Embryo Incubation and Preparation of Culture Dishes
1:49
Isolation of Chick Embryos
3:01
Dissecting Neural Folds
4:22
Plating Neural Folds
5:20
Fixing and Staining of Cultured Migratory Neural Crest Cells for Morphological Analysis
7:08
Results: Assessment of Cultured Migratory Neural Crest Cells
8:39
Conclusion
副本
Neural crest cells emigrate from cranial neural folds in embryos or when placed in culture. This provides a convenient method to isolate primary migratory neural crest cells without cell dissociation. While neural crest migration typically occurs
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This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.
