Time-Lapse Image Acquisition and Swarm Repulsion Measurement
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Results: Representative Time-lapse Swarming and Collective Stress Response Imaging
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Conclusion
副本
This protocol can be used to produce time-lapse movies of Pseudomonas aeruginosa swarming and to capture the repulsion of swarm by bacteriophage or antibiotics. The time-lapse movies capture the detailed dynamics of the swarming response to antibi
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We detail a simple method to produce high-resolution time-lapse movies of Pseudomonas aeruginosa swarms that respond to bacteriophage (phage) and antibiotic stress using a flatbed document scanner. This procedure is a fast and simple method for monitoring swarming dynamics and may be adapted to study the motility and growth of other bacterial species.
