Slicing the Retina and Assembling the Imaging Ladder
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Results: Visualizing Ca2-dynamics in Cone Photoreceptors
10:19
Conclusion
Transcript
The overall goal of this procedure is to prepare fresh slices of Zebrafish retinas for experiments that require live imaging of fluorescent proteins or biosensors. This method can help answer key questions in the field of vision research, such as
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Imaging retinal tissue can provide single-cell information that cannot be gathered from traditional biochemical methods. This protocol describes preparation of retinal slices from zebrafish for confocal imaging. Fluorescent genetically encoded sensors or indicator dyes allow visualization of numerous biological processes in distinct retinal cell types.
