Fabricating the Master Wafer and Preparing the Microfluidic Device
3:46
Making the PDMS-Coated Glass Slide and Bonding the Microfluidic Device
5:24
Surface Functionalization of the Microfluidic Device
6:48
Liposome Production
7:33
Results: Production of Monodispersed Liposomes and Subsequent pH-Triggered Liquid-Liquid Phase Separation of pLL/ATP Inside Liposomes
9:02
Conclusion
副本
OLA is a versatile microfluidic platform useful to the synthetic biology community. in particular, to bioengineer artificial cells. OLA-based liposomes can help us understand the self-organization principles in biology and construct cell-mimicking
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The present protocol describes octanol-assisted liposome assembly (OLA), a microfluidic technique to generate biocompatible liposomes. OLA produces monodispersed, micron-sized liposomes with efficient encapsulation, allowing immediate on-chip experimentation. This protocol is anticipated to be particularly suitable for synthetic biology and synthetic cell research.
