Analysis of the Distribution of Organelles in B Cells Activated with Ag-coated Beads
7:42
Analysis of Cell Spreading and Distribution of Organelles in B Cells Activated on Ag-coated Coverslips
9:29
Isolation of Centrosome-enriched Fractions from Resting and Activated B Cells
11:23
Results
15:07
Conclusion
副本
In this review, we will discuss both imaging and biochemical techniques used to study organelle positioning and composition, as well as cytoskeleton rearrangements observed during the formation of an immunological synapse. Initial stages of active
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Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
