Results: ATP Puffing by Micro-pipette Induces Vessel Dilation, followed by Constriction
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Conclusion
副本
This protocol is designed to study the slow conducted vascular responses in cerebral capillaries elicited by local ejection of ATP.The advantage of this technique is to simulate local synaptic activities by local pipette ejection and to keep the s
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We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.