测试方案测试动物，每天进行三次试验，持续四天（训练期），第五天进行最后三次记忆保留试验。第十二个三年试验（长期记忆保留）可以在第十二天给予。 </li><li>在第五天和第十二天之间不要进行任何测试。 </li></ol></li><li> 分析 <ol><li>如果在任何两天的时间内，如果一只老鼠在180秒以内无法完成迷宫（ 即连续6次测试全部得分为180秒），请考虑由测试条件导致的鼠标不足，并从分析中删除。 </li><li>计算每个受试者完成迷宫的平均延迟时间，通过对其当天的三次日常试验进行平均化来测试日期。 </li><li>使用标准t检验在第五天测试和长期记忆测试日期比较组获得记忆保留的组差异。如果m比较两组比较，应采用单因素方差分析，然后进行适当的事后分析（如Tukey检验）跟踪所获得的任何显着性。 </li><li> 通过反复测量方差分析获得收购期（第1-4天）的组差异。 </li></ol></li></ol>

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</ol>

作者没有什么可以披露的。

这里介绍的RWT迷宫协议成功地区分了CCI诱导的TBI小鼠和假手术对照，代表了可行的，以鼠标为中心的替代MWM和Barnes圆形迷宫。而在这里报道的结果只会讲的使用在TBI小鼠模型中RWT迷宫，该设备已成功应用于老年人和转基因模型，其中应力诱导基于游泳测试导致违规使用MWM不切实际15,17国产，18，19。不?...

记忆障碍是由创伤性脑损伤（TBI）1，2名患者报告的最常见的症状之一。因此，准确识别和评估TBI动物模型中的类似记忆缺陷对于我们对这种状况及其管理的理解至关重要。在这里，我们提出了一个使用径向水胎（RWT）迷宫测试TBI鼠标模型中的空间记忆的协议。以前显示该装置可以评估受控皮层影响（CCI）诱导的TBI 3的小鼠模型中的认知缺陷，并且代表了大鼠验证的，基于游泳的认知测试的潜在替代方案。 转基因小鼠模型的日益多样化和可用性已经导致科研4使用小鼠大鼠上最近有所增加。尽管有这种转变，研究人员继续依靠行为最初为老鼠使用设计和验证的认知任务。目前用于评估在小鼠中的认知中最常见的测试，Morris水迷宫（MWM）和巴恩斯圆形迷宫，是专门设计，以利用大鼠5,6发现本能的行为。考虑到这两个物种4之间存在的遗传，神经行为和认知的差异，这是不足为奇的小鼠上这些任务7,8始终表现不佳。 测试能力方面的物种依赖性差异特别涉及基于游泳的认知测试，如MWM。虽然大鼠和小鼠是基于游泳认知任务9,10显着表现不佳精通游泳，研究人员已经确定了几个小鼠品系， </SUP&gt; 11，12，13。即使是在野生型动物，老鼠通常优于小鼠7,8。虽然这可以解释为空间记忆中的物种特异性差异，但使用干地迷宫的类似随访测试显示认知表现没有物种依赖性差异8 。与认知无关的一些因素可以解释这一发现，包括游泳能力或搜索策略中的物种依赖性差异。事实上，MWM中小鼠特异性搜索策略的因素分析表明，非空间因素（特别是Thigomotaxis和被动[ 即浮动]）可能在MWM表现中起到比空间学习更重要的作用14 。 在这里，我们展示了使用认知测试来设计来利用它小鼠的直觉行为，不需要游泳，测量CCI诱导的TBI小鼠模型中的空间记忆障碍。虽然RWT迷宫（ 图1 的A-B）被设想为在MWM和Barnes圆形迷宫的新颖混合型，它是专为采取的行为thigmotactic本能给小鼠15,16优势。该设备包括一个32英寸直径的镀锌钢桶，其中九个均匀分布的出口孔已经钻孔。孔位于浴缸底部的2-1 / 4英寸处，并且尺寸适合普通可用的1-1 / 2英寸ABS DWV SPG x SJ陷阱适配器。八个出口被外部盖住，并且用橡皮塞挡住1英寸的深度。第九个通过90°丙烯腈丁二烯苯乙烯（ABS）弯头连接到不透明的塑料盒上，测试后可以从中轻松取出鼠标。在一个过程中短暂采集期间，老鼠训练使用迷宫内的独特视觉线索找到这个逃生箱。在测试过程中，迷宫充满了一英寸冷水（12-14°C），足够的足够强大的逃逸能力，但不足以使鼠标游泳。 在RWT迷宫表示一种低成本，低维护的替代MWM，并已在老年和转基因小鼠15，17，18，19成功地使用，和CCI诱导的TBI 3的小鼠模型。这里概述的方案代表了一种测量空间记忆障碍的简单有效的方法，不需要进行伤前训练，可以轻松修改以适应研究实验室的特殊需要。

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assessing spatial memory impairment in a mouse model of traumatic brain injury using a radial water tread maze

尽管近来在科学研究中使用小鼠模型的增加，研究人员继续使用最初设计和验证大鼠使用的认知任务。以前已经显示了空间记忆的径向水胎（RWT）迷宫测试（专为小鼠而设计，不需要游泳），以成功区分受控的皮层撞击诱导的TBI小鼠和假手术对照。在这里，介绍了此任务的详细协议。 RWT迷宫利用小鼠自然倾向避免开放区域，有利于拥抱设备的两侧（thigmotaxis）。迷宫的墙壁上装有九个放置在设备地板上的逃生孔，老鼠训练使用视觉线索来定位引出迷宫的逃生孔。迷宫充满了一英寸的冷水，足以激发逃脱，但不够深，要求鼠标游泳。收购期仅需四次天，在第5天进行记忆保留测试，并在第12天进行长期记忆测试。这里报告的结果表明，RWT迷宫是评估空间中大鼠验证的，基于游泳的认知测试的可行替代方案TBI小鼠模型的记忆缺陷。

RWT迷宫（ 图1 ）用于研究随机分配接受受控皮质冲击诱导的TBI或假手术的小鼠中损伤依赖性空间记忆缺陷。使用电磁驱动的皮质冲击产生损伤，其具有3mm的凸尖，并且以下损伤参数:6m / s的冲击速度，1mm的穿透深度和200ms的接触时间。小鼠从手术后35天开始接受认知测试，并进行了四天的培训（收购期），然后在第5天进行记忆保留测试，并在第12天进?...

Watch this Scientific Journal Video about Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze at JoVE.com

Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze

在这里，我们提出了一个不需要游泳的认知的小鼠特异性测试方案。该测试可用于成功地区分受控皮质冲击引起的创伤性脑损伤小鼠与假手术对照。

使用径向水痕迷宫评估外伤性脑损伤小鼠模型中的空间记忆障碍

Despite the recent increase in use of mouse models in scientific research, researchers continue to use cognitive tasks that were originally designed and ...

assessing-spatial-memory-impairment-mouse-model-traumatic-brain

Research

JoVE Journal

Behavior

7.1K 次观看.  University of Washington. 在这里，我们提出了一个不需要游泳的认知的小鼠特异性测试方案。该测试可用于成功地区分受控皮质冲击引起的创伤性脑损伤小鼠与假手术对照。 

视频: Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze

Use of an Eight-arm Radial Water Maze to Assess Working and Reference Memory Following Neonatal Brain Injury

Working and reference memory are commonly assessed using the land based radial arm maze.&#160;However, this paradigm requires pretraining, food deprivation, and may introduce scent cue confounds. The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs. Specifically, subjects are required to avoid the arms previously used for escape during each testing day (working memory) as well as avoid the fixed arms, which never contain escape platforms (reference memory). Re-entries into arms that have already been used for escape during a testing session (and thus the escape platform has been removed) and re-entries into reference memory arms are indicative of working memory deficits. Alternatively, first entries into reference memory arms are indicative of reference memory deficits. We used this maze to compare performance of rats with neonatal brain injury and sham controls following induction of hypoxia-ischemia and show significant deficits in both working and reference memory after eleven days of testing. This protocol could be easily modified to examine many other models of learning impairment.

The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs.

Working and reference memory are commonly assessed using the land based radial arm maze.&#160;However, this paradigm requires pretraining, food ...

科研

行为学

Quantifying Cognitive Decrements Caused by Cranial Radiotherapy

With the exception of survival, cognitive impairment stemming from the clinical management of cancer is a major factor dictating therapeutic outcome. For many patients afflicted with CNS and non-CNS malignancies, radiotherapy and chemotherapy offer the best options for disease control. These treatments however come at a cost, and nearly all cancer survivors (~11 million in the US alone as of 2006) incur some risk for developing cognitive dysfunction, with the most severe cases found in patients subjected to cranial radiotherapy (~200,000/yr) for the control of primary and metastatic brain tumors1. Particularly problematic are pediatric cases, whose long-term survival plagued with marked cognitive decrements results in significant socioeconomic burdens2. To date, there are still no satisfactory solutions to this significant clinical problem.
We have addressed this serious health concern using transplanted stem cells to combat radiation-induced cognitive decline in athymic rats subjected to cranial irradiation3. Details of the stereotaxic irradiation and the in vitro culturing and transplantation of human neural stem cells (hNSCs) can be found in our companion paper (Acharya et al., JoVE reference). Following irradiation and transplantation surgery, rats are then assessed for changes in cognition, grafted cell survival and expression of differentiation-specific markers 1 and 4-months after irradiation. To critically evaluate the success or failure of any potential intervention designed to ameliorate radiation-induced cognitive sequelae, a rigorous series of quantitative cognitive tasks must be performed. To accomplish this, we subject our animals to a suite of cognitive testing paradigms including novel place recognition, water maze, elevated plus maze and fear conditioning, in order to quantify hippocampal and non-hippocampal learning and memory. We have demonstrated the utility of these tests for quantifying specific types of cognitive decrements in irradiated animals, and used them to show that animals engrafted with hNSCs exhibit significant improvements in cognitive function3.
The cognitive benefits derived from engrafted human stem cells suggest that similar strategies may one day provide much needed clinical recourse to cancer survivors suffering from impaired cognition. Accordingly, we have provided written and visual documentation of the critical steps used in our cognitive testing paradigms to facilitate the translation of our promising results into the clinic.

Cognitive impairment resulting from the radiotherapeutic management of brain tumors represents a clinically intractable condition that adversely impacts quality of life. The capability to critically evaluate potential interventions for ameliorating radiation-induced cognitive decrements ultimately depends on the capability to undertake rigorous quantitative assessments of cognition.

量化颅放疗引起的认知递减

With the exception of survival, cognitive impairment stemming from the clinical management of cancer is a major factor dictating therapeutic outcome. For ...

医学

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer&#39;s Disease Model Mice

The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including acquisition of spatial memoryand long-term spatial memory 1. The MWM is a relatively simple procedure typically consisting of six day trials, the main advantage being the differentiation between the spatial (hidden-platform) and non-spatial (visible platform) conditions 2-4. In addition, the MWM testing environment reduces odor trail interference 5. This has led the task to be used extensively in the study of the neurobiology and neuropharmacology of spatial learning and memory. The MWM plays an important role in the validation of rodent models for neurocognitive disorders such as Alzheimer&#x2019;s Disease 6, 7. In this protocol we discussed the typical procedure of MWM for testing learning and memory and data analysis commonly used in Alzheimer&#x2019;s disease transgenic model mice.

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer's Disease Model Mice

The Morris Water Maze is a behavioral task to test hippocampal-dependent learning and memory. It has been widely used in the study of neurobiology, neuropharmacology and neurocognitive disorders in rodent models.

在阿尔茨海默氏病模型小鼠学习和记忆障碍的Morris水迷宫测试

The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including ...

神经科学

Utilizing the Modified T-Maze to Assess Functional Memory Outcomes After Cardiac Arrest

Background: Evaluating mild to moderate cognitive impairment in a global cerebral ischemia (i.e. cardiac arrest) model can be difficult due to poor locomotion after surgery. For example, rats who undergo surgical procedures and are subjected to the Morris water maze may not be able to swim, thus voiding the experiment.
New Method: We established a modified behavioral spontaneous alternation T-maze test. The major advantage of the modified T-maze protocol is its relatively simple design that is powerful enough to assess functional learning/memory after ischemia. Additionally, the data analysis is simple and straightforward. We used the T-maze to determine the rats&#39; learning/memory deficits both in the presence or absence of mild to moderate (6 min) asphyxial cardiac arrest (ACA). Rats have a natural tendency for exploration and will explore the alternate arms in the T-maze, whereas hippocampal-lesioned rats tend to adopt a side-preference resulting in decreased spontaneous alternation ratios, revealing the hippocampal-related functional learning/memory in the presence or absence of ACA.
Results: ACA groups have higher side-preference ratios and lower alternations as compared to control.
Comparison with Existing Method(s): The Morris water and Barnes maze are more prominent for assessing learning/memory function. However, the Morris water maze is more stressful than other mazes. The Barnes maze is widely used to measure reference (long-term) memory, while ACA-induced neurocognitive deficits are more closely related to working (short-term) memory.
Conclusions: We have developed a simple, yet effective strategy to delineate working (short-term) memory via the T-maze in our global cerebral ischemia model (ACA).

This protocol describes the use of a modified T-maze to evaluate functional learning/memory in asphyxia cardiac arrest-induced cerebral ischemia.

应用改良 T 迷宫评估心脏骤停后的功能记忆结果

Background: Evaluating mild to moderate cognitive impairment in a global cerebral ischemia (i.e. cardiac arrest) model can be difficult due to poor ...

A Behavioral Assay for Investigating the Role of Spatial Memory During Instinctive Defense in Mice

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often stereotyped actions elicited in response to innately aversive sensory stimuli, but their success requires enough flexibility for adapting to different spatial environments, which can change rapidly. Here, we describe a behavioral assay to evaluate the influence of learned spatial knowledge on defensive behaviors in mice. We have adapted the widely used Barnes maze spatial memory assay to investigate how mice navigate to a shelter during escape responses to innately aversive sensory stimuli in a novel environment, and how they adapt to acute changes in the environment. This new assay is an ethological paradigm that does not require training and exploits the natural exploration patterns and navigation strategies in mice. We propose that the set of protocols described here are a powerful means of studying goal-directed behaviors and stimulus-triggered navigation, which should be of interest to both the fields of instinctive behaviors and spatial memory.

This protocol describes a behavioral assay, based on the Barnes maze test, to study how instinctive defensive actions are modified by the knowledge of spatial environment.

一种研究空间记忆在小鼠本能防御中作用的行为分析法

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often ...

Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze

A Morris water maze (MWM) experiment forces experimental animals to swim and learn to find a platform hidden in the water. It is widely used in scientific research to assess the learning and memory of animals. Due to the extensive use of the MWM test, visual experimental protocols are essential for researchers. This manuscript uses the latest studies to introduce the protocol of the MWM test. Alzheimer&#8217; Disease (AD) is characterized by a progressive loss of memory and cognitive function. An alternative and complementary treatment used for AD is Manual Acupuncture (MA). To assess the learning and memory ability of AD model mice, the MWM test was conducted. The visible platform trial, hidden platform trial, probe trial, and reversal trial of MWM were used to evaluate spatial learning and memory ability. In the visible platform trial, the swimming speed and escape latency of mice in different groups was not significantly different. In the hidden platform and reversal trials, the AD group showed a long escape latency. The escape latency decreased significantly after the MA treatment. Low platform crossover number and the proportion of time in the SW quadrant in the probe trial increased after the MA treatment (p &#60; 0.05 or p &#60; 0.01). The results of the MWM tests suggest that MA can effectively improve the spatial learning and memory abilities of AD model mice. Rigorous experimental operations provided assurance of the reliability of the results.

Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer&#8217;s Disease model mice and to assess the effect of manual acupuncture for treating them is described.

使用莫里斯水迷宫对阿尔茨海默氏病小鼠模型学习和记忆能力的分析

A Morris water maze (MWM) experiment forces experimental animals to swim and learn to find a platform hidden in the water. It is widely used in scientific ...

Motor and Hippocampal Dependent Spatial Learning and Reference Memory Assessment in a Transgenic Rat Model of Alzheimer's Disease with Stroke

Alzheimer&#39;s disease (AD) is a debilitating neurodegenerative disease that results in neurodegeneration and memory loss. While age is a major risk factor for AD, stroke has also been implicated as a risk factor and an exacerbating factor. The co-morbidity of stroke and AD results in worsened stroke-related motor control and AD-related cognitive deficits when compared to each condition alone. To model the combined condition of stroke and AD, a novel transgenic rat model of AD, with a mutated form of amyloid precursor protein (a key protein involved in the development of AD) incorporated into its DNA, is given a small unilateral striatal stroke.
For a model with the combination of both stroke and AD, behavioral tests that assess stroke-related motor control, locomotion and AD-related cognitive function must be implemented. The cylinder task involves a cost-efficient, multipurpose apparatus that assesses spontaneous forelimb motor use. In this task, a rat is placed in a cylindrical apparatus, where the rat will spontaneously rear and contact the wall of the cylinder with its forelimbs. These contacts are considered forelimb motor use and quantified during video analysis after testing. Another cost-efficient motor task implemented is the beam-walk task, which assesses forelimb control, hindlimb control and locomotion. This task involves a rat walking across a wooden beam allowing for the assessment of limb motor control through analysis of forelimb slips, hindlimb slips and falls. Assessment of learning and memory is completed with Morris water maze for this behavioral paradigm. The protocol starts with spatial learning, whereby the rat locates a stationary hidden platform. After spatial learning, the platform is removed and both short-term and long-term spatial reference memory is assessed. All three of these tasks are sensitive to behavioral differences and completed within 28 days for this model, making this paradigm time-efficient and cost-efficient.

To investigate the co-morbid Alzheimer&#39;s disease (AD) and stroke condition in a novel model, three behavior tasks are described that assess both motor control and cognitive behaviors. These tasks include the beam-walk task, cylinder task and Morris water maze.

汽车和海马依赖空间学习和记忆参考评估与中风阿尔茨海默氏病的转基因大鼠模型

Alzheimer&#39;s disease (AD) is a debilitating neurodegenerative disease that results in neurodegeneration and memory loss. While age is a major risk ...

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of human studies, animal models provide suitable alternatives to test treatment methods, and to study the mechanisms and related complications of the condition. Experimental rodent models have historically been the most widely used due to their accessibility, low cost, reproducibility, and validated approaches. A metric test, which tests the ability to recall the placement of two objects at various distances and angles from one another, is a technique to study impairment in spatial working memory (SWM) after TBI. The significant advantages of metric tasks include the possibility of dynamic observation, low cost, reproducibility, relative ease of implementation, and low stress environment. Here, we present a metric test protocol to measure impairment of SWM in adult rats after TBI. This test provides a feasible way to evaluate physiology and pathophysiology of brain function more effectively.

Traumatic brain injury (TBI) is commonly associated with memory impairment. Here, we present a protocol to assess spatial working memory after TBI via a metric task. A metric test is a useful tool to study spatial working memory impairment after TBI.

创伤性脑损伤后评估成年大鼠空间工作记忆的指标测试

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of ...

通过闭合头部损伤法在小鼠中诱导 rmTBI

Modified Mouse Model of Repetitive Mild Traumatic Brain Injury Incorporating Thinned-Skull Window and Fluid Percussion

Mild traumatic brain injury is a clinically highly heterogeneous neurological disorder. Highly reproducible traumatic brain injury (TBI) animal models with well-defined pathologies are urgently needed for studying the mechanisms of neuropathology after mild TBI and testing therapeutics. Replicating the entire sequelae of TBI in animal models has proven to be a challenge. Therefore, the availability of multiple animal models of TBI is necessary to account for the diverse aspects and severities seen in TBI patients. CHI is one of the most common methods for fabricating rodent models of rmTBI. However, this method is susceptible to many factors, including the impact method used, the thickness and shape of the skull bone, animal apnea, and the type of head support and immobilization utilized. The aim of this protocol is to demonstrate a combination of the thinned-skull window and fluid percussion injury (FPI) methods to produce a precise mouse model of CHI-associated rmTBI. The primary objective of this protocol is to minimize factors that could impact the accuracy and consistency of CHI and FPI modeling, including skull bone thickness, shape, and head support. By utilizing a thinned-skull window method, potential inflammation due to craniotomy and FPI is minimized, resulting in an improved mouse model that replicates the clinical features observed in patients with mild TBI. Results from behavior and histological analysis using hematoxylin and eosin (HE) staining suggest that rmTBI can lead to a cumulative injury that produces changes in both behavior and gross morphology of the brain. Overall, the modified CHI-associated rmTBI presents a useful tool for researchers to explore the underlying mechanisms that contribute to focal and diffuse pathophysiological changes in rmTBI.

作者聚焦：优化啮齿动物模型以研究反复轻度创伤性脑损伤的机制和康复

This protocol presents a modified mouse model of repetitive mild traumatic brain injury (rmTBI) induced via a closed-head injury (CHI) method. The approach features a thinned-skull window and fluid percussion to reduce the inflammation commonly caused by meninges exposure, along with improved reproducibility and accuracy in modeling rmTBI in rodents.

结合颅骨窗变薄和液体叩击的重复性轻度创伤性脑损伤改良小鼠模型

Mild traumatic brain injury is a clinically highly heterogeneous neurological disorder. Highly reproducible traumatic brain injury (TBI) animal models ...

使用径向水痕迷宫评估外伤性脑损伤小鼠模型中的空间记忆障碍

摘要

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