High Precision Coverslip and Glass Slide Preparation
1:17
Fluorescent Microsphere and Spore Sample Mounting
2:32
Fluorescent Microsphere and Spore Imaging
3:38
FM4-64 Stained PS4150 Spore 3D-SIM Raw Image Reconstruction
5:00
Image Analysis
6:25
Results: Representative Germinosome and Inner Membrane Visualization
8:04
Conclusion
副本
Super-resolution structured illumination microscopy, 3D-SIM, is an innovative technique for the visualization of fluorescent reporter proteins of germination receptor clusters in spore membranes. Using these procedures, unsurpassed resolution of g
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Germinant receptor proteins cluster in ‘germinosomes’ in the inner membrane of Bacillus subtilis spores. We describe a protocol using super resolution microscopy and fluorescent reporter proteins to visualize germinosomes. The protocol also identifies spore inner membrane domains that are preferentially stained with the membrane dye FM4-64.