单色多重定量 PCR 端粒长度测量

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11:44 min

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March 22nd, 2024

DOI :

10.3791/66545-v

March 22nd, 2024

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Noelle A. Martin*¹, Lauren W. Y. McLester-Davis*²^,³^,⁴, Trevor R. Roy¹, Madeline G. Magruder⁵, Waylon J. Hastings¹, Stacy S. Drury⁶

¹School of Medicine, Tulane University, ²Native American Center for Health Professions, University of Wisconsin-Madison, ³Department of Medicine, University of Wisconsin-Madison, ⁴Department of Biochemistry, University of Wisconsin-Madison, ⁵School of Science and Engineering, Tulane University, ⁶Department of Psychiatry and Behavioral Sciences, Boston Children’s Hospital

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PCR MMqPCR DNA

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0:00

Introduction

1:49

Sample and Master Mix Preparation for Telomere Length Measurement Using Monochrome Multiplex Quantitative PCR (MMqPCR)

3:42

Preparation of 96‐Well Plate for Monochrome Multiplex Quantitative PCR (MMqPCR) for Telomere Length Measurement