Name: acupuncture in a rat model of asthma
Uploaded: 2020-08-25T15:00:00.000+00:00
Duration: 7 min 14 s
Description: Watch this Scientific Journal Video about Acupuncture in a Rat Model of Asthma at JoVE.com

这项工作得到了中国国家自然科学基金委员会（第81922076号、81973951号、81873373号）、上海市合作创新建设项目（第81922076号）的支持。ZYJKFW201811010，ZYJKFW201701005）。

所有动物实验均经上海中医药大学动物实验伦理委员会审核通过（伦理许可号PZSHUTCM190308020）。
1. 高平台的制造
<ol><li>将支撑装置的长度和宽度设置为 86 厘米 x 17 厘米（图 1）。</li>
	<li>垂直修复连接设备以连接到支持设备。连接装置应长约30厘米，以防止大鼠跑到高平台的顶部表面，宽度为14厘米。</li>
	<li>将水平踏板（5 厘米 x 5 厘米）固定到连接设备上。水平踏板底部的悬浮高度应大于或等于 50 厘米。 注:根据实验鼠的数量，可以使用多个水平踏板，每个踏板上放置一只大鼠。</li>
</ol>2. 建立哮喘的老鼠模型
<ol><li>用 OVA 使大鼠敏感<ol>
<li>在第 0 天准备感化解决方案。用1毫克OVA和10毫克氢氧化铝准备每1毫升的感化溶液，以普通盐水作为溶剂。</li>
		<li>在第0天给每只大鼠注射1mL的敏感溶液。使用合适的注射器绘制感性解决方案，供以后使用。</li>
		<li>抓住尾巴，抬起老鼠，放在粗糙的表面上，轻轻地把尾巴拉回来。用左拇指和食指抓住耳朵和脖子的皮肤，同时用剩下的三根手指和手掌紧紧握住背部皮肤（小心不要施加会窒息老鼠的过度力）。</li>
		<li>用右手，用1mL的感官溶液准备注射器，在大鼠中进行腹膜内注射。</li>
	</ol>
</li>
	<li>挑战大鼠与OVA<ol>
<li>选择带盖子的盒子（长度、宽度和高度为 39 厘米 x 28 厘米 x 23 厘米），并在侧面钻一个小孔。用橡胶管连接密封的盒子、雾化杯和压缩雾化器（补充图1）。 注:压缩雾化器的星云粒子大小为1-5微米，雾化率大于0.2 mL/min，最终压力为205 kPa。</li>
		<li>在敏感后的第14天，打开自制密封盒的盖子，将老鼠放在盒子里（盒子可以容纳5只老鼠），然后把密封的盒子放在一个生物安全柜里。</li>
		<li>准备挑战解决方案。将300毫克OVA与30毫升普通盐水彻底混合。</li>
		<li>打开超声波雾化器，将 30 mL 的挑战解决方案添加到雾化杯中。根据自制密封盒的体积调整挑战溶液的剂量和大鼠数量。</li>
		<li>重复数次（每天一次）或根据哮喘症状的严重程度调整程序。</li>
	</ol>
</li>
</ol>3. 针灸治疗
<ol><li>根据治疗哮喘的中医理论，选择包括GV14、双边BL12和双边BL13在内的穴位（补充图2）。在第2天进行手动针灸，每隔一天进行一次，共7次（图2）。
	<ol>
<li>针灸GV14位于第七颈椎和第一胸椎（肩骨的空心）之间，在背部中线（图3）。</li>
		<li>针灸 BL13 位于第三个胸椎以下，位于腹腔间空间的两侧（肩骨的下边缘）。</li>
		<li>针灸 BL12 位于第二个胸椎以下，位于间歇空间的两侧（在肩骨下方，根据 BL13 确定的位置）。</li>
	</ol>
</li>
	<li>抓住老鼠，把它们放在高高的平台上。</li>
	<li>用右手拇指和食指握住一次性不锈钢针（0.3毫米 x 13.0 毫米， 辅助图 3）手柄，用右手手掌按鼠背。 注:一次性针头必须仅一次性使用。因为反复使用会使针头钝化，从而增加大鼠的疼痛。</li>
	<li>触摸上边缘 （GV14） 或肩骨下边缘 （BL12 和 BL13） 中间的凹陷，然后用针尖轻轻刺破大鼠的皮肤以定位穴位。</li>
	<li>确定穴位的具体位置后，快速插入针头，并迅速移除右手。</li>
	<li>均匀地扭动针头约360°，速度为60次/分钟，10-20秒，每5分钟操纵一次，然后在20-30分钟后取出（补充图4）。</li>
</ol>4. 笔测量
<ol><li>准备甲基苯甲基苯甲酰:将100毫克甲基胆碱放入5mL离心管中，并将PBS添加到2mL（50毫克/mL甲基苯甲酰胆碱）的体积中。然后，将 50 毫克/mL 甲基胆碱溶液稀释到 25 毫克/mL、12.5 毫克/mL、6.25 毫克/mL 和 3.125 毫克/mL。</li>
	<li>仪器检查<ol>
<li>打开信号放大器（气溶胶分布系统和啮齿动物偏置流供应系统全部关闭，星云连接器关闭）。</li>
		<li>检查通道:单击 校准，将粗调整基线返回到 0，然后切换到直流 （DC） 位置。将基线微调到 0，用注射器注入 5 mL 气体。然后，切换到交替电流 （AC） 位置。</li>
		<li>检查通道后，确保误差值小于 0.5%。</li>
	</ol>
</li>
	<li>设置参数<ol>
<li>打开气溶胶分布系统和啮齿动物偏置流供应系统。根据汞球上的液体水平将流速调整到 4.5 mL。</li>
		<li>点击 创建研究选项 ，并选择 剂量响应研究，选择 老鼠 的物种，并点击 下一步。</li>
		<li>选择 简单的剂量列表，将 剂量数 设置为 6， 然后单击 下一个。</li>
		<li>选择 Penh 和 区域 参数:将动物适应时间设置为 5 分钟，雾化时间设置为 2 分钟，响应时间设置为 3 分钟，恢复时间设置为 2 分钟。</li>
		<li>依次向雾化器添加以下解决方案（每只大鼠 200 μL）:甲基胆碱（0 毫克/mL、3.125 毫克/mL、6.25 毫克/mL、12.5 毫克/mL、25 毫克/mL 和 50 毫克/mL）。可以观察到 Penh 的实时变化 （图 4）。</li>
		<li>在响应所有 6 种不同浓度的解决方案后，单击 "文件 "和 "结束会话 "以完成实验。</li>
	</ol>
</li>
</ol>

<ol>
	<li>Papi, A., Brightling, C., Pedersen, S. E., Reddel, H. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Asthma.">Asthma.</a> Lancet. 391 (10122), 783-800 (2018).</li><li>. Global strategy for asthma management and prevention Available from: <a target="_blank" href="https://ginasthma.org/gina-reports/">https://ginasthma.org/gina-reports/</a> (2019)</li><li>Enilari, O., Sinha, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Global+Impact+of+Asthma+in+Adult+Populations.">The Global Impact of Asthma in Adult Populations.</a> Annals of Global Health. 85 (1), (2019).</li><li>. Global strategy for asthma management and prevention Available from: <a target="_blank" href="https://ginasthma.org/gina-reports/">https://ginasthma.org/gina-reports/</a> (2020)</li><li>Zhuang, Y., Xing, J. J., Li, J., Zeng, B. Y., Liang, F. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=History+of+acupuncture+research.">History of acupuncture research.</a> International Review of Neurobiology. 111, 1-23 (2013).</li><li>Yang, Y. Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Considerations+for+use+of+acupuncture+as+supplemental+therapy+for+patients+with+allergic+asthma.">Considerations for use of acupuncture as supplemental therapy for patients with allergic asthma.</a> Clinical Reviews in Allergy &amp; Immunology. 44 (3), 254-261 (2013).</li><li>Nurwati, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduction+of+interleukin-17+level+by+acupuncture+at+Feishu+(BL13)+is+strengthened+by+acupuncture+at+Zusanli+(ST36)+in+a+mouse+model+of+chronic+asthma:+An+experimental+study.">Reduction of interleukin-17 level by acupuncture at Feishu (BL13) is strengthened by acupuncture at Zusanli (ST36) in a mouse model of chronic asthma: An experimental study.</a> Medical Acupuncture. 27 (4), 278-282 (2015).</li><li>Nurwati, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improvement+in+inflammation+and+airway+remodelling+after+acupuncture+at+BL13+and+ST36+in+a+mouse+model+of+chronic+asthma.">Improvement in inflammation and airway remodelling after acupuncture at BL13 and ST36 in a mouse model of chronic asthma.</a> Acupuncture in Medicine. 37 (4), 228-236 (2019).</li><li>Reddy, A. T., Lakshmi, S. P., Reddy, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+model+of+allergen+induced+asthma.">Murine model of allergen induced asthma.</a> Journal of Visualized Experiments. (63), e3771 (2012).</li><li>Zhou, D. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metallothionein-2+is+associated+with+the+amelioration+of+asthmatic+pulmonary+function+by+acupuncture+through+protein+phosphorylation.">Metallothionein-2 is associated with the amelioration of asthmatic pulmonary function by acupuncture through protein phosphorylation.</a> Biomedicine &amp; Pharmacotherapy. 123, 109785 (2020).</li><li>Yin, L. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transgelin-2+as+a+therapeutic+target+for+asthmatic+pulmonary+resistance.">Transgelin-2 as a therapeutic target for asthmatic pulmonary resistance.</a> Science Translational Medicine. 10 (427), (2018).</li><li>Shin, Y. S., Takeda, K., Gelfand, E. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+asthma+using+animal+models.">Understanding asthma using animal models.</a> Allergy Asthma &amp; Immunology Research. 1 (1), 10-18 (2009).</li><li>Cui, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electro-acupuncture+relieves+chronic+visceral+hyperalgesia+in+rats.">Electro-acupuncture relieves chronic visceral hyperalgesia in rats.</a> Neuroscience Letters. 376 (1), 20-23 (2005).</li><li>Tobin, J. R., Martin, L. D., Breslow, M. J., Traystman, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+anesthetic+inhibition+of+brain+nitric+oxide+synthase.">Selective anesthetic inhibition of brain nitric oxide synthase.</a> Anesthesiology. 81 (5), 1264-1269 (1994).</li><li>Dimitrov, N., Sivrev, D., Atanasova, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Method+of+rat+immobilization+during+experimental+acupuncture.">Method of rat immobilization during experimental acupuncture.</a> Scripta Scientifica Medica. 45, 105-108 (2013).</li><li>Aguilar, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=emotional+reactivity+and+fear+of+heights:+a+factor+analytic+map+from+a+large+F(2)+intercross+of+Roman+rat+strains.">emotional reactivity and fear of heights: a factor analytic map from a large F(2) intercross of Roman rat strains.</a> Brain Research Bulletin. 57 (2), 17-26 (2002).</li><li>Lai, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proteomic+response+to+acupuncture+treatment+in+spontaneously+hypertensive+rats.">Proteomic response to acupuncture treatment in spontaneously hypertensive rats.</a> PloS One. 7 (9), 44216 (2012).</li><li>Niu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+uni-acupoint+electroacupuncture+stimulation+method+for+pain+relief.">A novel uni-acupoint electroacupuncture stimulation method for pain relief.</a> Evidence-Based Complementary and Alternative Medicine. 2011, 209879 (2011).</li><li>McFadden, E. R., Luparello, T., Lyons, H. A., Bleecker, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+mechanism+of+action+of+suggestion+in+the+induction+of+acute+asthma+attacks.">The mechanism of action of suggestion in the induction of acute asthma attacks.</a> Psychosomatic Medicine. 31 (2), 134-143 (1969).</li><li>Savov, J. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ozone-induced+acute+pulmonary+injury+in+inbred+mouse+strains.">Ozone-induced acute pulmonary injury in inbred mouse strains.</a> American Journal of Respiratory Cell and Molecular Biology. 31 (1), 69-77 (2004).</li></ol>

作者没有什么可透露的。

本研究中使用的GV14、双边BL12和双边BL13穴位位于背面，因此高平台适合固定大鼠。在其他研究中，应根据穴位15的位置选择适当的固定方法。对于腹部的穴位，大鼠可以向下放置在一个黑色的圆锥体（类似于面包师使用的糕点袋）17。链条连接到约束锥固定，大鼠的后腿也固定。这样，动物身体的前部被牢牢地固定在圆锥体中，而腹部和背部则暴露在外。对于腿...

哮喘是一种慢性疾病，其特征是支气管收缩和气道反应过度。全世界约有3.394亿人被诊断患有哮喘2。全球哮喘患病率正在上升，预计未来十年将新增1亿例。吸入皮质类固醇和长效β 2-agonists不能有效地控制所有患者的症状4。因此，使用针灸等替代疗法治疗哮喘已受到越来越多的关注。治疗哮喘的有效方法是针GV14（大泽），双边BL12（丰门）和双边BL13（飞舒）6。针灸显著降低过敏性哮喘患者唾液和鼻分泌物中分泌免疫球蛋白A（SIgA）的水平，减少周围血液中嗜血球菌的计数，总有效率为85%6。慢性哮喘小鼠的BL13和ST36（Zusanli）针灸可降低IL-17水平40%，与椭圆形布明（OVA）组相比，平滑肌肉厚度降低33%，从而缓解炎症症状7、8。然而，哮喘针灸治疗的机制并不完全清楚。
动物模型是哮喘研究的重要工具9，因为动物模型可用于持续测量呼吸功能，以评估哮喘治疗10的有效性。同时，哮喘病理生理学的研究需要测试样本，包括气管、肺和支气管厕所的样本，以验证关键因素11水平的变化。哮喘的老鼠模型通常用于评估哮喘病理生理学，因为它们产生持久的气道反应，并显示即时和后期气道反应12。然而，由于反复的实验刺激，大鼠往往变得易怒。因此，需要一种合适的方法来修复老鼠。常用的老鼠固定方法包括麻醉和结合13。虽然麻醉方法提供了更好的暴露穴位，它可能会影响神经传导，并最终影响实验结果14。结合方法不会诱导与麻醉相同的生理效果，但受约束的老鼠会经历骨骼肌肉紧张。此外，针头不能轻易到达指定位置15。在这里，我们引入了一个高平台，可用于修复大鼠，而无需限制它们，并充分暴露背面的穴位。在针灸管理期间，大鼠可以放在高平台上。高平台只能容纳老鼠，因为它被放置在离地面一定距离的地方，老鼠不会移动，因为害怕高度16。
本文详细描述了高平台的制造、哮喘鼠模型的建立、高平台上需要大鼠的操作以及使用全身全息摄影（WBP）系统测量呼吸功能。

<table><tbody><tr><td>0.3 mm x 13.0 mm acupuncture needle</td><td>China Suzhou Medical Device Factory</td><td></td><td></td></tr><tr><td>Al(OH)&lt;sub&gt;3&lt;/sub&gt;</td><td>American Thermo</td><td>77161</td><td></td></tr><tr><td>Compression nebulizer</td><td>Jiangsu Lude Medical Electronics Co., Ltd.</td><td>NB-212C</td><td></td></tr><tr><td>Fine Pointe V2.0</td><td>American Buxco</td><td></td><td></td></tr><tr><td>Laboratory gas drying unit</td><td>American Drierite</td><td>26800</td><td></td></tr><tr><td>Methacholine</td><td>American Sigma</td><td>MKCF6054</td><td></td></tr><tr><td>Ovalbumin</td><td>American Sigma</td><td>A5503-25G</td><td></td></tr><tr><td>Phosphate Buffer solution (1x)</td><td>Gelifesciences</td><td>SH30256.01</td><td></td></tr><tr><td>Seal box</td><td>IRIS Corporation of Japan</td><td></td><td></td></tr><tr><td>Whole-body plethysmography respiratory function measurement system</td><td>American Buxco</td><td></td><td></td></tr></tbody></table>

acupuncture in a rat model of asthma

高平台可以不受限制地修复大鼠，并在针灸操作期间完全暴露背部的穴位。本文描述了制造高平台的方法，建立了哮喘的老鼠模型，并使用非侵入性和实时全身全息摄影（WBP）系统测量呼吸功能的变化。

在GV14、双边BL12和双边BL13点进行针灸时，大鼠保持一个容易的位置。老鼠只能把头扭到外面，不能扭到里面，因为一个方向被木板挡住了（图5）。
随着甲基苯甲酰浓度的增加，Penh 呈现出上升趋势，增长率逐渐增加。浓度为3.125毫克/千升、6.25毫克/千升、12.5毫克/mL、25毫克/mL和50毫克/千升， Penh 增长 18.40% ± 10.70%， 27.55% ± 26.30%， 88.10% ± 45.63 %，427.60%±17...

Watch this Scientific Journal Video about Acupuncture in a Rat Model of Asthma at JoVE.com

Acupuncture in a Rat Model of Asthma

哮喘鼠模型中的针灸

A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes ...

Research

JoVE Journal

Medicine

5.9K 次观看.  Shanghai University of Traditional Chinese Medicine. 高平台可以不受限制地修复大鼠，并在针灸操作期间完全暴露背部的穴位。本文描述了制造高平台的方法，建立了哮喘的老鼠模型，并使用非侵入性和实时全身全息摄影（WBP）系统测量呼吸功能的变化。

视频: Acupuncture in a Rat Model of Asthma

Murine Model of Allergen Induced Asthma

Asthma is a major cause of morbidity and mortality, affecting some 300 million people throughout the world.1 More than 8% of the US population has asthma, with the prevalence increasing.2 As with other diseases, animal models of allergic airway disease greatly facilitate understanding of the underlying pathophysiology, help identify potential therapeutic targets, and allow preclinical testing of possible new therapies. Models of allergic airway disease have been developed in several animal species, but murine models are particularly attractive due to the low cost, ready availability, and well-characterized immune systems of these animals.3 Availability of a variety of transgenic strains further increases the attractiveness of these models.4 Here we describe two murine models of allergic airway disease, both employing ovalbumin as the antigen. Following initial sensitization by intraperitoneal injection, one model delivers the antigen challenge by nebulization, the other by intratracheal delivery. These two models offer complementary advantages, with each mimicking the major features of human asthma.5
The major features of acute asthma include an exaggerated airway response to stimuli such as methacholine (airway hyperresponsiveness; AHR) and eosinophil-rich airway inflammation. These are also prominent effects of allergen challenge in our murine models,5,6 and we describe techniques for measuring them and thus evaluating the effects of experimental manipulation. Specifically, we describe both invasive7 and non-invasive8 techniques for measuring airway hyperresponsiveness as well as methods for assessing infiltration of inflammatory cells into the airways and the lung. Airway inflammatory cells are collected by bronchoalveolar lavage while lung histopathology is used to assess markers of inflammation throughout the organ. These techniques provide powerful tools for studying asthma in ways that would not be possible in humans.

Experimental mouse models of allergic asthma offer new possibilities for studying disease pathogenesis and developing new therapeutics. These models are well suited to measuring factors governing the allergic immune response, airway inflammation, and pulmonary pathophysiology.

过敏原诱发的哮喘小鼠模型

Asthma is a major cause of morbidity and mortality, affecting some 300 million people throughout the world.1 More than 8% of the US population has asthma, ...

科研

免疫与感染

针灸慢性缺氧小鼠的认知改善

Acupuncture Treatment in a Mouse Model of Chronic Hypoxia-Induced Cognitive Dysfunction

The treatment of central nervous disorders has consistently posed significant challenges to the medical field. Acupuncture, a non-pharmacological practice rooted in traditional Chinese medicine, entails the insertion of fine needles into precise points on the body and is commonly employed for the management of diverse conditions. Recently, acupuncture has emerged as a promising therapeutic intervention for a range of neurological diseases, including anxiety and respiratory disorders. However, the potential of acupuncture in treating cognitive dysfunction induced by chronic hypoxia has not yet been explored. This paper presents a comprehensive protocol for establishing a mouse model of chronic hypoxia-induced cognitive impairment, administering mild anesthesia, performing acupuncture treatment, and assessing behavioral changes and memory abilities using open field tests and water mazes. The step-by-step protocol provides detailed instructions on accurately locating and positioning acupoints and needles for cognitive improvement. By employing this protocol, researchers can conduct systematic studies to thoroughly evaluate the therapeutic potential of acupuncture for cognitive dysfunction.

作者聚焦:改进增强小鼠针刺研究的麻醉方案

Here, we describe a protocol for implementing mild anesthesia and acupuncture treatment on a chronic hypoxia mouse model and conducting behavioral tests to assess the cognitive alterations post-treatment.

针灸治疗慢性缺氧诱导认知功能障碍小鼠模型

The treatment of central nervous disorders has consistently posed significant challenges to the medical field. Acupuncture, a non-pharmacological practice ...

行为学

<meta charset="utf8"></head><body>评估推拿疗法对慢性缩窄大鼠的疼痛缓解效果

Characteristics of Pain Changes in Rats with Nerve Injury Within 24 hours After One-Time Tuina Intervention

Tuina, as an external treatment method of traditional Chinese medicine, has been proven to have an analgesic effect on peripheral neuropathic pain (pNP) in clinical and basic research. However, the optimal time point for the analgesic effect of tuina may vary according to different injury sensations, affecting the exploration of the initiation mechanism of tuina analgesia. 
The research used minor chronic constriction injury (minor CCI) model rats to simulate pNP and used the intelligent tuina manipulation simulator to simulate the three methods (point-pressing, plucking, and kneading) and three acupoints (Yinmen BL37, Chengshan BL57, and Yanglingquan GB34) for performing tuina therapy. The study evaluated the changes in pain within 24 h and the optimal time point for the efficacy of tuina analgesia in rats with minor CCI models by testing cold sensitivity threshold (CST), mechanical withdrawal threshold (MWT), and thermal withdrawal latency (TWL). Furthermore, the study evaluated IL-10 and TNF-&#945; expression changes through Elisa detection. The results show that tuina has both immediate and sustained analgesic effects. For the three different injury sensitivity thresholds of CST, MWT, TWL, and two cytokines of IL-10 and TNF-&#945;, the analgesic efficacy of tuina within 24 h after intervention is significantly different at different time points.

<meta charset="utf8"></head><body>作者聚焦：探索神经损伤大鼠“三法三穴”推拿镇痛的启动机制

We present a protocol using the tuina manipulation simulator to perform the "Three-Manipulation and Three-Acupoint" tuina therapy for minor chronic constriction injury rats and evaluate the effective analgesic time points of tuina within 24 h by testing pain changes through behavioral analysis and changes in inflammatory factor expression using enzyme-linked immunosorbent assay.

神经损伤大鼠1次推拿干预后24 h内疼痛变化特征

Tuina, as an external treatment method of traditional Chinese medicine, has been proven to have an analgesic effect on peripheral neuropathic pain (pNP) ...

神经科学

A Traditional Chinese Medicine Characteristic Therapy for Bronchial Asthma: Moxibustion

Bronchial asthma is a chronic inflammatory disease of the airway which can lead to symptoms such as recurrent wheezing, shortness of breath, chest tightness, or cough, as a result of increased airway reactivity. With high diurnal variation, these symptoms often occur or worsen at night or in the morning. By burning and roasting the Chinese medica above human acupoints, moxibustion is a type of treatment that stimulates the activity of the human meridians through drugs and heat stimulation to prevent and treat diseases. According to the principle of syndrome differentiation and treatment of traditional Chinese medicine, acupoints are selected on the corresponding parts, which has a definite effect. It is regarded as a characteristic traditional Chinese medicine therapy for bronchial asthma. This protocol elaborates the methods and steps of patient management, material preparation, selection of acupoints, operation, and postoperative nursing to ensure safe and effective moxibustion treatment to significantly improve the clinical symptoms and quality of life of patients with bronchial asthma.

This protocol presents a curative procedure of moxibustion in treating patients with bronchial asthma.

支气管哮喘的中医特色疗法:艾灸

Bronchial asthma is a chronic inflammatory disease of the airway which can lead to symptoms such as recurrent wheezing, shortness of breath, chest ...

医学

改进大鼠模型中的 FSN 治疗:最大限度地减少不适并最大限度地提高效率

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the mechanisms of action are still not fully understood. During FSN treatment, the FSN needle is inserted and retained in the subcutaneous tissues for a long duration with a swaying movement. However, challenges arise from maintaining a posture while manipulating FSN in animal models (e.g., rats) for researchers. Uncomfortable treatment can lead to fear and resistance to FSN needles, increasing the risk of injury and may even affect research data. Anesthesia may also affect the study results too. Hence, there is a need for strategies in FSN therapy on animals that minimize injury during the intervention. This study employs a chronic constriction injury model in Sprague-Dawley rats to induce neuropathic pain. This model replicates the pain induced by nerve injury observed in humans through surgical constriction of a peripheral nerve, mimicking the compression or entrapment seen in conditions such as nerve compression syndromes and peripheral neuropathies. We introduce an appropriate manipulation for easily inserting an FSN needle into the subcutaneous layer of the animal&#39;s body, including needle insertion and direction, needle retention, and swaying movement. Minimizing the rat&#39;s discomfort prevents the rat from being tense, which causes the muscle to contract and hinder the entry of the needle and improves the study efficiency.

作者聚焦:揭示 FSN 治疗的治疗效果 – 桥接神经性疼痛的研究和临床应用

We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats.

傅氏皮下针刺治疗坐骨神经疼痛的疗效:慢性缩窄损伤大鼠模型中的行为和电生理变化

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the ...

哮喘鼠模型中的针灸

摘要

探索更多视频

此视频中的章节

过敏原诱发的哮喘小鼠模型

针灸治疗慢性缺氧诱导认知功能障碍小鼠模型

针灸治疗慢性缺氧诱导认知功能障碍小鼠模型

针灸治疗慢性缺氧诱导认知功能障碍小鼠模型

神经损伤大鼠1次推拿干预后24 h内疼痛变化特征

神经损伤大鼠1次推拿干预后24 h内疼痛变化特征

神经损伤大鼠1次推拿干预后24 h内疼痛变化特征

支气管哮喘的中医特色疗法:艾灸

傅氏皮下针刺治疗坐骨神经疼痛的疗效:慢性缩窄损伤大鼠模型中的行为和电生理变化

傅氏皮下针刺治疗坐骨神经疼痛的疗效:慢性缩窄损伤大鼠模型中的行为和电生理变化