Image Acquisition of Multi-color Beads and Golgi Mini-stacks
4:36
Image Analysis, Acquire Centers of Fluorescent Beads
7:57
Select and Measure Golgi Mini-stacks
10:59
Results: Imaging the Golgi's Center of Fluorescence Mass
12:31
Conclusion
副本
The overall goal of this imaging-based method is to quantitatively localize a golgi protein at nanometer resolution. This method can help to answer the key questions in the study of the golgi apparatus such as the golgi's organization and how it w
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The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.