Sample Collection and Preparation for Histological Staining and Slides
5:09
Hematoxin & Eosin Staining
6:07
Periodic Acid-Schiff Staining
7:19
Sample Collection and Preparation for RNA Extraction
8:30
Results: Histological Analysis of C. albicans Infection in Mice
10:00
Conclusion
Transcript
The overall goal of this procedure is to observe the activation of the immune system during skin infections by injecting Candida albicans to preserve skin architecture and the localization of specific immune and non-immune cells. This method can h
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Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.
