January 13th, 2022
•In this protocol, AAV2 vector is produced by co-culturing Spodoptera frugiperda (Sf9) insect cells with baculovirus (BV)-AAV2-green fluorescent protein (GFP) or therapeutic gene and BV-AAV2-rep-cap infected Sf9 cells in suspension culture. AAV particles are released from the cells using detergent, clarified, purified by affinity column chromatography, and concentrated by tangential flow filtration.
Related Videos
The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
The MultiBac Protein Complex Production Platform at the EMBL
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
An Ex vivo Culture System to Study Thyroid Development
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus
A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System