<meta charset="utf8"></head><body>用于再生研究的标准化大鼠颅骨缝合骨缺损模型

<ol>
	<li>Li, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cranial+suture+mesenchymal+stem+cells:+insights+and+advances.">Cranial suture mesenchymal stem cells: insights and advances.</a> Biomolecules. 11 (8), 1129 (2021).</li><li>Opperman, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cranial+sutures+as+intramembranous+bone+growth+sites.">Cranial sutures as intramembranous bone growth sites.</a> Dev Dyn. 219 (4), 472-485 (2000).</li><li>Lenton, K. A., Nacamuli, R. P., Wan, D. C., Helms, J. A., Longaker, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cranial+suture+biology.">Cranial suture biology.</a> Curr Top Dev Biol. 66 (4), 287-328 (2005).</li><li>Slater, B. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cranial+sutures:+A+brief+review.">Cranial sutures: A brief review.</a> Plast Reconstr Surg. 121 (4), 170-178 (2008).</li><li>Zhao, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+suture+provides+a+niche+for+mesenchymal+stem+cells+of+craniofacial+bones.">The suture provides a niche for mesenchymal stem cells of craniofacial bones.</a> Nat Cell Biol. 17 (4), 386-396 (2015).</li><li>Maruyama, T., Jeong, J., Sheu, T. -. J., Hsu, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stem+cells+of+the+suture+mesenchyme+in+craniofacial+bone+development,+repair+and+regeneration.">Stem cells of the suture mesenchyme in craniofacial bone development, repair and regeneration.</a> Nat Commun. 7 (1), 10526 (2016).</li><li>Wilk, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postnatal+calvarial+skeletal+stem+cells+expressing+PRX1+reside+exclusively+in+the+calvarial+sutures+and+are+required+for+bone+regeneration.">Postnatal calvarial skeletal stem cells expressing PRX1 reside exclusively in the calvarial sutures and are required for bone regeneration.</a> Stem Cell Reports. 8 (4), 933-946 (2017).</li><li>Doro, D. H., Grigoriadis, A. E., Liu, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calvarial+suture-derived+stem+cells+and+their+contribution+to+cranial+bone+repair.">Calvarial suture-derived stem cells and their contribution to cranial bone repair.</a> Front Physiol. 8, 956 (2017).</li><li>Kajdic, N., Spazzapan, P., Velnar, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Craniosynostosis-recognition,+clinical+characteristics,+and+treatment.">Craniosynostosis-recognition, clinical characteristics, and treatment.</a> Bosn J Basic Med Sci. 18 (2), 110-116 (2018).</li><li>Park, S., Zhao, H., Urata, M., Chai, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sutures+possess+strong+regenerative+capacity+for+calvarial+bone+injury.">Sutures possess strong regenerative capacity for calvarial bone injury.</a> Stem Cells Dev. 25 (23), 1801-1807 (2016).</li><li>Quarto, N., Behr, B., Longaker, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Opposite+spectrum+of+activity+of+canonical+Wnt+signaling+in+the+osteogenic+context+of+undifferentiated+and+differentiated+mesenchymal+cells:+Implications+for+tissue+engineering.">Opposite spectrum of activity of canonical Wnt signaling in the osteogenic context of undifferentiated and differentiated mesenchymal cells: Implications for tissue engineering.</a> Tissue Eng Part A. 16 (10), 3185-3197 (2010).</li><li>Kaku, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+Stem+Cell-Induced+Cranial+Suture-Like+Gap+in+Rats.">Mesenchymal Stem Cell-Induced Cranial Suture-Like Gap in Rats.</a> Plast Reconstr Surg. 127 (1), 69-77 (2011).</li><li>Yu, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cranial+suture+regeneration+mitigates+skull+and+neurocognitive+defects+in+craniosynostosis.">Cranial suture regeneration mitigates skull and neurocognitive defects in craniosynostosis.</a> Cell. 184 (1), 243-256 (2021).</li><li>Koons, G. L., Diba, M., Mikos, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Materials+design+for+bone-tissue+engineering.">Materials design for bone-tissue engineering.</a> Nat Rev Mater. 5 (8), 584-603 (2020).</li><li>Mardas, N., Kostopoulos, L., Karring, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bone+and+suture+regeneration+in+calvarial+defects+by+e-PTFE-membranes+and+demineralized+bone+matrix+and+the+impact+on+calvarial+growth:+an+experimental+study+in+the+rat.">Bone and suture regeneration in calvarial defects by e-PTFE-membranes and demineralized bone matrix and the impact on calvarial growth: an experimental study in the rat.</a> J Craniofac Surg. 13 (3), 453-462 (2002).</li><li>Kostopoulos, L., Karring, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regeneration+of+the+sagittal+suture+by+GTR+and+its+impact+on+growth+of+the+cranial+vault.">Regeneration of the sagittal suture by GTR and its impact on growth of the cranial vault.</a> J Craniofac Surg. 11 (6), 553-561 (2000).</li><li>Mosaddad, S. A., Hussain, A., Tebyaniyan, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exploring+the+use+of+animal+models+in+craniofacial+regenerative+medicine:+A+narrative+review.">Exploring the use of animal models in craniofacial regenerative medicine: A narrative review.</a> Tissue Eng Part B Rev. , (2023).</li><li>Tan, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PgC3Mg+metal-organic+cages+functionalized+hydrogels+with+enhanced+bioactive+and+ROS+scavenging+capabilities+for+accelerated+bone+regeneration.">PgC3Mg metal-organic cages functionalized hydrogels with enhanced bioactive and ROS scavenging capabilities for accelerated bone regeneration.</a> J Mater Chem B. 10 (28), 5375-5387 (2022).</li><li>Yazdanian, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+and+properties+of+&#946;TCP/Zeolite/Gelatin+scaffold+as+developed+scaffold+in+bone+regeneration:+in+vitro+and+in+vivo+studies.">Fabrication and properties of &#946;TCP/Zeolite/Gelatin scaffold as developed scaffold in bone regeneration: in vitro and in vivo studies.</a> Biocybern Biomed Eng. 40 (4), 1626-1637 (2020).</li><li>Soufdoost, R. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+and+in+vivo+evaluation+of+novel+Tadalafil/&#946;-TCP/Collagen+scaffold+for+bone+regeneration:+A+rabbit+critical-size+calvarial+defect+study.">In vitro and in vivo evaluation of novel Tadalafil/&#946;-TCP/Collagen scaffold for bone regeneration: A rabbit critical-size calvarial defect study.</a> Biocybern Biomed Eng. 39 (3), 789-796 (2019).</li><li>Russell, W. P., Russell, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anatomy,+head+and+neck,+coronal+suture.">Anatomy, head and neck, coronal suture.</a> StatPearls. , (2022).</li><li>Menon, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Skeletal+stem+and+progenitor+cells+maintain+cranial+suture+patency+and+prevent+craniosynostosis.">Skeletal stem and progenitor cells maintain cranial suture patency and prevent craniosynostosis.</a> Nat Commun. 12 (1), 4640 (2021).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>4% paraformaldehyde</td>
			<td>Biosharp</td>
			<td>BL539A</td>
			<td></td>
		</tr>
		<tr>
			<td>2% Iodophor solution</td>
			<td>Chengdu Jinshan Chemical Reagent Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>75% Ethanol</td>
			<td>Chengdu Jinshan Chemical Reagent Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton balls</td>
			<td>Haishi Hainuo Group Co., Ltd.&#160;</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton swabs</td>
			<td>Lakong Medical Devices Co.,&#160;</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Curved forceps</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Dataviewer and Ctan software for residual defect volume assessments</td>
			<td>Bruker</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Dental low-speed round burs</td>
			<td>Dreybird Medical Equipment Co., Ltd.</td>
			<td>RA3-012 
			RA1-008</td>
			<td></td>
		</tr>
		<tr>
			<td>Disposable sterile scalpel</td>
			<td>Hangzhou Huawei Medical Supplies Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Disposable syringes (22 G)</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>SB1-089(IX)</td>
			<td></td>
		</tr>
		<tr>
			<td>Electric shaver</td>
			<td>JASE</td>
			<td>BM320210</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethylene Diamine Tetraacetic Acid (EDTA)</td>
			<td>BioFroxx</td>
			<td>1340GR500</td>
			<td></td>
		</tr>
		<tr>
			<td>Hematoxylin and Eosin Stain Kit</td>
			<td>Biosharp</td>
			<td>BL700B</td>
			<td></td>
		</tr>
		<tr>
			<td>Irrigation needle (23 G)</td>
			<td>Sichuan New Century Medical Polymer Products Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Low-speed handpiece</td>
			<td>Guangzhou Dental Guard Technology Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Masson&#8217;s Trichrome Stain Kit</td>
			<td>Solarbio</td>
			<td>G1340</td>
			<td></td>
		</tr>
		<tr>
			<td>Medical non-woven fabrics</td>
			<td>Henan Yadu Industrial Co., Ltd.&#160;</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro-computed tomography (&#181;CT)&#160;</td>
			<td>Scanco Medical AG</td>
			<td>&#181;CT45</td>
			<td></td>
		</tr>
		<tr>
			<td>Mimics 20.0 for cross-sectional images</td>
			<td>Materialise</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Needle holders</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Periosteal elevator</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Saline solution</td>
			<td>Sichuan Kelun Pharmaceutical Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Scanco medical visualizer software for 3D image reconstruction</td>
			<td>Scanco Medical AG</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>SPSS Statistics 20.0 for statistical analysis</td>
			<td>IBM</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Sprague-Dawley rats&#160;</td>
			<td>Byrness Weil Biotech Ltd</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Straight Scissors</td>
			<td>Chengdu Shifeng Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical Motor</td>
			<td>MARATHON</td>
			<td>N3-140232</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical sutures (3-0 monofilament)</td>
			<td>Hangzhou Huawei Medical Supplies Co., Ltd.</td>
			<td>None</td>
			<td></td>
		</tr>
	</tbody>
</table>

the establishment of calvarial suture-bony composite defects in rats: a standardized model for suture-regenerative therapy investigation

Cranial sutures are dense fibrous connections between cranial bones, acting as joints to facilitate slight skull movement and providing a protective cushion for the brain under pressure1. In recent years, increased research has highlighted the pivotal role of cranial sutures in skull development, craniofacial homeostasis, and inherent osteo-reparative potential2,3,4,5,6,7,8. During periods of growth and development, cranial sutures act as the main growth centers in the skull4. New bone formation occurs at the osteogenic fronts on both sides of the sutures, while the cells within the sutures maintain an undifferentiated mesenchymal state, ensuring balanced skull expansion alongside brain growth1. The loss of cranial sutures at this time results in a discrepancy between the growth of the skull and brain, leading to severe issues like brain injuries, hydrocephalus, increased intracranial pressure, and cognitive dysfunction3,9.
Besides, cranial sutures play a crucial role in determining the prognosis of calvarial defects5,7,10. The regenerative potential across the calvarial surface is unevenly distributed, with cranial sutures showing remarkably superior capabilities compared to non-suture regions10,11. One study indicates that the speed of calvarial defect healing inversely correlates with the distance between the cranial suture and the injury site10. Specifically, the removal of coronal and sagittal sutures leads to the non-healing of parietal bone defects7, emphasizing the necessity of suture regeneration in calvarial defects. However, the focus of the current studies is predominantly on the restoration of cranial osseous structure while neglecting the regeneration of suture mesenchyme.
Regarding advancements in suture regeneration, promising outcomes have been observed with the transplantation of suture-containing bone flaps, mesenchymal stem cells (MSCs), and artificial biomaterials. When bone flaps with sutures were transplanted into calvarial defects, they successfully integrated and healed, in contrast to those without sutures displaying non-union and an inability to form periosteum, dura mater, or osteocytes5. Likewise, the implantation of MSCs derived from bone marrow into sagittal suture-bony composite defects facilitated the formation of suture-like gaps12. Of note, a recent study highlighted the realization of suture regeneration with Gli1+ MSCs, allowing for intracranial pressure control, skull deformity correction, and enhanced neurocognitive function13. As regenerative medicine and biomedical engineering develop, researchers increasingly focus on tissue engineering biomaterials due to their adaptable and customizable characteristics14. Notably, polytetrafluoroethylene membranes have been proven to be effective in reconstructing cranial bone and suture mesenchyme simultaneously15,16.
However, craniofacial research lacks established models for exploring suture mesenchymal regenerative therapies, unlike relatively mature models in repairing other tissues such as bone, skin, cartilage, and muscles17. The absence of a standardized model constrains the study of suture-regenerative therapies and makes it challenging to perform comparative analysis across different studies. Therefore, our study established a practicable and reproducible rat calvarial suture-bony composite defect. Through this method, we aim to develop appropriate clinical interventions for cranial suture reconstruction, offering novel perspectives on the functional repairment of calvarial defects and decreasing unfavorable outcomes resulting from suture loss.

<meta charset="utf8"></head><body>作者聚焦：颅骨缝合骨复合缺陷标准化大鼠模型的开发和评估

大鼠颅骨缝合骨复合缺损的建立：缝合再生治疗研究的标准化模型

Our research focuses on the detailed surgical procedures of calvarial suture-bony composite defects in rats alongside the investigations into the short-term and long-term prognoses of the model. The primary objective is to establish a standardized model that can be utilized for advancing suture regenerative therapies. For the current animal model, the primary challenge lies in completely removing the coronal suture while preserving the sagittal and frontal sutures with minimal osseous tissue removal.Our protocol allows for more straightforward comparation through self-control by creating defects in both the left and the right halves of the coronal suture and applying different interventions to each side.

Read this Scientific Article Protocol about The Establishment of Calvarial Suture-Bony Composite Defects in Rats: A Standardized Model for Suture-Regenerative Therapy Investigation at JoVE.com

Large-scale calvarial defects often coincide with cranial suture disruption, leading to impairments in calvarial defect restoration and skull development ...

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Research

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Medicine

The Establishment of Calvarial Suture-Bony Composite Defects in Rats: A Standardized Model for Suture-Regenerative Therapy Investigation

262 次观看.  Sichuan University. 我们的研究重点是大鼠颅骨缝合骨复合缺损的详细外科手术，以及对模型的短期和长期预后的研究。主要目标是建立一个可用于推进缝合再生疗法的标准化模型。对于当前的动物模型，主要挑战在于完全去除冠状缝合，同时保留矢状缝和额缝，同时以最少的骨组织去除。我们的方案允许在冠状缝合线的左半部分和右半部分产生缺陷，并对每一侧...