Surgical Preparation and Procedures of Liver Perfusion
2:08
Tissue Decellularization and Slide Preparation for Imaging
3:35
Image Acquisition
4:58
Results: Two-Photon Microscopy of the Liver Extracellular Matrix in a Mouse
6:19
Conclusion
副本
NASH is one of the most important chronic liver disease in the world. It can progress to fibrosis, cirrhosis, and eventually the liver cancer. Study the dynamic change of the ECM in the NASH can help to understand the fibrosis better.
Tradit
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The present protocol optimizes the liver in situ perfusion/decellularization and two-photon microscopy methods to establish a reliable platform to visualize the dynamics of extracellular matrix (ECM) remodeling during non-alcoholic steatohepatitis (NASH).