Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
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Conclusion
副本
The overall goal of this protocol is to provided viewers with detailed procedures to produce Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex. This PN injection protocol we developed in the Golden Sy
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Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.